Dry dmso

The synthesis of Precursor 1 was accomplished as described by Olberg et al. [27 (link)]. The syntheses of Precursor 2 and reference standard PSMA-1007 were accomplished as previously described [15 (link)]. Tetrabutylammonium hydrogen carbonate (TBAHCO₃)-solution, as well as phosphate-buffered saline (PBS) of GMP grade were provided by ABX advanced biochemical compounds (Radeberg, Germany). Potassium carbonate and 1 M hydrochloric acid (both pro analysis), as well as acetonitrile (for DNA synthesis) were purchased from VWR international (Darmstadt, Germany). Dry DMSO, dry tBuOH and diisopropylethylamine (DIPEA) were acquired from Sigma-Aldrich (Munich, Germany), trifluoroacetic acid (TFA) (for peptide synthesis) from Carl-Roth GmbH (Karlsruhe, Germany) and water for injection (pharmaceutical grade) from Fresenius Kabi (Bad Homburg vor der Höhe, Germany). The HPLC-solvent was mixed from ethanol (VWR international), 0.9% NaCl (BBraun, Melsungen, Germany) and acetic acid (Sigma-Aldrich), all pharmaceutical grade.
Fluorine-18 was produced by irradiation of [¹⁸O]H₂O (Rotem Industries Ltd., Arava, Israel) with 16.5-MeV proton beams by ¹⁸O(p,n)¹⁸F nuclear reaction. Irradiations were performed with the Scanditronix MC32NI cyclotron at the Division of Radiopharmaceutical Chemistry, German Cancer Research Center (DKFZ, Heidelberg, Germany).

Choline Chloride, ChCl (98+%), L-lactic acid (85%–90% in water), were purchased by Alfa Aesar (Massachusetts, United States). l-Arginine (≥98%), acetic acid (≥99.8%), dry DMSO (99.9%) and ethanol (99.8%) were purchased from Sigma-Aldrich (Germany). Choline hydroxide in water solution (47%–50%) was purchased by TCI (Belgium), while 1-butyl-3-methylimidazolium (BMIM) methylcarbonate (98%) methanol solution was purchased from Proionic GmbH (Austria).
Cellulase from Trichoderma reesei (≥1 unit/mg) was purchased from Sigma Aldrich (Germany). Tris buffer (Ultra-pure), sodium hydroxide (98%), glucose (99%), sodium-potassium tartrate (99%), carboxyl methyl cellulose and dinitrosalicylic acid (98%) was purchased from Fisher Scientific (New Hampshire, United States).
Dried Apple fibers (pulp) were supplied by ILVO (Belgium). They were obtained by freeze-drying apple pomace collected after fruit juice production by Konings (Belgium). Then, the dried pomace were de-stoned, de-steal and pre-grinded and sieved (<1 mm). Before use, they were further grounded with blasting milling. These pretreatments allowed to obtain a more homogenous and manageable materials to facilitate the further processing.