Na2hpo4

Manufactured by Thermo Fisher Scientific

Sourced in United States, Australia, United Kingdom, Belgium

Na2HPO4, also known as disodium phosphate, is a chemical compound used as a laboratory reagent. It serves as a buffer solution, maintaining a specific pH level in various scientific experiments and applications. As a salt of phosphoric acid, Na2HPO4 is a white, crystalline solid with a variety of uses in the scientific and industrial fields.

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Lab products found in correlation

Close spelling variants of this product

Sodium phosphate dibasic (Na2HPO4) (11 citations) Disodium hydrogen phosphate (Na2HPO4) (4 citations) Na2HPO4.2H2O (4 citations) Na2HPO4·7H2O (3 citations) Anhydrous Na2HPO4 (3 citations) Sodium phosphate dibasic heptahydrate (Na2HPO4•7H2O) (2 citations) Na2HPO4·12 H2O (2 citations) Na2HPO4 anhydrous certified A.C.S. (2 citations)

63 protocols using na2hpo4

Purification of His-Tagged Proteins

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An equal number of cells was lysed in 1 ml of guanidinium lysis buffer
(6M guanidinium-HCl, 0.1M Na₂HPO4 (Sigma)/NaH₂PO4 (Fisher)
pH 8.0, 0.01 M Tris pH 8.0, 5mM imidazole, 10mM β-mercaptoethanol
(Fisher)). Lysates were incubated with 45 μl of Ni2+-NTA-agarose
beads (Thermo Scientific) for 4 hrs at room temperature by end-over-end
rotation. The beads were washed with the following buffers: guanidinium buffer
(6M guanidinium-HCl, 0.1 M Na₂HPO4/NaH₂PO4 pH 8.0, 0.01 M
Tris pH 8.0, 10 mM β-mercaptoethanol); urea pH 8 buffer (8M urea
(Fisher), 0.1 M Na₂HPO4/NaH₂PO4 pH 8.0, 0.01 M Tris pH
8.0, 10 mM β-mercaptoethanol); buffer A (8M urea, 0.1 M
Na₂HPO4/NaH₂PO4 pH 6.3, 0.01 M Tris pH 6.3, 10 mM
β-mercaptoethanol) plus 0.2% Triton-X 100 (Acros); buffer A;
buffer A plus 0.1% Triton X 100. Proteins were eluted with 200 mM
imidazole in 5% SDS (Fisher), 0.15 M Tris pH 6.7, 30% glycerol
(Fisher), 0.72M β-mercaptoethanol. Samples were then subjected to
SDS-PAGE and Western blotting.

Frum R.A., Love I.M., Damle P., Mukhopadhyay N.D., Deb S.P., Deb S, & Grossman S.R. (2016). Constitutive Activation of DNA Damage Checkpoint Signaling Contributes to Mutant p53 Accumulation via Modulation of p53 Ubiquitination. Molecular cancer research : MCR, 14(5), 423-436.

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Enzymatic Assay for Oxidative Stress

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Xanthine (99%) was obtained from Alfa Aesar,
and nitro blue tetrazolium chloride (NBT, 90%) was purchased from
Acros Organics. Xanthine oxidase (0.4–1.0 units/mg protein,
lyophilized powder), 3,3′,5,5′-tetramethylbenzidine
(TMB) (≥99%), and PDADMAC (200–350 kg/mol, 20 wt %)
were purchased from Sigma-Aldrich. NaCH₃COO·3H₂O (≥99.5%) and glacial acetic acid (Ph.Eur./USP), obtained
from VWR, were used to prepare acetate buffer (pH 4.0). Phosphate
buffer (pH 7.0) was prepared using NaH₂PO₄ (99%,
anhydrous) and Na₂HPO₄ (≥99%, GPR RECTAPUR)
purchased from Acros Organics and VWR, respectively. Tris(hydroxymethyl)
aminomethane (TRIS) (AnalaR NORMAPUR), bought from VWR, was used to
prepare TRIS–HCl buffer (pH 9.0). Dimethyl sulfoxide (AnalaR
NORMAPUR), HCl (37 w/w %, AnalaR NORMAPUR), NaOH (≥99.3%, AnalaR
NORMAPUR), H₂O₂ (30% w/w), and NaCl (99.9%)
were obtained from VWR. The glass and plasticware were cleaned using
the Hellmanex III reagent, bought from Hellma. Ultrapure water was
obtained from the Adrona water purification system (Bio-Science Kft)
and was further filtered using polyvinyl difluoride-based syringe
filters (0.1 μm, Millex-VV).

