Dmem high glucose

Manufactured by Dutscher

Sourced in France

DMEM-High Glucose is a cell culture medium that provides a balanced salt solution and essential nutrients for the growth and maintenance of various cell types in vitro. It contains a high concentration of glucose, which serves as the primary energy source for cells.

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Lab products found in correlation

Penicillin streptomycin, by Corning (2 mentions) Fetal bovine serum (fbs), by PAN Biotech (2 mentions) Series 2 water jacker, by Thermo Fisher Scientific (2 mentions) Sw480, by American Type Culture Collection (1 mentions) Sw620, by American Type Culture Collection (1 mentions) Dld 1, by American Type Culture Collection (1 mentions) Lsm 800 microscope, by Zeiss (1 mentions) Jetpei transfection reagent, by Polyplus Transfection (1 mentions) Hcs lipidtox deep red neutral lipid stain, by Thermo Fisher Scientific (1 mentions) Doxycycline, by Merck Group (1 mentions) L glutamine, by Cytiva (1 mentions)

Spelling variants of this product

High-glucose DMEM medium (5 citations)

4 protocols using dmem high glucose

Colorectal Cancer Cell Culture Protocols

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Colorectal cancer cells (DLD-1, HT-29, SW480, and SW620) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA). Table 5 shows the main differential characteristics of the colon cancer lines used in this study.
Cells lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-High Glucose, Dominique Dutscher, Bernolsheim, France), 10% fetal bovine serum (FBS, PAN Biotech, Aidenbach, Germany), 2 mM L-glutamine (FBS, PAN Biotech, Germany), and 1% penicillin/streptomycin (Corning, New York, NY, USA) at 37 °C in a humidified incubator (Series II water Jacker, Thermo Scientific, Waltham, MA, USA) with 5% CO₂.

Anglana C., Rojas M., Girelli C.R., Barozzi F., Quiroz-Troncoso J., Alegría-Aravena N., Montefusco A., Durante M., Fanizzi F.P., Ramírez-Castillejo C, & Di Sansebastiano G.P. (2023). Methanolic Extracts of D. viscosa Specifically Affect the Cytoskeleton and Exert an Antiproliferative Effect on Human Colorectal Cancer Cell Lines, According to Their Proliferation Rate. International Journal of Molecular Sciences, 24(19), 14920.

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Lipid Droplet Biogenesis in HeLa Cells

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HeLa cells were maintained in DMEM High Glucose (Dutscher) with 10% FBS and 1% penicillin–streptomycin at 37°C with 5% CO₂ and seeded on MatTek 35-mm coverslip bottom dishes for at least 16 h before transfection. Cells were transfected with the different plasmids by using jetPEI transfection reagent (#101-10N; PolyPlus) for 24 h. For the TG-rich LD experiments, cells were then incubated for 24 h with DMEM supplemented with OA conjugated to BSA (1% vol/vol) for a final concentration of 400 µM. The SE-rich LD conditions were conducted by incubating the cells for 24 h with DMEM supplemented with CHOL (70000P; Avanti) conjugated to methyl-β-cyclodextrin (at a molar ratio of 1:20; C4555-1G; Sigma-Aldrich) for a final concentration of 250 µM. The LDs were labeled using HCS LipidTOX Deep Red Neutral Lipid Stain (H34477; Invitrogen) or MDH visualization Dye (SM100a; ABCEPTA). Microscopy visualization was conducted on a Carl ZEISS LSM 800 microscope coupled with a polarized light module and a 37°C heating plate for the CHOL-enriched conditions.

Rogers S., Gui L., Kovalenko A., Zoni V., Carpentier M., Ramji K., Ben Mbarek K., Bacle A., Fuchs P., Campomanes P., Reetz E., Speer N.O., Reynolds E., Thiam A.R., Vanni S., Nicastro D, & Henne W.M. (2022). Triglyceride lipolysis triggers liquid crystalline phases in lipid droplets and alters the LD proteome. The Journal of Cell Biology, 221(11), e202205053.

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Regulation of Osteosarcoma Cell Survival

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The Saos2 Tet-on cell line, human osteosarcoma cells with a tetracyclin-inducible gene expression system were purchased from Clontech, Mountain View, CA, USA. The Saos2 cells were modified to stably express Wip1 following a treatment with doxycycline (Sigma–Aldrich, St. Louis, MO, USA, D9891), as described in a previous paper.^{6 (link)} Murine embryonic fibroblasts were isolated according to the standard protocol from wild-type 12-day c57-Bl/6 mice. All cell lines were cultured at 37 °C with 5% CO₂ in a humidified incubator in DMEM high glucose (Dutscher, Brumath, France, L0104-500) with 10% FBS (Pan Biotech, Aidenbach, Germany, 8500-P131704) and Penicillin-Streptomycin-Amphotericin antibiotics (Pan Biotech, P06-07300). To induce Wip1, cells were treated with 1 μg/ml doxycycline 24 h prior to cisplatin (Sigma–Aldrich, P4394) and/or 75nM MK-1775 Wee1 inhibitor treatments (Axon Medchem, Groningen, The Netherlands, 955365-80-7).

Clausse V., Goloudina A.R., Uyanik B., Kochetkova E.Y., Richaud S., Fedorova O.A., Hammann A., Bardou M., Barlev N.A., Garrido C, & Demidov O.N. (2016). Wee1 inhibition potentiates Wip1-dependent p53-negative tumor cell death during chemotherapy. Cell Death & Disease, 7(4), e2195-.

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Cultivation of Colorectal Cancer Cell Lines

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Three human colorectal tumor cell lines were employed: SW-480 (CCL-228), SW-620 (CCL-227) and DLD-1 (CCL-221). All of them were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured in Dulbecco´s modified Eagle´s medium (DMEM-High Glucose, Dominique Dutscher, Bernolsheim, France) with 10% fetal bovine serum (FBS, PAN Biotech, Aidenbach, Germany), 2 mM L-glutamine (Cytiva, Washington, DC, USA) and 1% penicillin/streptomycin (Corning, New York, NY, USA) at 37 C in a humidified incubator (Series II water Jacker, Thermo Scientific, Waltham, MA, USA) with 5% CO₂.

Sánchez-Díez M., Alegría-Aravena N., López-Montes M., Quiroz-Troncoso J., González-Martos R., Menéndez-Rey A., Sánchez-Sánchez J.L., Pastor J.M, & Ramírez-Castillejo C. (2022). Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines. Biomedicines, 10(5), 1083.

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