Db 5ms

Manufactured by Shimadzu

Sourced in Japan

The DB-5MS is a type of gas chromatography (GC) column manufactured by Shimadzu. It is a fused silica capillary column with a 5% phenyl-95% dimethylpolysiloxane stationary phase. The DB-5MS column is designed for a wide range of applications in gas chromatography-mass spectrometry (GC-MS) analysis.

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Lab products found in correlation

Qp2010, by Shimadzu (3 mentions) Gcms qp2010 ultra, by Shimadzu (2 mentions) Qp 2010 plus system, by Shimadzu (1 mentions) Qp 2010 system, by Shimadzu (1 mentions) Diallyl disulfide, by Merck Group (1 mentions) Diallyl trisulfide, by Merck Group (1 mentions) Temephos, by Merck Group (1 mentions) Gc 2010 instrument, by Shimadzu (1 mentions) 600 mhz spectrometer, by Bruker (1 mentions) Aoc 20i, by Shimadzu (1 mentions)

Close spelling variants of this product

DB-5MS UI column DB-5MS quartz capillary column DB-5MS column DB-5MS capillary column

8 protocols using db 5ms

GC-MS Analysis of Chemical Composition

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The chemical composition was analyzed with gas-phase chromatography coupled to mass spectrometry (GC/MS) using a Shimadzu QP 2010 Plus System (Shimadzu Corporation, Kyoto, Japan), equipped with silica capillary columns (DB-5MS, length of 30 m, inner diameter of 0.25 mm, and film thickness of 0.25 μm); carrier gas: helium, linear velocity: 36.5 cm·s⁻¹; type of injection: splitless (solution of 2 μL of oil in 500 μL of hexane); injector temperature: 250 °C, temperature range: 60 °C to 250 °C, gradient of 3 °C·min⁻¹; electron impact mass spectrometry: 70 eV; ion source temperature and connection parts: 220 °C. The quantification of each component was performed by peak-area normalization using a flame ionization detector (GC-FID, QP 2010 system, Shimadzu Corporation, Kyoto, Japan) under the same conditions as GC/MS, except for the carrier gas, which was hydrogen.
The components were identified based on the retention index (RI), which was calculated using the retention times of a homologous series of n-alkanes (C8-C40, Sigma-Aldrich, San Luis, Missouri, USA). The pattern of fragmentation observed in the spectra was compared with existing patterns of authentic samples in data system libraries and the literature [52 ].

do Nascimento L.D., Silva S.G., Cascaes M.M., da Costa K.S., Figueiredo P.L., Costa C.M., Andrade E.H, & de Faria L.J. (2021). Drying Effects on Chemical Composition and Antioxidant Activity of Lippia thymoides Essential Oil, a Natural Source of Thymol. Molecules, 26(9), 2621.

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GC-MS Analysis of Organic Compounds

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GC-MS separation and detection was carried out on a Shimadzu GC-MS QP2010 Ultra equipped with a DB-5 ms (30 m × 0.25 mm i.d. × 0.25 μm film thickness, 5% phenyl–95% dimethylpolysiloxane) analytical column from Agilent. The GC-MS instrument was equipped with a split/splitless inlet. Inert splitless liners with a single taper were used. For analysis, 1 μL of sample was injected in splitless mode (220 °C, splitless time 0.5 min, septum purge flow 3 mL/min). The following GC temperature program was used: 70 °C (hold 1 min), 15 °C/min ramp to 135 °C, 2 °C/min ramp to 146 °C, 15 °C/min ramp to 200 °C, 2 °C/min ramp to 210 °C, 15 °C/min ramp to 300 °C. Hold at 300 °C for 5 min. Ultrahigh purity helium (UHP300) was used as a carrier gas maintained at a constant velocity of 40 cm/s. The transfer line to the MS was set to 250 °C, filament source to 250 °C, and quadrupole temperature to 150 °C. The electron ionization (EI) source was operated at 70 eV. Analytes were monitored using selected ion monitoring. For data evaluation, GCMS Postrun analysis software version 4.52 (Shimadzu) was utilized.

