Phosphate-buffered saline (PBS; pH 7.4) was purchased from Thermo Fisher Scientific, Inc. (USA). Albumin from bovine serum (BSA) was purchased from FUJIFILM Wako Pure Chemical Corporation (Japan). Recombinant SARS-CoV-2 spike RBD protein (39 kDa) was purchased from SignalChem Biotech, Inc. (Canada). Anti SARS-CoV-2 Spike RBD polyclonal rabbit IgG antibody was purchased from Sino Biological, Inc. (China). Anti-avian influenza A hemagglutinin rabbit IgG antibody was purchased from Abcam, Inc. (USA). Healthy human serum was purchased from Cosmo Bio Co., Ltd., (Japan). COVID-19 positive and negative human serum samples were purchased from Raybiotech, Inc. (USA). Positive serum samples were collected from patients who were positive for COVID-19 by PCR or antigen testing, and have been inactivated. Polydimethylsiloxane (PDMS; SILPOT 184 W/C) was purchased from Dow Corning Toray Co., Ltd. (Japan). A negative photoresist (SU-8 3050) was purchased from Nippon Kayaku Co., Ltd. (Japan). HiLyte Fluor™ 647 Labeling Kit-NH2 were purchased from Dojindo Molecular Technologies, Inc. (Japan). Two COVID-19 IgM/IgG rapid test LFIA kits, kit A and kit B, were purchased from GenBody Inc. (Korea) and Epigentek (USA), respectively.
Healthy human serum
Manufactured by Cosmo Bio
Sourced in Japan
Healthy human serum is a laboratory-derived product that contains the non-cellular components of blood, including proteins, hormones, and other biomolecules. It is a widely used reference material in various research and diagnostic applications.
Automatically generated - may contain errors
2 protocols using healthy human serum
1
SARS-CoV-2 Spike RBD Protein Binding Assay
2
Fluorescent Labeling of Urinary N-Glycans
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Urinary N-linked glycans were liberated from glycoproteins by hydrazinolysis, according to the method described before [16 (link),17 (link)]. Briefly, the lyophilized samples were subjected to heat at 100 °C for 12 h with anhydrous hydrazine. Repeated evaporations were applied to the samples to remove hydrazine. The released glycans were re-N-acetylated with acetic anhydride in a saturated sodium bicarbonate solution and then passed through a Dowex 50Wx2 (H+) cation exchanger (Dow Chemicals, Midland, MI, USA) to remove sodium ions. The reducing ends of the liberated glycans were tagged with the fluorophore 2-aminopyridine using reductive amination [18 (link),19 (link)]. To analyze human serum N-glycans, 5 μL of pooled healthy human serum was purchased from CosmoBio (Tokyo, Japan). The method used was the same as for the urine samples.
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