Xevo tq s

Manufactured by Waters Corporation

Sourced in United States, United Kingdom

The Xevo TQ-S is a high-performance triple quadrupole mass spectrometer designed for quantitative analysis. It features enhanced sensitivity, resolution, and mass accuracy to provide reliable and precise results for a wide range of applications.

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XEVO TQ-STM (2 citations) Xevo TQ‐S micro MS (4 citations) Xevo-TQ-S mass spectrometry system (2 citations) Xevo TQ-S MS (21 citations) Waters Xevo TQ-S micro (2 citations) Xevo TQ-S mass spectrometer (133 citations) Xevo TQ-S micro MS/MS (2 citations) Xevo TQ-S triple quadrupole MS (5 citations) XEVO TQ-S Mass Spectrometry (3 citations) Xevo TQ-S triple quadruple mass spectrometer (4 citations)

242 protocols using xevo tq s

Quantification of NVP Plasma Levels

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The NVP plasma concentrations were quantified using a tandem quadrupole mass spectrometer (LC/MS/MS) designed for ultra-high performance: Xevo TQ-S (Waters Corporation, Massachusetts) as described by Reddy et al^{[17 ]} modified in our previous publication.^{[15 (link),16 (link)]} Plasma samples were extracted using Bond Elut C18 cartridges, according to the manufacturer’s instructions (Agilent Technologies, California). The eluents were completely evaporated using Thermo Scientific Reacti-Vap evaporators (Thermo Fisher Scientific Inc., California) at 37°C for 30 minutes. This was then reconstituted using 100 µL of equal parts 1:1 acetonitrile and water, vortexed briefly, transferred into 50 mL capped vials, and placed into Xevo TQ-S (Waters Corporation) for quantification. Approximately 1 µL of the sample was injected automatically into the LC/MS/MS instrument and quantified within 5 minutes. NVP plasma concentrations were categorized as <3400 ng/mL (below the therapeutic range), 3400 to 6000 ng/mL (therapeutic range), or >6000 ng/mL (above the therapeutic range).^{[18 (link),19 (link)]}

Omondi E.O., Muigai A., Ngayo M.O., Mungiria J, & Lihana R. (2022). Nevirapine plasma concentration is associated with virologic failure and the emergence of drug-resistant mutations among HIV patients in Kenya: A cross sectional study. Medicine, 101(50), e32346.

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Quantifying Antiretroviral Plasma Levels

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The nevirapine and efavirenz plasma concentrations were measured using a tandem quadrupole mass spectrometer (LC/MS/MS) designed for ultrahigh performance: Xevo TQ-S (Waters Corporation, U.S. A) as described by Reddy et al.¹⁷. Plasma samples were first subjected to a thorough in-house method for the inactivation of the HIV virus. Plasma samples were extracted using Bond Elut C18 cartridges according to the manufacturer’s instructions (Agilent Technologies, USA). The eluents were then completely evaporated using Thermo Scientific™ Reacti-Vap™ Evaporators (Thermo Fisher Scientific Inc., USA) at 37 °C for 30 min. This was then reconstituted using 100μl100 ul of equal parts 1:1 acetonitrile and water, vortexed briefly and transferred into 50 ml capped vials and placed into Xevo TQ-S (Waters Corporation, U.S. A) for quantification. Approximately 1 μl of the samples was injected automatically into the LC/MS/MS instrument and quantified within 5 min.

Ngayo M.O., Oluka M., Bulimo W.D, & Okalebo F.A. (2021). Association between social psychological status and efavirenz and nevirapine plasma concentration among HIV patients in Kenya. Scientific Reports, 11, 22071.

