Transwell invasion assay was performed using 24-well transwell chambers (0.8 μm; Corning New York, NY, USA) with Matrigel coating. Huh-7 cells (4 x 104) were suspended in 200 μL of serum-free DMEM, and seeded onto the upper chamber. After that, 600 μL of DMEM medium supplemented with 10% FBS was added into the lower chamber. Cells that invaded through the membrane were fixed with 4% paraformaldehyde, stained with 0.2% crystal violet, and photographed using a laser confocal microscope (Olympus CX23 Tokyo, Japan).
24 well transwell chambers 0.8 μm
Manufactured by Corning
Sourced in United States
The 24-well transwell chambers (0.8 μm) are a laboratory equipment used to study cell migration, invasion, and co-culture experiments. The chambers consist of a cell culture insert with a porous membrane that separates the upper and lower compartments, allowing for the exchange of media and dissolved substances between the two chambers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 24 well transwell chambers 0.8 μm
1
Transwell invasion assay of Huh-7 cells
2
Cell Invasion Assay with Transwell
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell invasion was analyzed using 24‐well transwell chambers (0.8 μm; Corning New York, NY, USA) with Matrigel coating. 4 x 104 MiaPaCa-2 and SW1990 cells in 200 μL serum‐free medium were seed onto the upper chamber. After that, the lower chamber contained 600 μL of DMEM medium plus 10% FBS, which acted as a chemoattractant. After 24 h of incubation, the cells that invade from the upper part of the filter to the lower part were fixed with 4% paraformaldehyde, and then stained with 0.2% crystal violet. Subsequently, the invaded cells were photographed using a fluorescence microscope (Olympus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!