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Nanogold fab goat anti rabbit igg h l

Manufactured by Nanoprobes
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Nanogold®-Fab' goat anti-rabbit IgG (H+L) is a laboratory reagent consisting of Fab' fragments of goat anti-rabbit IgG antibodies conjugated to gold nanoparticles. It is designed for use in analytical and imaging techniques that require the detection of rabbit immunoglobulins.

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Lab products found in correlation

Hq silver, by Nanoprobes (2 mentions)

Close spelling variants of this product

Nanogold (33 citations) Nanogold® anti-rabbit IgG (5 citations) Anti-rabbit nanogold (3 citations)

2 protocols using nanogold fab goat anti rabbit igg h l

1

Immunogold Labeling of Duodenal Tissue

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Duodenal tissue collected from 2 day old control and MYO5B KO mice were fixed in 4% buffered formaldehyde solution for 3 days. Thawed cryosections (~100 nm thick) were subjected to indirect immunogold labeling with antisera detecting NHE3, STX3, Rab11 and Rab8a as previously described in detail17 (link). Bound primary antibodies were visualized by Nanogold®-Fab' goat anti-rabbit IgG (H+L) (Nanoprobes, 2004, diluted 1:150) followed by silver enhancement with HQ-Silver® (Nanoprobes, 2012).
Engevik A.C., Kaji I., Engevik M.A., Meyer A.R., Weis V.G., Goldstein A., Hess M.W., Müller T., Koepsell H., Dudeja P.K., Tyska M., Huber L.A., Shub M.D., Ameen N, & Goldenring J.R. (2018). Loss of MYO5B Leads to Reductions in Na+ Absorption with Maintenance of CFTR-dependent Cl− Secretion in Enterocytes.. Gastroenterology, 155(6), 1883-1897.e10.
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2

Tokuyasu-cryosection Immunogold Labeling of Sprouty2

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Tokuyasu-cryosection immunogold labeling (Tokuyasu, 1973 (link)) of cells overexpressing pmNeonGreen-C1-hSprouty2 was performed according to standard protocols (Liou et al., 1996 (link)). Briefly, cells were fixed with 4% (w/v) buffered formaldehyde solution, and thawed ultra-thin cryosections were labeled with mouse anti-Sprouty2 (#60719, Abcam) or rabbit anti-Neongreen (Geley lab) followed by NANOGOLD®-Fab’ goat anti-mouse or NANOGOLD®-Fab’ goat anti-rabbit IgG (H + L; #2002 and #2004, both from Nanoprobes) visualized by silver enhancement (SE) with HQ-Silver® (#2012, Nanoprobes).
Hausott B., Park J.W., Valovka T., Offterdinger M., Hess M.W., Geley S, & Klimaschewski L. (2019). Subcellular Localization of Sprouty2 in Human Glioma Cells. Frontiers in Molecular Neuroscience, 12, 73.
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