Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20–30 min. Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), PE anti-myc (Clone: 71D10, Cell Signaling Technology), anti-Muc16t was generated and conjugated in house (9 (link),25 (link)), Alexa Fluor 700 anti-mouse CD45 (Clone: 30-F11, BioLegend), and PE anti-CD3 mouse (clone: UCHT1, BioLegend). Data was collected using a Guava easyCyte HT Flow Cytometer (Luminex, Austin, Texas, USA), a LSRFortessa (BD, Franklin Lakes, NJ, USA), or a Cytoflex LX (Beckman Coulter, Pasadena, CA). Data were analyzed using Flowjo v10.4 software (Flowjo, Ashland, OR, USA).
Apc or fitc anti mouse cd45
Manufactured by Cytek Biosciences
APC or FITC anti-mouse CD45.1 is a fluorescent-labeled antibody that binds to the CD45.1 cell surface marker, which is expressed on various mouse immune cell types. This product can be used to identify and enumerate CD45.1-positive cells in flow cytometry applications.
Automatically generated - may contain errors
3 protocols using apc or fitc anti mouse cd45
1
Flow Cytometry of Mouse Immune Cells
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20 to 30 minutes. Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), PE anti-myc (Clone: 71D10, Cell Signaling Technology), anti-Muc16t was generated and conjugated in house (9, 25 (link)), Alexa Fluor 700 anti-mouse CD45 (Clone: 30-F11, BioLegend), and PE anti-CD3 mouse (clone: UCHT1, BioLegend). Data were collected using a Guava easyCyte HT Flow Cytometer (Luminex), a LSRFortessa (BD Biosciences), or a Cytoflex LX (Beckman Coulter). Data were analyzed using Flowjo v10.4 software (Flowjo).
3
Flow Cytometry Analysis of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell samples were washed and stained in flow buffer (PBS, 1% FBS, 0.1% sodium azide) for 20–30 min. Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), Alexa Fluor 700 anti-mouse CD45 (Clone: 30-F11, BioLegend), PE anti-CD3 mouse monoclonal antibody (clone: UCHT1, BioLegend). Data was collected using a Guava easyCyte HT Flow Cytometer (Luminex, Austin, Texas, USA), a LSRFortessa (BD, Franklin Lakes, NJ, USA), or Cytoflex LX (Beckman Coulter, Pasadena, CA). Data were analyzed using Flowjo v10.4 software (Flowjo, Ashland, OR, USA).
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