YUMM 1.7 cells were harvested using 0.05% trypsin-EDTA solution and after centrifugation were re-suspended in PBS at a final concentration of 1 × 107 cells/mL. Then, 100 μL of cell suspension was subcutaneously injected into the backs of C57Bl/6 mice. After 12 days, tumors reached approximately 500 mm3, and tumor-bearing mice were assigned into the YUMM 1.7 group plus vehicle. Non-tumor-bearing mice were assigned to the control group plus vehicle. On day 12, tail vein injection of fluorescent LP-CuET (1 mg·kg−1) was performed, while vehicle mice were injected with PBS. Tumor-bearing and control mice were anaesthetized with 5% isoflurane, and imaged at 1 h, 12 h, and 24 h following injection using In-Vivo Xtreme (Bruker, Billerica, MA, USA). Ex vivo organ imaging was performed at 1-h and 6-h post IV injection with fluorescent LP-CuET in non-tumor-bearing mice and in vehicle-treated mice. Ex vivo tumor and organ imaging were performed on YUMM 1.7 tumor-bearing mice at 6 h post-IV injection with fluorescent LP-CuET or vehicle. Mice were euthanized, and the liver, kidneys, spleen, heart, lungs, and tumors were excised and imaged with In-Vivo Xtreme (Bruker). Fluorescence intensity was normalized to vehicle mice. Tumor tissues were fixed in 10% formalin, stained with H&E, and imaged using a light microscope.
In vivo xtreme
Manufactured by Bruker
Sourced in Germany, United States
The In-Vivo Xtreme is a fully integrated multimodal imaging system designed for preclinical research. The system combines high-resolution optical and X-ray imaging capabilities in a single platform.
Automatically generated - may contain errors
Lab products found in correlation
Mi se software, by Bruker (5 mentions)
In vivo xtreme imaging system, by Bruker (2 mentions)
α mem, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)
Microct40, by Scanco (2 mentions)
Mi se 721, by Bruker (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Gelfoam, by Pfizer (1 mentions)
Rj athym foxn1nu nu, by Janvier Labs (1 mentions)
D luciferin, by Thermo Fisher Scientific (1 mentions)
Suprane, by Baxter (1 mentions)
Rj nmri foxn1nu nu, by Janvier Labs (1 mentions)
Aperio cs2, by Leica (1 mentions)
Pharmascan 70 16 us, by Bruker (1 mentions)
Male nsg mice, by Charles River Laboratories (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Ivis spectrum in vivo imaging system, by PerkinElmer (1 mentions)
Nxg mice, by Janvier Labs (1 mentions)
Mi se, by Bruker (1 mentions)
Bodipy fl c16, by Thermo Fisher Scientific (1 mentions)
69 protocols using in vivo xtreme
1
In Vivo Fluorescence Imaging of YUMM 1.7 Tumor Xenografts
2
Oral Administration of Fluorescent NPs
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Healthy male C57BL/6 mice aged 6–8 weeks were purchased from the Animal Center of the Chinese Academy of Sciences (Beijing, China). The animal experiments were performed at Experimental Animal Center of School of Pharmacy of Wenzhou Medicine University, and were approved by Experimental Animal Ethics Committee of School of Pharmacy of Wenzhou Medicine University (ID Number: wydw2021-0140). We synthesized Sulfo-cyanine-5 NHS ester labeled LIRA (Cy5-LIRA) and Rhodamine B isothiocyanate-conjugated zein (RITC-zein) as reported [45 (link)], followed by the preparation of double fluorescence-labeled NPs. The mice were fasted overnight with free access to water and randomly divided into four groups. The solutions of LIRA/CA Complex, LIRA/CA@Zein/RLs, LIRA@Zein/RLs, and LIRA/CA@Zein were administrated orally at a dose of 0.4 mg kg−1. After 2, 6, 12, and 24 h, the mice were sacrificed and organs were taken out, washed, and imaged (In Vivo Xtreme, Bruker).
3
Fluorescence Imaging of Blood Filtration
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We fabricated an imaging module for the measurement of fluorescence of blood before and after passing through the filter. A copper plate (3 cm × 3 cm × 0.2 cm) was coated with a thin layer of black epoxy resin, and glass microcapillaries, as used above, were placed on the resin and allowed to dry for 24 h at room temperature (Figure 7 d). During experiments, fluorescence imaging was performed of the blood inside the capillaries using an imaging system (In vivo Xtreme, Bruker Corp., Billerica, MA, USA), with filters appropriate for doxorubicin (excitation 550 nm, emission 600 nm).
4
Quantifying Vaccine Uptake in Lymph Nodes
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Single cell suspensions of lymph nodes were added to black-walled
96-well plates and quantified for AF647-labeled vaccines by performing
epifluorescence imaging (excitation = 650 nm; emission = 700 nm; 0.50 second
exposure) using a Bruker In Vivo Xtreme (Bruker, Billerica, MA); fluorescence
was determined by placing identically-sized regions of interest over each
well.
