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Calcium chloride dihydrate

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Calcium chloride dihydrate is an inorganic chemical compound with the formula CaCl2·2H2O. It is a crystalline solid that is highly soluble in water, forming a slightly acidic solution. Calcium chloride dihydrate is commonly used as a desiccant, a freezing point depressant, and in various industrial and laboratory applications.

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Lab products found in correlation

Sodium chloride (nacl), by Thermo Fisher Scientific (14 mentions) Acetonitrile, by Thermo Fisher Scientific (8 mentions) Magnesium chloride hexahydrate, by Thermo Fisher Scientific (6 mentions) Potassium chloride (kcl), by Merck Group (5 mentions) L ascorbic acid, by Merck Group (4 mentions) Sodium phosphate monobasic monohydrate, by Thermo Fisher Scientific (4 mentions) Ethanol, by Thermo Fisher Scientific (4 mentions) Methanol, by Thermo Fisher Scientific (3 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (3 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Thermo Fisher Scientific (3 mentions) Dopamine hydrochloride da, by Merck Group (2 mentions) Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (2 mentions) Ammonium chloride, by Thermo Fisher Scientific (2 mentions) Chloroform, by Thermo Fisher Scientific (2 mentions) Acetylcholine chloride, by Merck Group (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Acetone, by Thermo Fisher Scientific (2 mentions) N n dimethylformamide (dmf), by Thermo Fisher Scientific (2 mentions) Sodium phosphate, by Thermo Fisher Scientific (2 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)

33 protocols using calcium chloride dihydrate

1

Lactate Oxidase-Based Biosensor Assay

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All chemicals were prepared and stored according to manufacturer recommendations unless otherwise noted. l-lactate oxidase (EC 1.1.3.2) was obtained from USBiological (Salem, MA; Cat: L3000-10) and diluted in distilled, deionized water to make a 1 IU/µl stock solution for storage at 4 °C. Sodium phosphate monobasic monohydrate, sodium phosphate dibasic anhydrous, 1,3 phenylenediamine dihydrochloride (mPD), sodium chloride, calcium chloride dihydrate, and H2O2 (30% in water) were obtained from Thermo Fisher Scientific (Waltham, MA). l-lactate sodium salt, bovine serum albumin (BSA), glutaraldehyde, dopamine hydrochloride (DA), l-ascorbic acid (AA), polyurethane (PU), tetrahydrofuran (THF), and N,N-Dimethylformamide (DMF) were obtained from Sigma-Aldrich Co. (St. Louis, MO). Human Aβ1-42 (Cat: AS-20276) and human scrambled Aβ1-42 (Cat: AS-25383) was obtained from AnaSpec (Fremont, CA) and stored at − 80 °C once reconstituted. CHC (Cat: 5029) and TBOA (Cat: 1223) was obtained from Tocris Bioscience (Minneapolis, MN) and stock solutions were stored at − 20 °C.
Hascup E.R., Sime L.N., Peck M.R, & Hascup K.N. (2022). Amyloid-β42 stimulated hippocampal lactate release is coupled to glutamate uptake. Scientific Reports, 12, 2775.
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2

Synthesis and Characterization of ZnO Nanoparticles

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The principal chemicals and reagents used in this study, Sucrose (C12H22O11) and Zinc Nitrate Hexahydrate (Zn(NO3)2•6H2O) for the synthesis of ZnO NPs were purchased from Sigma-Aldrich Inc (St. Louis, MO). Medium Viscosity Alginic Acid Sodium Salt ((C6H7O7)A(C6H7O7)BNa) (alginate), Calcium Chloride Dihydrate (CaCl2•2H2O), Phosphate Buffered Saline (PBS,10X), Sodium Hydroxide (NaOH) and Terephthalic Acid (C8H6O4) were all obtained from Thermofisher Scientific (Waltham, MA).
Cleetus C.M., Alvarez Primo F., Fregoso G., Lalitha Raveendran N., Noveron J.C., Spencer C.T., Ramana C.V, & Joddar B. (2020). Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy. International Journal of Nanomedicine, 15, 5097-5111.
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3