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UPLC-MS/MS Pigment Profiling Protocol

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The samples were analyzed using a Waters UPLC Acquity H Class (Milford, MA, USA, 2010), instrument equipped with a quaternary pump (UPQSM), autosampler injector (UPPDALTC), and eʎ PDA (UPPDALTC) linked to Water Xevo TQ-S micro mass spectrometer detector. Water MassLynx V4.1 software was used for data acquisition and processing. The mass spectrometer detector was operated in positive ESI mode, with capillary voltage at 4.0 kV, 50 V cone voltage, 350 °C desolvation temperature, 150 °C source temperature, and 1 V of collision energy. The pigment extract was separated. The dry pigment extract was dissolved in C₂H₃N/CH₃OH/CH₂O₂ (70:30:0.5, v/v/v). The analysis was carried out using two solvent phases. Solvent phase A CH₂O₂:H₂O (99.9:0.1, v/v) and phase B C₂H₃N:CH₃OH (85:15, v/v), and concentration gradients of these phases were applied: Gradient 1 (A:25%–B:75% (1 min)), Gradient 2 (A:30%–B:70% (1.1 min)), Gradient 3 (A:50%–B:50% (5 min)), Gradient 4 (A:50%–B:50% (10 min)), Gradient 5 (A:95%–B:5% (12.5 min)), Gradient 6 (A:95%–B:5% (13.5 min)), Gradient 7 (A:25%–B:75% (13.75 min)), and Gradient 8 (A:25%–B:75% (18 min)). The mass spectra were recorded in full scan mode over the range m/z 400–800.

Delgado-Garcia M., Gómez-Secundino O., Rodríguez J.A., Mateos-Díaz J.C., Muller-Santos M., Aguilar C.N, & Camacho-Ruiz R.M. (2023). Identification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from Natronococcus sp. and Halorubrum tebenquichense. Microorganisms, 11(9), 2344.

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Quantification of Metabolite Levels

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For detection by Chugai Pharmaceutical Corporate, Ltd, the collected incubating media were extracted by adding acetonitrile/isopropanol (1/1, v/v) containing an internal standard, and were analyzed using AQUITY UPLC (Waters Corp.) and XevoTQ-S (Waters Corp.) and YMC-Triart column (30×2.0 mm I.D., YMC Co., Ltd).
For detection by Taisho Pharmaceutical Co., Ltd, the collected incubating media were mixed with 0.1% formic acid containing acetonitrile with the internal standard and analyzed using a system consisting of a TripleQuad 5500 (AB Sciex, Foster City, USA) and an HPLC system consisting of two LC-30AD series HPLC pumps, a SIL-30AC autosampler, a CTO- 20AC column oven (Shimadzu, Kyoto, Japan) and a Shim-pack XR-ODS 3.0×30 mm, 2.2-µm column (Shimadzu, Kyoto, Japan).
For detection by Toray Industries, Inc., the collected incubating media were extracted by adding acetonitrile containing an internal standard, and were quantified by LC/MS/MS with the liquid chromatography, ACQUITY UPLC I-class system (Waters Corp.) and the mass spectrometer, API-5000 (SCIEX), using CAPCELLPAK C18 MGIII, 50×2.0 mm, 5 µm column (Shiseido Co. Ltd.).

Nakai S., Shibata I., Shitamichi T., Yamaguchi H., Takagi N., Inoue T., Nakagawa T., Kiyokawa J., Wakabayashi S., Miyoshi T., Higashi E., Ishida S., Shiraki N, & Kume S. (2019). Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells. Biology Open, 8(7), bio042192.

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Targeted LC-MS/MS Metabolite Analysis

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Xevo TQ-S (Waters, USA) was utilized for the analysis of all biological samples. Chromatographic separation was conducted on Waters ACQUITY UPLC HSS T3 1.8 μm column (2.1 mm × 100 mm). Column temperature was 45°C and the flow rate was 0.5ml/min. Room temperature was 20 ± 2°C. Phase A consisted of water and 0.1% formic acid (v/v), and phase B consisted of acetonitrile (ACN) 0.1% formic acid (v/v). Column separation was performed by gradient elution: 0-0.5 min, 10% B; 1-2 min, 90% B; 4 min, 10% B.
Electrospray ionization (ESI) and positive ion V mode were used to determine the samples. The main parameters for mass spectrum were set as follows: capillary voltage: 3.5 kv; sample cone: 60 v; source offset: 50 v; source temperature: 150°C; desolvation temperature: 350°C; cone gas flow: 150 L/h; desolvation gas flow: 650 L/h; injection volume: 5 μL.