Alsharif N.B., Viczián D., Szcześ A, & Szilagyi I. (2023). Formulation of Antioxidant Composites by Controlled Heteroaggregation of Cerium Oxide and Manganese Oxide Nanozymes. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 127(34), 17201-17212.

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Flexible SWCNT Electrode Fabrication

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CFA (≥98%, Sigma-Aldrich, St. Louis, MO, USA), NADH (98%, Acros Organics, Geel, Belgium), and H₂SO₄ (96%, Merck, Darmstadt, Germany) were used as received. Acetate buffer solution (0.1 M, pH = 4) was prepared with acetic acid (100%, VWR, Radnor, PA, USA) and KOH (Panreac, Barcelona, Spain), whereas 0.1 M phosphate buffer solution (PBS, pH = 7) was prepared from sodium dihydrogen phosphate (NaH₂PO₄·12H₂O, VWR, Radnor, PA, USA) and disodium hydrogen phosphate (Na₂HPO₄, 99%, Acros Organics, Geel, Belgium). All solutions were prepared daily using ultrapure water, obtained from a Millipore DirectQ purification system provided by Millipore (18.2 MΩ·cm resistivity at 25 °C, Burlington, MA, USA). All reagents were of analytical grade and used as received, without further purification.
SWCNTs (Sigma Aldrich, St. Louis, MO, USA), 1,2-dichloroethane (DCE, 99.8%, Acros Organics, Geel, Belgium), polytetrafluoroethylene membranes (Teflon®, filter pore size 0.45 μm, Millipore Omnipore, Burlington, MA, USA), polyethylene terephthalate film (PET, 175 μm thick, HiFi Industrial Film, Stevenage, UK), silver conductive paint (Electrolube, Leicestershire, UK) for the electrical contacts, and Kapton® tape as insulator of the electrical contacts were used to fabricate the flexible SWCNT electrodes.

Heras A., Vulcano F., Garoz-Ruiz J., Porcelli N., Terzi F., Colina A., Seeber R, & Zanardi C. (2019). A Flexible Platform of Electrochemically Functionalized Carbon Nanotubes for NADH Sensors. Sensors (Basel, Switzerland), 19(3), 518.

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Formaldehyde Fixation and Ethanol Dehydration

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The samples were fixed
for 48 h in 3.7% formaldehyde (Biotop/Naxo) in 0.1 M phosphate buffer
(PB) fixation solution, which was replaced after 24 h. One liter of
0.2 M phosphate buffer contained 20.44 g of Na₂HPO₄ and 6.72 g of NaH₂PO₄ (Acros Organics).
For sample dehydration, 99.5% ethanol was used to establish several
dilutions of it in Milli-Q water. Samples were dehydrated in an ascending
ethanol series (40–90%, 10% steps; 96%, 99.5%) at room temperature
(2 h minimum per step; last step overnight followed by replacement
with fresh absolute ethanol).

Priks H., Butelmann T., Illarionov A., Johnston T.G., Fellin C., Tamm T., Nelson A., Kumar R, & Lahtvee P.J. (2020). Physical Confinement Impacts Cellular Phenotypes within Living Materials. ACS Applied Bio Materials, 3(7), 4273-4281.