Comprehensive Protocol for Microbial Analysis

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Magnesium sulfate, triethylammonium acetate buffer, vancomycin, neomycin, amphotericin B, and methylamine solution (40 wt. % in water) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Organic solvents including methanol, chloroform, ethanol, 1-butanol and acetonitrile were all HPLC grade purchased from Thermo Fisher Scientific (Waltham, MA, USA). Calcium chloride dihydrate, ammonium chloride, sodium phosphate dibasic anhydrous, potassium phosphate monobasic, acetic acid (glacial), metronidazole, casamino acids (vitamin-free) and sodium chloride were also purchased from Thermo Fisher Scientific. Ampicillin sodium salt and isopropyl-β-D-thiogalactoside (IPTG) were obtained from Research Products International (RPI; Mt. Prospect, IL, USA). Sep-Pak silica cartridges were purchased from the Waters Corporation (Milford, MA, USA). Kinetex 2.6μ C18 100Å column (50×2.1 mm) and Kinetex C8 100Å column (50×2.1 mm) were purchased from Phenomenex (Torrance, CA, USA).
Dosoky N., Chen Z., Guo Y., McMillan C., Flynn C.R, & Davies S.S. (2019). Two-week administration of engineered Escherichia coli establishes persistent resistance to diet induced obesity even without antibiotic pretreatment.. Applied microbiology and biotechnology, 103(16), 6711-6723.
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4

Rat α9 and α10 Nicotinic Receptor Expression

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Acetylcholine chloride, L-ascorbic acid, bovine serum albumin (BSA), 1,3-ethanediol, mineral oil, potassium chloride, penicillin/streptomycin, and thioanisole, were obtained from Sigma-Aldrich (St. Louis, MO). Acetonitrile, barium chloride dihydrate, calcium chloride dihydrate, HEPES, magnesium chloride hexahydrate, sodium pyruvate, trifluoroacetic acid (TFA), were obtained from Thermo-Fisher Scientific. Iodine, phenol, and potassium ferricyanide were obtained from Arcos Organics (Geel, Blegium). Clones of rat α9 and rat α10 cDNA in pGEMHe and pSGEM, respectively, were generously provided by A. Belen Elgoyhen (Universidad de Buenos Aires, Buenos Aires, Argentina).
Huynh P.N., Harvey P.J., Gajewiak J., Craik D.J, & Michael McIntosh J. (2020). Critical Residue Properties for Potency and Selectivity of α-Conotoxin RgIA Towards α9α10 Nicotinic Acetylcholine Receptors. Biochemical pharmacology, 181, 114124.
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5

Evaluation of Compound Interactions

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All chemicals used in this study were of the highest purity.
Erythromycin, 1,1dimethylbiguanide hydrochloride (metformin), gemfibrozil, p-nitrophenyl phosphate, disodium salt, hexahydrate (pNPP), and calcium chloride dihydrate were purchased from Thermo Scientific. Trimethoprim, diclofenac sodium, gabapentin, and carbamazepine were purchased from Acros Organics. Amoxicillin, albumin from bovine serum (BSA), L-glutathione reduced (GSH), 1-chloro,24-dinitrobenzene (CDNB), potassium chloride, 2-nitrophenyl-β-D-galactopyranoside (ONPG), p-nitrophenyl butyrate (pNPB) were purchased from Sigma Aldrich. L-Leucine-4-nittoanilide, beta-nicotinamide adenine dinucleotide reduced (NADH) were purchased from Alfa Aesar. Sodium pyruvate, magnesium sulfate heptahydrate, and sodium hydrogen carbonate were purchased from Fisher Scientific.
O’Rourke K., Engelmann B., Altenburger R., Rolle-Kampczyk U, & Grintzalis K. (2023). Molecular Responses of Daphnids to Chronic Exposures to Pharmaceuticals. International Journal of Molecular Sciences, 24(4), 4100.
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6

Enzymatic Quantification of L-Glutamate

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l-Glutamate oxidase (EC 1.4.3.11) was obtained from Cosmo Bio Co. (Carlsbad, CA) and diluted in distilled, deionized water to make a 1 U/µl stock solution for storage at 4 °C. Sodium phosphate monobasic monohydrate, sodium phosphate dibasic anhydrous, 1,3 phenylenediamine dihydrochloride (mPD), sodium chloride, calcium chloride dihydrate, and H2O2 (30% in water) were obtained from Thermo Fisher Scientific (Waltham, MA). l-Glutamic acid sodium salt, bovine serum albumin (BSA), glutaraldehyde, KCl, dopamine hydrochloride (DA), and l-ascorbic acid (AA) were obtained from Sigma-Aldrich Co. (St. Louis, MO).
Hascup K.N., Findley C.A., Sime L.N, & Hascup E.R. (2020). Hippocampal alterations in glutamatergic signaling during amyloid progression in AβPP/PS1 mice. Scientific Reports, 10, 14503.
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7