Yang L., Wang Y., Huang G., Li J., Zhang Z., Ma Z, & Gao Y. (2018). Simultaneous Evaluation of the Influence of Panax ginseng on the Pharmacokinetics of Three Diester Alkaloids after Oral Administration of Aconiti Lateralis Radix in Rats Using UHPLC/QQQ-MS/MS. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 6527549.

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Quantification of Caffeine and Metabolites

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Plasma levels of caffeine, paraxanthine, theophylline and theobromine were quantified by ultra-high performance liquid chromatography (Waters ACQUITY UPLC system) coupled to a tandem quadrupole mass spectrometer (Waters TQD with electrospray ionization or Waters Xevo TQ-S with UniSpray ion source). The method was validated according to international guidelines using a stable isotope-labeled internal standard for each analyte (detailed method available on request). Limit of quantification for all analytes was 5 ng/ml. Plasma samples were collected between December 2007 and August 2018. In order to estimate more precisely the exposure to caffeine and metabolites, and to reduce the heterogeneity due to various time intervals between caffeine intake and blood collections, the sum of caffeine, paraxanthine and theophylline was used in the analyses.

Laaboub N., Gholam M., Sibailly G., Sjaarda J., Delacrétaz A., Dubath C., Grosu C., Piras M., Ansermot N., Crettol S., Vandenberghe F., Grandjean C., Gamma F., Bochud M., von Gunten A., Plessen K.J., Conus P, & Eap C.B. (2021). Associations Between High Plasma Methylxanthine Levels, Sleep Disorders and Polygenic Risk Scores of Caffeine Consumption or Sleep Duration in a Swiss Psychiatric Cohort. Frontiers in Psychiatry, 12, 756403.

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UPLC-MS/MS Method for Compound Quantitation

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UPLC-MS/MS was performed on a Micromass QuattroUltima triple-quadrupole mass spectrometer equipped with an ESI source (Xevo TQS, Waters, United States). Chromatography was performed on a reversed phase UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) from Waters Scientific. Mobile phases were methanol (A) and 4 mmol/l of ammonium acetate (B). The gradient program was as follows: 0–2 min, 10–90% A; 2–4 min, 90% A; 4–4.1 min, 90–10% A; 4.1–6 min, 10% A. The injection volume was 2 μl with a flow rate of 0.3 ml/min and the column temperature was maintained at 40°C. The instrument featured an ESI source and was operated in both positive and negative ion modes. Quantitation was performed using MRM. The source temperature was 150°C, and the desolvation gas temperature was 350°C. The desolvation gas and nebulizer gas (N₂) were set at 650 and 50 l/h, respectively. The flow rate of the collision gas (Ar) was 0.14 ml/min.

Yan Z., Chen W., van der Lee T., Waalwijk C., van Diepeningen A.D., Feng J., Zhang H, & Liu T. (2022). Evaluation of Fusarium Head Blight Resistance in 410 Chinese Wheat Cultivars Selected for Their Climate Conditions and Ecological Niche Using Natural Infection Across Three Distinct Experimental Sites. Frontiers in Plant Science, 13, 916282.

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Quantitative Lipidomic Analysis by UPLC-MS

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Extracted lipids were separated on a Waters I class UPLC equipped with a Waters CSH C18 column (length 100 mm, diameter 2.1mm, particle size 1.7 μm) using a gradient starting with 57% solvent A (50% methanol, 50% water) and 43 % solvent B (99% 2-propanol, 1% methanol), both solvents containing 10 mM ammonium formiate, 0.1% formic acid and 5 μM sodium citrate. For details see Table S1. The UPLC was coupled to an ESI-(QqQ)-tandem mass spectrometer (Waters Xevo TQ-S) for compound detection in +ESI MRM mode. De novo synthesized SLs were discriminated from steady state SLs by incorporation of ¹³C₃,¹⁵N₁-stable isotope labeled L-serine leading to an n+3 mass shift of the corresponding molecular ions and a corresponding n+2/n+3 mass shift of the product ions in MRM mode. For details see Table S2. Samples were injected and processed using MassLynx software, whereas mass spectrometric peaks were quantified according to their peak area ratio with respect to the internal standard using TargetLynx software (both v 4.1 SCN 843) both from Waters Corporation (Manchester, UK). Subsequently, quantification of ceramides, hexosylceramides, and sphingomyelins was adjusted to the length of the acyl-chain and dihydro(hexosyl)ceramide quantification was further adjusted by a factor calculated between the intensities external ceramides and dihydroceramidstandards of the same concentration.