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Monoclonal Antibody ELISA Protocol

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The monoclonal humanized antibody (OS2966), (5.87 mg/ml solution in PBS + Tween 20 0.1%) was supplied by OncoSynergy (South San Francisco, California, USA). The ELISA (enzyme-linked immunosorbent assay) Costar™ 96-well EIA/RIA plates were purchased from Fisher Scientific (Reinach, Switzerland). Tween 20 was purchased from Applichem Axon lab AG (Baden-Dättwil, Switzerland). HCl, NaCl, KCl, bovine serum albumin (BSA), Fab specific Anti-Human IgG, HRP (horseradish peroxidase) linked Fc specific Anti-Human IgG and 3,3′,5,5′-tetramethylbenzidine (TMB) liquid substrate were supplied by Sigma-Aldrich (Steinheim, Germany). Na₂HPO₄ and KH₂PO₄ were supplied by Acros Organics (Geel, Belgium). All other reagents were at least of reagent grade. All other solvents were HPLC grade (high performance liquid chromatography grade, HiPerSolv Chromatonorm; Darmstadt, Germany). Ultra-pure water (Millipore Milli-Q Gard 1 Purification Pack resistivity > 18MΩcm; Zug, Switzerland) was used to prepare all solutions.

Lapteva M., del Río-Sancho S., Wu E., Carbonell W.S., Böhler C, & Kalia Y.N. (2019). Fractional laser ablation for the targeted cutaneous delivery of an anti-CD29 monoclonal antibody – OS2966. Scientific Reports, 9, 1030.

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Carbohydrate Assimilation in S. glacialimarina

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Carbohydrate assimilation was determined by growing S. glacialimarina TZS-4_T on M-9 minimal media [64.0g Na₂HPO₄, 15.0g KH₂PO₄ (Acros Organics), 2.5g NaCl (Fisher Chemical), and 5.0g NH₄Cl (Fisher Chemical) in 1L ddH₂O (Milli-Q) to make 5× stock] in the presence of a sole carbon source, namely, 200mM glucose (VWR Chemicals), fructose (Fisher Chemical), maltose (Alfa Aesar), or galactose (Fluka), respectively. The cells were grown, as described in section Growth Conditions, in 10ml of M-9 minimal media using starter cultures grown in rMB. Bacterial growth was monitored by measuring OD₆₀₀. M-9 media without any carbon source was used as negative control.

Qasim M.S., Lampi M., Heinonen M.M., Garrido-Zabala B., Bamford D.H., Käkelä R., Roine E, & Sarin L.P. (2021). Cold-Active Shewanella glacialimarina TZS-4T nov. Features a Temperature-Dependent Fatty Acid Profile and Putative Sialic Acid Metabolism. Frontiers in Microbiology, 12, 737641.

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Insulin, Kinase Inhibitors, and Vanadium Compound Preparation

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NovoRapid^® insulin aspart 100 units/mL (Novo Nordisk, Bagsværd, Denmark), sotrastaurin (Item No. 16726, Cayman Chemical, Ann Arbor, MI, USA) and (R)-(+)-etomoxir sodium salt (Cat. No. 4539, Tocris Bioscience) were diluted before use in sterile filtered PBS (137 mM NaCl, 2.7 mM KCl (cat. P9541, Merck KGaA), 10 mM Na₂HPO₄ (cat. 12695147, ACROS Organics™), 1.8 mM KH₂PO₄ (cat. 205920025, ACROS Organics™), pH 7.4). BVT948 (Item No. 16615, Cayman Chemical) and wortmannin (Cat. No. 1232, Tocris Bioscience) were dissolved in dimethyl sulfoxide (DMSO) (cat. D8418, Merck KGaA). BMOV was synthesized using the method previously described by Caravan el al. [48 (link)]

Vilks K., Videja M., Makrecka-Kuka M., Katkevics M., Sevostjanovs E., Grandane A., Dambrova M, & Liepinsh E. (2021). Long-Chain Acylcarnitines Decrease the Phosphorylation of the Insulin Receptor at Tyr1151 Through a PTP1B-Dependent Mechanism. International Journal of Molecular Sciences, 22(12), 6470.