Sherman M.E., Smith R.D., Gardner F.M., Goodlett D.R, & Ernst R.K. (2022). A Sensitive GC-MS Method for Quantitation of Lipid A Backbone Components and Terminal Phosphate Modifications. Journal of the American Society for Mass Spectrometry, 33(12), 2301-2309.

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GC-MS Analysis of SCA-CVD Droplets

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The liquid droplet components during the SCA-CVD process were analysed by gas chromatography-mass spectrometry GC–MS (SHIMADZU-QP2010) with a packed column DB-5 MS. Droplets located at the same position of the substrates were collected by breaking off the heating programme at desired times. A mixture of benzimidazole and ferrocene with a molar ratio of 1:1 was used as a reference sample for the GC–-MS test. All samples for GC–MS testing utilized ethanol as a solvent.

Luo L., Hou L., Cui X., Zhan P., He P., Dai C., Li R., Dong J., Zou Y., Liu G., Liu Y, & Zheng J. (2024). Self-condensation-assisted chemical vapour deposition growth of atomically two-dimensional MOF single-crystals. Nature Communications, 15, 3618.

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Gas Chromatography-Mass Spectrometry Analysis

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Gas chromatography–mass spectrometric analyses were performed on a QP2010 system (Shimadzu, Kyoto, Japan) by electronic ionization at 70 eV, employing a chromatographic column (DB-5MS, 30 mm × 0.25 mm, 0.25 μm). Each extract (1 μL) was injected using the splitless injection method. The column temperature was increased from 60 °C to 200 °C at the rate of 6 °C/min (held at 200 °C for 6 minutes), then raised to 220 °C by 5 °C/min, next to 250 °C by 4 °C/min, and finally held at 250 °C for 5 minutes. The injection port was set at 250 °C. Helium gas was used as the carrier at a flow rate of 1 mL/min.
Relative percentage data were obtained from electronic integration of peak areas without the use of a correction factor. The software adopted to handle mass spectra and chromatograms was GC-MS Solutions. Retention indices were determined using retention times of normal alkanes that had been injected after the oil under the same chromatographic conditions mentioned above. The compounds were identified by comparison of their mass spectra with the NIST05.LIB database or with published mass spectra.

Wu C., Wang F., Liu J., Zou Y, & Chen X. (2015). A comparison of volatile fractions obtained from Lonicera macranthoides via different extraction processes: ultrasound, microwave, Soxhlet extraction, hydrodistillation, and cold maceration. Integrative Medicine Research, 4(3), 171-177.

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Characterization of Bio-oil by GC-MS

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The characterization of bio-oil was carried out by gas chromatography coupled to mass spectrometry (GC-MS), after fractionation by classical liquid chromatography, using hexane and dichloromethane eluents.
The analyzes by GC-MS were carried out in a Shimadzu apparatus (QP2010), automatic sampler QP, coupled to the Mass Spectrometer, using a fused silica capillary column DB5-MS (20 m × 0.18 mm in internal diameter, 0.18 μm of phenyl polydimethylsiloxane). Helium was employed as a carrier gas at a flow rate of 0.6 ml min^–1 and the injector (mode split, 1:10) temperature was 280°C. The initial oven temperature was 40°C (5 min hold) and was ramped to 230°C at 5°C min^–1 and 230°C during 10 min. The mass detector was operated under the following conditions: the temperature at 250°C, electron ionization energy, 70 eV; scan range, 40–600 Da; MS interface temperature were maintained at 200°C.
Compounds were identified by comparing mass spectra with the National Institute of Standards and Technology 147 mass spectral library, considering a similarity equal to or greater than 85%. The semiquantitative analysis was done by normalizing the areas of the identified substances.

Mattos C., Andrade J., Salarini Peixoto B., Tavares Moraes N.L., da Cunha Veloso M.C., Alves Romeiro G, & Folly E.D. (2021). Acaricidal Properties of Bio-Oil Derived From Slow Pyrolysis of Crambe abyssinica Fruit Against the Cattle Tick Rhipicephalus microplus (Acari: Ixodidae). Frontiers in Physiology, 12, 768522.