Wu J., Ma S., Sandhoff R., Ming Y., Hotz-Wagenblatt A., Timmerman V., Bonello-Palot N., Schlotter-Weigel B., Auer-Grumbach M., Seeman P., Löscher W.N., Reindl M., Weiss F., Mah E., Weisshaar N., Madi A., Mohr K., Schlimbach T., Velasco Cárdenas R.M., Koeppel J., Grünschläger F., Müller L., Baumeister M., Brügger B., Schmitt M., Wabnitz G., Samstag Y, & Cui G. (2019). Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity, 50(5), 1218-1231.e5.

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HILIC-MS/MS Analysis of Tetrodontoxins

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HILIC-MS/MS analyses of TTXs were carried out using a Waters (Milford, MA, USA) Xevo TQ-S tandem quadrupole mass spectrometer coupled to a Waters Acquity UPLC H-Class.
A Waters ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 × 150 mm column was used. The analytical method was based on a method described by the EU-RL-MB and applied for the interlaboratory validation of HILIC-MS/MS for TTX in mussels, based on the method proposed by Turner [16 (link)] for the validation of PSP and TTX by HILIC-MS/MS. Chromatography conditions are described in Table 2.
The Waters Xevo TQ-S parameters were as follows: the capillary voltage was held at 3 kV, the desolvation temperature was 350 °C, the desolvation gas flow was 650 L/h, the polarity was positive and the MRM transitions are summarized in Table 3. The quantitation and confirmation m/z transitions used were 320.07 > 302.07 and 320.07 > 162.03, respectively. Figure 2 shows a chromatogram obtained following the analysis of TTX in a high-level calibration standard.

Blanco L., Lago J., González V., Paz B., Rambla-Alegre M, & Cabado A.G. (2019). Occurrence of Tetrodotoxin in Bivalves and Gastropods from Harvesting Areas and Other Natural Spaces in Spain. Toxins, 11(6), 331.

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UPLC-MS/MS Assay for Atorvastatin Quantification

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Chromatographic analysis was performed on UPLC-MS/MS system consisting of Acquity UPLC coupled to a tandem mass spectrometry detector XEVO-TQS (Waters, USA) and Acquity UPLC BEH C18 (1.7 μm, 2.1 × 100 mm) column (Waters, USA). The mobile phase consisted of an acetonitrile:5 mM ammonium formate buffer solution (80:20, v/v) at 0.2 mL/min flow rate. The run time was 2.6 min; the sample volume injected was 2.0 μL. The column temperature was set to 40°C and autosampler cooler was set at 8°C. For ATO the mass spectrometer was set in multiple reactions monitoring (MRM) mode in ESI positive ionization mode. Collision energy and cone voltage were 12 and 19 V, respectively. Cone and desolvation gas flow rate were set to 150 and 600 L/min, respectively, using Argon as collision gas at flow rate of 0.15 mL/min. Tandem mass spectrometer was tuned to monitor range m/z 559.25 to m/z 440.30 transition for ATO and range m/z 330.10 to m/z 192.20 transition for the IS (paroxetine), with dwell time of 0.3 s. Multiple reaction monitoring (MRM) data were acquired and analyzed through MassLynx software (Waters, USA).

Patiño-Rodríguez O., Martínez-Medina R.M., Torres-Roque I., Martínez-Delgado M., Mares-García A.S., Escobedo-Moratilla A., Covarrubias-Pinedo A., Arzola-Paniagua A., Herrera-Torres J.L, & Pérez-Urizar J. (2015). Absence of a significant pharmacokinetic interaction between atorvastatin and fenofibrate: a randomized, crossover, study of a fixed-dose formulation in healthy Mexican subjects. Frontiers in Pharmacology, 6, 4.

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