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Yeast Growth Optimization in Minimal Media

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Single colonies were grown in 10 mL YPDA media (Takara) in 50 mL tubes for 24 h. Cultures were washed and inoculated in minimal media at starting optical densities (ODs) of 0.3 or 0.6 according to the experimental design. Minimal media contained 20 g/L glucose (Acros Organics) and 1.7 g/L yeast nitrogen base without amino acids or ammonium sulfate (BD Difco). A 60.5 mM nitrogen concentration in the media was obtained with 4 g/L ammonium sulfate (Acros Organics) or 1.82 g/L urea (Acros Organics). When required, media was buffered at a pH of 7 using 126 mM Na₂HPO₄ (Acros Organics) and 18 mM citric acid (Sigma‐Aldrich) (Prins & Billerbeck, 2021 (link)) and/or supplemented with 5 mM Phe (Sigma‐Aldrich) and/or 5 mM Glu (Sigma‐Aldrich). Cells were grown for 48 h at the required temperature and agitation speed in 50 mL mini‐bioreactor tubes (Corning) in an Innova 44 incubator (New Brunswick Scientific). At the end of the cultivation samples for OD measurements and pCA quantification were taken.

Moreno‐Paz S., van der Hoek R., Eliana E., Martins dos Santos V.A., Schmitz J, & Suarez‐Diez M. (2024). Combinatorial optimization of pathway, process and media for the production of p‐coumaric acid by Saccharomyces cerevisiae. Microbial Biotechnology, 17(3), e14424.

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Preparation of Chlorine Dioxide Solutions

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Concentrated ClO₂ was produced as described previously^{15 (link)} and was stored in the refrigerator at 4 °C. ClO₂ working solutions were prepared in phosphate-buffered saline (PBS;
5 mM Na₂HPO₄ (99%, Acros), 10 mM NaCl (99.5%
(Acros), pH 7.4) immediately prior to each experiment.

Zhong Q., Carratalà A., Shim H., Bachmann V., Jensen J.D, & Kohn T. (2017). Resistance of Echovirus 11 to ClO2 Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness. Environmental Science & Technology, 51(18), 10746-10755.

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Cocrystal Formation via Intermolecular Interactions

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Lidocaine (purity: 97.5%; CAS 137-58-6), l-menthol (purity: 99.7%; CAS 2216-51-5) were purchased from Acros Organics (Morris Plains, NJ, USA), d-menthol (purity: 99%; CAS 15356-70-4) was obtained from Janssen Chimica (Geel, Belgium), and dl-menthol (purity: >98%; CAS 89-78-1) was provided by Alfa Aesar (Karlsruhe, Germany). No further purification steps were required since the cocrystals are directly obtained from the intermolecular interactions of the pure compounds. For the buffer preparation, Na₂HPO₄ and KH₂PO₄ were purchased from Acros Organics, and ultrapure water was prepared via filtration of distilled water through 2 ion-exchange membranes (resistivity = 18.2 MΩ·cm at 25 °C), with a final filtration through a 0.2 µm membrane (Milli-Q^®, Merck, Darmstadt, Germany).

Ma P., Toussaint B., Roberti E.A., Scornet N., Santos Silva A., Castillo Henríquez L., Cadasse M., Négrier P., Massip S., Dufat H., Hammad K., Baraldi C., Gamberini M.C., Richard C., Veesler S., Espeau P., Lee T, & Corvis Y. (2023). New Lidocaine-Based Pharmaceutical Cocrystals: Preparation, Characterization, and Influence of the Racemic vs. Enantiopure Coformer on the Physico-Chemical Properties. Pharmaceutics, 15(4), 1102.

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