Calcium Chloride Dihydrate and Alginic Acid Protocol

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In this study, calcium chloride dihydrate and alginic acid sodium salt—very low viscosity (pH 5.68 and dynamic viscosity of 37.8 mPa·s) were purchased by Thermo ScientificTM (Waltham, MA, USA). Other used chemicals were p.a. quality.
Savić Gajić I.M., Savić I.M, & Svirčev Z. (2023). Preparation and Characterization of Alginate Hydrogels with High Water-Retaining Capacity. Polymers, 15(12), 2592.
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8

Synthesis of Gold Nanoparticles Using Alginate

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Food grade sodium alginate (SA) was purchased from La Guilde Culinaire. Tetrakis(hydroxymethyl)phosphonium chloride (THPC; 80% in water), chloro(triphenylphosphine)gold(i) (Au(PPh3)Cl), glucono delta-lactone (GDL), sodium borohydride (NaBH4) and p-nitrophenol (4-NP) were all purchased from Sigma-Aldrich (reference numbers 404 861, 254 037, G4750, 452 874 and 241 326 respectively). Calcium carbonate (CaCO3), sodium hydroxide (NaOH) and calcium chloride dihydrate (CaCl2·2H2O) were purchased from Fischer Scientific and are ACS certified (reference numbers C64-500, MSX05901 and C79-500). Toluene, acetone, acetonitrile, benzene and N,N-dimethylformamide (DMF) were also purchased from Fischer Scientific and are ACS graded solvents. All chemicals were used as received without further purification.
Gazil O., Gancheva T., Bilodeau-Calame M., Favis B.D, & Virgilio N. (2020). Controlling the distribution of nanoparticles in hydrogels via interfacial synthesis. Nanoscale Advances, 2(11), 5263-5270.
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9

In Vitro Digestion Protocol

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Ethanol, sodium phosphate, potassium phosphate, acetonitrile, sodium hydroxide, trichloroacetic acid, hydrochloric acid, gallic acid, potassium ferricyanide, ferric chloride and calcium chloride dihydrate were purchased from Fisher Scientific (Pittsburgh, PA, USA). Folin–Ciocalteu’s reagent, potassium chloride, ammonium carbonate, acetic acid glacial and sodium chloride were purchased from Panreac Applichem (Barcelona, Spain). Magnesium chloride hexahydrate and aluminum chloride were from Acros Organics (Pittsburgh, PA, USA), and sodium carbonate anhydrous was purchased from Labkem (Barcelona, Spain).
Human salivary α-amylase (A1031-1KU), porcine pepsin (P6887), porcine pancreatin 4 × USP specifications (P1750), bile bovine (B3883), 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, chrysin and p-coumaric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Kaempferol was purchased from Extrasynthese (Genay, France) and naringenin from Acros Organics (Pittsburgh, PA, USA).
Aylanc V., Tomás A., Russo-Almeida P., Falcão S.I, & Vilas-Boas M. (2021). Assessment of Bioactive Compounds under Simulated Gastrointestinal Digestion of Bee Pollen and Bee Bread: Bioaccessibility and Antioxidant Activity. Antioxidants, 10(5), 651.
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10

Solid-State Ion-Selective Electrode Fabrication

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4-tert-Butylcalix [4 (link)] arene-tetraacetic acid tetraethyl ester (sodium ionophore X), bis(2-ethylhexyl) sebacate (DOS), poly(vinyl chloride) (PVC), tetrahydrofuran (THF), potassium tetrakis(p-chlorophenyl)borate (KTClPB), hydrochloric acid (HCl), and polyvinyl butyral (PVB) were purchased from Sigma Aldrich. Sodium tetrakis-[3,5-bis(trifluoromethyl)phenyl] borate (NaTFPB) was purchased from Alfa Aesar. Sodium chloride was purchased from Acros Organics, and potassium chloride, calcium chloride dihydrate, and magnesium chloride hexahydrate were from Fisher Chemical. Ecoflex 00-30 was purchased from Smooth-On, and carbon black (CB, Vulcan XC 72R) was obtained from FuelCellStore. Noncyanide silver solution (RioGrande) was used to electroplate a thin Ag film for the solid-state RE.
Lim H.R., Kim Y.S., Kwon S., Mahmood M., Kwon Y.T., Lee Y., Lee S.M, & Yeo W.H. (2020). Wireless, Flexible, Ion-Selective Electrode System for Selective and Repeatable Detection of Sodium. Sensors (Basel, Switzerland), 20(11), 3297.
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