Methanol (cat no. 1.06018), chloroform (cat no. 34854), acetic acid from Merck, and SOCl3 from Sigma Aldrich were used. Double-distilled water was purchased from a Milli-Q Water System (Millipore, Billerica, MA, USA). Fatty acids standards were obtained from Sigma Aldrich, Neochema (Bodenheim, Germany), and Cayman (Ann Arbor, MI, USA).
Fatty acids standards
Manufactured by Merck Group
Sourced in Germany
Fatty acids standards are reference materials used to identify and quantify the presence of specific fatty acids in various samples. They provide a known concentration of individual fatty acids that can be used to calibrate analytical instruments and validate analytical methods.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Boron trifluoride in methanol, by Merck Group (1 mentions)
2 6 di tert butyl 4 methylphenol bht, by Merck Group (1 mentions)
Milli q water system, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium carbonate, by Merck Group (1 mentions)
4 protocols using fatty acids standards
1
Fatty acids analysis protocol
2
Fatty acid profiling protocol
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Chloroform (cat no. 34854), methanol (cat no. 1.06018) and acetic acid were purchased from Merck. Boron trifluoride in methanol (cat no. B1252), NaCl (cat no. S3014) and 2,6-Di-tert-butyl-4-methylphenol (BHT) (cat no. B1378) were supplied by Sigma-Aldrich. Double-distilled water was obtained from a Milli-Q Water System (Millipore, Billerica, MA, USA). Fatty acids standards were bought form Sigma-Aldrich, Neochema (Germany) and Cayman.
3
Mustard and Rice Bran Oil Extraction
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Mustard seeds were procured from local market then fresh and healthy mustard seeds were stored for oil extraction. Freshly milled rice bran, outer layer of the grain was collected from rice milling industry, Rajshahi, Bangladesh and immediately processed after milling for stabilization. Primarily sieved with 100 mesh size to separate husk and broken rice grain. All solvents and chemicals used were analytical, HPLC and GC grade. Fatty acids standards were purchased from Sigma-Aldrich.
4
Plasma Fatty Acid Analysis in Zellweger Syndrome
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Plasma samples were collected from 52 patients with ZS between 1994 and 2015 in the Laboratory of Biochemistry (Rabta Hospital, Tunis, Tunisia) and 60 from healthy age-and sex-matched controls. Blood was collected into heparinized tubes and maintained at 4 C. The plasma was then separated by centrifugation at 1500g for 10 minutes at 4 C, and then stored at À20 C until analysis. Fatty acids standards and potassium carbonate were purchased from Sigma (St. Louis, MO, USA). Acylchloride was purchased from Aldrich. Acetonitrile, n-hexane, chloroform, methanol, and other chemical reagents were of analytical grade and were of highest purity. The study was performed with the approval of the institutional ethics committee (Rabta University Health Center), and informed consent was obtained from parents.
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