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Characterization of Garlic Oil Compounds

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The organophosphate insecticide temephos, Chinese garlic oil (artificial), diallyl disulfide and diallyl trisulfide were purchased from Sigma Aldrich (Buchs, Switzerland) . All other chemicals used in this study were HPLC grade. 1 H NMR spectra were recorded on a Bruker 600 MHz spectrometer, with tetramethylsilane used as an internal standard. Gas chromatography coupled to mass spectrometry analysis was performed on a Shimadzu GC-2010 instrument employing the following conditions: DB-5 MS (30 m x 25 mm x 25 µm) column; carrier gas: He (1.3 mL/min, in constant linear velocity mode); injector temperature: 220 ºC, in split mode (1:60); and detector temperature: 250 ºC. The temperature was increased at 3 ºC/min from 60 to 210 ºC. Mass spectra were obtained at electron impact of 70 eV and acquired for the 35 to 400 m/z range. The volume injected was 1 μL. The components were identified using GC retention times, calculated by linear interpolation relative to retention times of their main compounds, and by comparison of mass spectra with the NIST (National Institute of Standards and Technology) database.

Dusi R.G., Morais L.d.S., Magalhães N.M.G., Albernaz L.C., Hamilton C.J, & Espindola L.S. (2022). Potential of garlic oil as a biopesticide against all Aedes aegypti life stages. Industrial Crops & Products., 181.

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GC-MS Analysis of Volatile Compounds

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Gas Chromatography coupled to Mass Spectrometry (GC-MS) analysis was done by a Shimadzu QP2010 with an AOC-20i auto-injector using a DB-5MS column (30 m x 0.25 mm, 0.25 mm in thickness). The carrier gas was He at pressure of 57.4 kPa and flow rate of 1.0 mL/min. The split ratio was 1/30, the injector temperature was 250 °C and the injected volume was 0.1 µL. Temperature ranged between 60 and 240 °C, having been increased 3 °C/min. MS were recorded on electron ionization (EI) mode, with ionization energy of 70 eV (scan time: 2 scan/s). The volatile chemical constituents were identified on the basis of their retention indices relative to a homologous series of n-alkanes (C 10 -C 29 ) and by comparing mass spectra with libraries (Wiley 7 and Nist 62) and references of previously published data (Adams 2007).

Estevam E.B.B., Deus I.P.B., Silva V.P.D., Silva E.A.J.D., Alves C.C.F., Alves J.M., Cazal C.M., Magalhães L.G., Pagotti M.C., Esperandim V.R., Souza A.F, & Miranda M.L.D. (2017). In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae). Anais da Academia Brasileira de Ciencias, 89(4).

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Butane Analysis by Headspace GC-MS

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Analyses of butanes were also performed by headspace GC under the following conditions: GC System, column: SUPEL-Q PLOT 30 m x 0.32 mm i.d., SPELCO; carrier: He, 1 mL/min; oven: 60°C to 150°C at 10°C/min then hold 1 min; injection: volatiles interface, 150°C, split 5: 1; detector temperature: 250°C; headspace sampler, loop size: 1 mL; oven temperature: 55°C, loop temperature: 70°C; transfer line temperature: 80°C; vial pressure: 10.2; equilibration time: 15 min. Identification of butanes was performed using GC/MS (Shimadzu GC/MS-QP2010 Ultra) under the following conditions: GC System, column: DB-5MS 30 m x 0.25 mm i.d., J&W; carrier: He, 0.47 mL/min; oven: 40°C (6 min); injection: volatiles interface, 150°C, split 15: 1; detector temperature: 200°C.

Okuda K., Maseda C., Asari M., Isozaki S., Kiya H., Yajima D., Shiono H, & Shimizu K. (2016). Postmortem diffusion of n-butane and i-butane used for anticontagious plugging spray. Legal medicine (Tokyo, Japan), 19.

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