Cas9 2a gfp vector
The Cas9-2A-GFP vector is a plasmid that expresses the Cas9 protein and the green fluorescent protein (GFP) reporter. The Cas9 protein is a key component of the CRISPR-Cas9 gene editing system, which is used for targeted genome modification. The 2A peptide sequence allows for the co-expression of Cas9 and GFP from a single open reading frame, enabling the identification of successfully transfected cells.
2 protocols using cas9 2a gfp vector
RANKL Knockout gRNA Constructs
RANKL Knockout Vector Construction
pNV-RANKL1/KO -5'-CAGGAATTACAACATATCGT-3' (472221110290), pNV-RANKL2/KO -5'-CAGCGATGGTGGATGGCTCA-3' (472221110390), pNV-RANKL3/KO -5'-TTAATAGTGAGATGAGCAAA-3' (472221110490). pmaxGFP (purchased from Lonza, Human MSC Nucleofector® Kit) was used to control transfection efficiency. Plasmids were propagated by transforming Escherichia Coli (Mix&Go! Competent Cells -DH5 Alpha, Zymo Research) using standard procedures.
The plasmids were purified using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) according to the manufacturer's instructions. The plasmid concentration was measured with the NanoDrop One. The plasmids were stored at -20°C.
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