Galactose-deficient IgA1 levels in serum were determined by ELISA. Serum Gd-IgA1 levels were detected using KM55 ELISA kit according to the manufacturer’s specifications (IBL, Japan) [20 (link)]. Serum samples were diluted in proportions of 1:50 with EIA buffer and incubation for 60 min with plate lid. Then washing four times with wash buffer, prepared labeled antibody was incubated for 30 min. Plates were washed and added 50 μL TMA solution incubation for 30 min in dark. At last, the color reaction was stopped and the absorbance was measured at 450/625 nm with an EL312 Bio-Kinetics microplate reader (Bio-Tek Instruments, Winooski, VT).
El312 bio kinetics
Manufactured by Agilent Technologies
The EL312 Bio-Kinetics is a laboratory instrument designed for the analysis of biological and kinetic processes. The device provides precise measurements and monitoring capabilities for various applications in the life sciences and biotechnology industries.
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Lab products found in correlation
3 protocols using el312 bio kinetics
1
Quantification of Galactose-Deficient IgA1 by ELISA
2
Quantifying Plasma Soluble FcγRIIIb
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Plasma soluble FcγRIIIb (sFcγRIIIb, Abcam, UK) levels were determined using a commercial ELISA kit in accordance with the manufacturer’s instructions. It was determined by measuring the absorbance of the sample at 450 nm using an EL312 Bio-Kinetics microplate reader (Bio-TekInstruments, Winooski, VT).
3
Quantification of Inflammatory Markers
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The ST6GAL1 levels from plasma and IL-6 and TNF-α levels from cell supernatants were measured using commercial ELISA kits (R&D Systems, USA) according to the manufacturer’s specifications. At last, the absorbance was detected at 450 nm with an EL312 Bio-Kinetics microplate reader (Bio-TekInstruments, Winooski, VT).
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