Percp conjugated cd19
PerCP-conjugated CD19 is a fluorescent-labeled antibody that binds to the CD19 antigen, a cell surface marker expressed on B cells. It can be used in flow cytometry applications to identify and quantify B cell populations in biological samples.
4 protocols using percp conjugated cd19
Breg Cell Enumeration by Flow Cytometry
Quantitative analysis of circulating Bregs
Characterization of Circulating B Lymphocytes and Bregs
Flow Cytometric Identification of Regulatory B Cells
One hundred microliters of the blood sample was incubated with 10 µL of CD24, CD38 and CD19 for 20 minutes at 4 C in the dark. Following incubation, red blood cells lysis, washing and analysis by FACS Calibur flow cytometry with CellQuest software (Becton Dickinson Biosciences, USA) were done. An isotype-matched negative control was used for each sample. Forward and side scatter histogram was used to define the lymphocytes population. Then, CD19+ B cells were gated. Then, the expression of CD38 and CD24 on the CD19+B cells was detected. Bregs were identified as CD19+CD24+hiCD38+hi cells (
Flow cytometric detection of regulatory B cells.
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