Human MIAPaCa-2 and PANC-1 pancreatic cancer cells were purchased from the American Type Culture Collection (ATCC, VA, USA). MIA PaCa-2 and PANC-1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Gyeongsan, Korea) with 1% antibiotic-antimycotic and 10% fetal bovine serum (FBS). Cultures were maintained in an incubator with 95% air and 5% CO2 at 37°C.
Mia paca 2
Manufactured by Welgene
MIA PaCa-2 is a cell line derived from a human pancreatic carcinoma. It is commonly used in research applications to study pancreatic cancer.
Automatically generated - may contain errors
Lab products found in correlation
Mia paca 2, by American Type Culture Collection (3 mentions)
Panc 1, by Welgene (3 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (2 mentions)
Bxpc 3, by American Type Culture Collection (2 mentions)
Bxpc 3, by Welgene (2 mentions)
Rpmi 1640 medium, by Welgene (2 mentions)
Panc 1, by American Type Culture Collection (2 mentions)
Aspc 1, by American Type Culture Collection (2 mentions)
Aspc 1, by Welgene (2 mentions)
Dmem medium, by Welgene (1 mentions)
Mia paca 2, by Korean Cell Line Bank (1 mentions)
Gemcitabine, by Merck Group (1 mentions)
Capan 1, by American Type Culture Collection (1 mentions)
Capan 1, by Welgene (1 mentions)
5 aza dc, by Merck Group (1 mentions)
Cfpac 1, by Welgene (1 mentions)
Cfpac 1, by American Type Culture Collection (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Suit 2 cells, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Iscove modified dulbecco media (imdm), by Welgene (1 mentions)
5 protocols using mia paca 2
1
Cultivation of Pancreatic Cancer Cell Lines
2
Establishment and Culturing of Isogenic Cell Lines
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KrasG12D and KrasWT mouse embryonic fibroblasts (MEFs) (a gift from Dr. Craig B. Thompson, Memorial Sloan Kettering Cancer Center, New York, NY, USA) were obtained from Cre recombinase-induced SV40 large T-immortalized Lox-Stop-Lox-K-RasG12D MEF [14 (link)]. MEFs and the pancreatic cancer cell line MIA PaCa-2 were cultured in DMEM medium (WELGENE, Gyeongsan-si, Korea) containing 10% fetal bovine serum and 1% penicillin/streptomycin. Another pancreatic cancer cell line, BXPC-3, and lung cancer cell lines A549 and H522 were cultured in RPMI 1640 medium (WELGENE) containing 10% fetal bovine serum and 1% penicillin/streptomycin. For leucine deprivation, cells were maintained in leucine-free DMEM or RPMI 1640 containing 10% dialyzed fetal bovine serum (FBS). MIA PaCa-2, A549, and H522 were obtained from Korean Cell Line Bank (Seoul, Korea) and BXPC-3 was obtained from the American Type Culture Collection (Manassas, VA, USA).
3
Pancreatic Cancer Cell Lines Characterization
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Pancreatic cancer cell lines, AsPC-1, MiaPaCa-2, Panc-1, BxPC-3 and Capan-1 were obtained from the American Type Culture Collection (ATCC, USA). AsPC-1, BxPC-3 and Capan-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and 1% antibiotic (Welgene, South Korea), while MiaPaCa-2 and Panc-1 cells were cultured in DMEM with 10% FBS and 1%. Cancer cell lines were authenticated by short tandem repeat (STR) DNA profiling analysis. Recombinant human Wnt3a and Wnt7a proteins were purchased from R&D Systems (USA). PG545 was provided by Progen Pharmaceuticals (Australia) and gemcitabine was from Sigma Chemical (USA). The recombinant His-tagged Wnt3a and Wnt7a proteins were from Proteintech Group (USA).
4
Epigenetic Modulation of Pancreatic Cancer
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Five human pancreatic cancer cell lines (AsPC-1, CFPAC-1, MIA PaCa-2, PANC-1, and SUIT-2) were used in this study. AsPC-1, CFPAC-1, MIA PaCa-2, and PANC-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). SUIT-2 cells were purchased from the Japanese Collection of Research Bioresources (JCBR, Japan) cell bank (The National Institute of Biomedical Innovation, Health and Nutrition (NIBIOHN), Japan). Cell line AsPC-1 was cultured in RPMI-1640 (Welgene, Daegu, Korea); CFPAC-1 cells were maintained in Iscove's Modified Dulbecco's Medium (IMDM, Welgene); and MIA PaCa-2, PANC-1, and SUIT-2 cells were grown in Dulbecco's Modified Eagle Medium (DMEM, Welgene). All cell culture medium was supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and 1% antibiotic-antimycotic (Gibco, Grand Island, NY, USA). All cell lines were incubated at 37°C in atmospheric conditions of 20% O2 and 5% CO2. To investigate the effect of 5-aza-dC treatment, cells were treated with 5 μM 5-aza-dC (Sigma, St. Louis, MO, USA) for 72 h.
5
Combination Therapy for Pancreatic Cancer
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Cells and reagents. The MiaPaca-2 human pancreatic carcinoma cell line was obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). MiaPaca-2 cells were grown in Dulbecco's modified Eagle's medium (DMEM, Welgene, Gyeongsan, Korea) with 1% streptomycin and penicillin (Corning, Corning, NY, USA), and 10% fetal bovine serum (Tissue Culture Biologicals, Tulare, CA, USA) at 37˚C and 5% CO 2 in a humidified environment. Compounds [50 μM fisetin (TOCRIS, Bristol, UK) with/without 100 nM gemcitabine (Yuhan, Seoul, Korea)] were added, and the cells were incubated as described below. Cells were treated with dimethyl sulfoxide (DMSO) (0.25%) as a control.
Polyclonal antibodies against c-MYC, phospho-ERK1/2 and ERK1/2 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Polyclonal α-tubulin (LF-PA0146) was purchased from Abfrontier (Seoul, Korea). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG Ab (sc-2004) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
Polyclonal antibodies against c-MYC, phospho-ERK1/2 and ERK1/2 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Polyclonal α-tubulin (LF-PA0146) was purchased from Abfrontier (Seoul, Korea). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG Ab (sc-2004) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
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