Bp recombinase

Manufactured by Thermo Fisher Scientific

Sourced in United States

BP recombinase is a DNA recombination enzyme used in molecular biology. It catalyzes the site-specific recombination between attachment (att) sites, facilitating the transfer of genetic material. The core function of BP recombinase is to enable efficient cloning and manipulation of DNA sequences.

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Lab products found in correlation

Lr recombinase, by Thermo Fisher Scientific (2 mentions) Pdonr207 vector, by Thermo Fisher Scientific (1 mentions)

2 protocols using bp recombinase

Conserved RRSVs gp1 Gene Silencing

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Sequences from RRSVs6gp1 (AF020337), RRSVs9gp1 (GQ329711), and RRSVs10gp1 (U66712) were used to choose conserved 150-nt fragments. First, a BLAST search was performed to evaluate the nucleotide diversity among related sequences. Due to high conservation, 150-nt fragments were randomly chosen in each of the three genes. These fragments (450-nt tandem) were de novo synthetized in a pUC18 vector by Genecust Company (Boynes, France) with att recombination sites for further gateway cloning (Luxembourg). Then, the tandem sequence was first inserted in the gateway cassette of a pDONR207 vector at the BP recombinase site (Invitrogen, Carlsbad, CA, USA). Then, using LR recombinase (Invitrogen), the tandem sequence was transferred into the two gateway cassettes of the pANDA vector in sense and antisense orientations [53 (link)]. The resulting construct, specifically pANDA:siRRSV, was transformed into Agrobacterium strain EHA105 by electroporation.

Lacombe S., Bangratz M., Ta H.A., Nguyen T.D., Gantet P, & Brugidou C. (2021). Optimized RNA-Silencing Strategies for Rice Ragged Stunt Virus Resistance in Rice. Plants, 10(10), 2008.

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Cloning and Transformation of Tea Genes

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The open reading frames (ORFs) of CsMYB1, CsWD40, CsGL3s and CsCPC were amplified from leaf cDNAs prepared from C. sinensis cv Fudingdabai using gene‐specific primers (Supporting Information Table S1), cloned into pDONR221 by BP recombinase (Invitrogen, Life Technologies) and then subcloned into pB2GW7 by LR recombinase (Invitrogen, Life Technologies, Waltham, MA, USA) for Arabidopsis transformation. The ORF of CsMYB1 was amplified and subcloned into pK7WFG2 vector fusion with green fluorescent protein (GFP) protein and driven by 35S promoter for tea plant hairy root transformation.

Li P., Fu J., Xu Y., Shen Y., Zhang Y., Ye Z., Tong W., Zeng X., Yang J., Tang D., Li P., Zuo H., Wu Q., Xia E., Wang S, & Zhao J. (2022). CsMYB1 integrates the regulation of trichome development and catechins biosynthesis in tea plant domestication. The New Phytologist, 234(3), 902-917.

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