Log In Sign up
The largest database of trusted experimental protocols

Dm il light emitting diode

Manufactured by Leica camera
Visit Supplier
Sourced in Germany

The DM IL light-emitting diode is a core component of Leica's laboratory equipment. It serves as a light source for illumination in various microscopy applications. The DM IL provides consistent and reliable illumination to support precise observation and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Geltrex basement membrane matrix, by Thermo Fisher Scientific (2 mentions) Human umbilical vein endothelial cells (huvec), by Thermo Fisher Scientific (2 mentions) Dfc320, by Leica camera (2 mentions) Sk mel 28, by American Type Culture Collection (2 mentions)

2 protocols using dm il light emitting diode

1

Tube Formation Assay for ABCB5+ MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
ABCB5+ MSCs were seeded in stem cell medium in a well of a 24-well plate coated with Geltrex basement membrane matrix (Thermo Fisher Scientific) and incubated for 19–22 h at 37°C and 3.1% CO2. Tube structures were photographed using an inverted microscope (DM IL light-emitting diode; Leica, Wetzlar, Germany) equipped with a digital camera (DFC320; Leica) at ×40 magnification and the photographs qualitatively analyzed per visual inspection for signs of capillary structure formation. Human umbilical vein endothelial cells (Thermo Fisher Scientific) and human skin melanoma cells (SK-MEL-28; American Type Culture Collection, Manassas, VA, USA) served as positive and negative controls, respectively.
Kerstan A., Niebergall-Roth E., Esterlechner J., Schröder H.M., Gasser M., Waaga-Gasser A.M., Goebeler M., Rak K., Schrüfer P., Endres S., Hagenbusch P., Kraft K., Dieter K., Ballikaya S., Stemler N., Sadeghi S., Tappenbeck N., Murphy G.F., Orgill D.P., Frank N.Y., Ganss C., Scharffetter-Kochanek K., Frank M.H, & Kluth M.A. (2020). Ex vivo-expanded highly pure ABCB5+ mesenchymal stromal cells as Good Manufacturing Practice-compliant autologous advanced therapy medicinal product for clinical use: process validation and first in-human data. Cytotherapy, 23(2), 165-175.
+ Open protocol
+ Expand
2

Tube Formation Assay for ABCB5+ MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
ABCB5+ MSCs were seeded in stem cell medium in a well of a 24-well plate coated with Geltrex basement membrane matrix (Thermo Fisher Scientific) and incubated for 19–22 h at 37°C and 3.1% CO2. Tube structures were photographed using an inverted microscope (DM IL light-emitting diode; Leica, Wetzlar, Germany) equipped with a digital camera (DFC320; Leica) at ×40 magnification and the photographs qualitatively analyzed per visual inspection for signs of capillary structure formation. Human umbilical vein endothelial cells (Thermo Fisher Scientific) and human skin melanoma cells (SK-MEL-28; American Type Culture Collection, Manassas, VA, USA) served as positive and negative controls, respectively.
Kerstan A., Niebergall-Roth E., Esterlechner J., Schröder H.M., Gasser M., Waaga-Gasser A.M., Goebeler M., Rak K., Schrüfer P., Endres S., Hagenbusch P., Kraft K., Dieter K., Ballikaya S., Stemler N., Sadeghi S., Tappenbeck N., Murphy G.F., Orgill D.P., Frank N.Y., Ganss C., Scharffetter-Kochanek K., Frank M.H, & Kluth M.A. (2020). Ex vivo-expanded highly pure ABCB5+ mesenchymal stromal cells as Good Manufacturing Practice-compliant autologous advanced therapy medicinal product for clinical use: process validation and first in-human data. Cytotherapy, 23(2), 165-175.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!