96-well plates and quantified for AF647-labeled vaccines by performing
epifluorescence imaging (excitation = 650 nm; emission = 700 nm; 0.50 second
exposure) using a Bruker In Vivo Xtreme (Bruker, Billerica, MA); fluorescence
was determined by placing identically-sized regions of interest over each
well.
5
Tracking Encapsulated hMSC Survival in Rats
6
Ectopic Bone Formation in Transgenic Mice
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Eight-week-old Tg6 mice and littermate controls, ΔEpoRE- and wild-type mice were transplanted subcutaneously with 5 μg of BMP2 (#34-8507-85, Ebioscience, CA, USA) soaked into gelatin sponge cubes (7 × 5 × 5 mm) (Gelfoam™, Pfizer, NY, USA). After 4 weeks ectopic bone formation was confirmed using x-ray analysis (In vivo Xtreme, Bruker) and transplants were harvested. After 24 h fixation in 10% neutral buffered formalin, transplants were moved to PBS and analyzed by micro-CT. Following decalcification in 0.05 mol·L–1 EDTA, H&E staining, and TRAP staining procedures were performed.
7
Evaluating RevCAR T Cell Cytotoxicity
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In order to evaluate the cytotoxic potential of RevCAR T cells, co-injection experiments were performed in vivo using six weeks old male NMRI-nude mice (Rj:ATHYM-Foxn1nu/nu) obtained by Janvier Labs. All mice were randomly assigned to experimental groups of five individuals and housed in sterile cages in a pathogen-free facility with a 12 h light/dark cycle. 1*106 LNCaPPSCA+/Luc+ cells were injected subcutaneously in the presence or absence of RevCAR T cells (1*106) and RevTM (102 µg) in a total volume of 100 µl PBS (Gibco™ Cat#10010015). Subsequently, anesthetized mice were i.p. injected with 200 µl D-Luciferin (15 µg/ml in PBS, PerkinElmer Cat#122799) and analyzed for bioluminescence in the planar X-ray (Bruker In-vivo Xtreme) at different time points. Measured luminescence was evaluated using the Bruker MI and Multispectral Software as described previously.43 (link),44 (link) All in vivo studies have been approved by the local ethics committee for animal experiments (Landesdirektion Dresden, 24–9165.40-4/2013, 24.9168.21–4/2004-1) and were performed at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) in appliance with the terms of German regulations of animal welfare.
8
In Vivo Imaging of Subcutaneous Xenografts
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All animal experiments were carried out at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) according to the guidelines of German Regulations for Animal Welfare and have been approved by the Landesdirektion Dresden (24-9165.40-4, 24.9168.21-4/2004-1). Four weeks old female NMRI-Foxn1nu/Foxn1nu mice were purchased from JANVIER LABS (St. Berthevin, France). General anesthesia was induced with 10% (v/v) and maintained with inhalation of 8% (v/v) desflurane (Suprane, Baxter, Germany) in 30/10% (v/v) oxygen/air. Luminescence imaging of the subcutaneous injected cells (exposure times 1 s, 10 s, and 60 s) was performed using a dedicated small animal multimodal imaging system (In-Vivo-Xtreme, Bruker, Germany) 10 min after i.p. injection of 200 µl of D-luciferin (15 mg/ml) (ThermoFisher Scientific). In parallel, an X-ray photograph was taken from the same animals at the same position [39 (link)–41 (link)].
9
Ginsenoside CK Inhibits Lung Metastasis
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As described previously [26 (link), 27 (link)], male BALB/c nude mice (Beijing HFK Bio-Technology, Beijing, China) were injected via the tail vein with A549 cells (1 × 106 cell/mouse). The mice from each group were anesthetized by exposure to 3% isoflurane and intraperitoneally injected with XenoLight D-Luciferin Potassium Salt (150 mg·kg−1) every 7 days for up to 21 days. After 15 min, the photons emitted from the tumor were monitored using a Bruker In Vivo Xtreme (Bruker, Billerica, MA, USA). Nude mice were orally administered 10 mg·kg−1 and 20 mg·kg−1 ginsenoside CK for 3 weeks. Nude mice were sacrificed at the end of the experiment, after which their lungs were removed and fixed in 10% formalin.
The experimental design was in strict accordance with the principles and guidelines recommended by the Committee for the Care and Use of Laboratory Animals of Changchun University of Chinese Medicine (Changchun, China) and approved by the Ethics Committee of Changchun University of Chinese Medicine. Male nude mice were provided by Beijing HFK Bio-Technology (Beijing, China).
The experimental design was in strict accordance with the principles and guidelines recommended by the Committee for the Care and Use of Laboratory Animals of Changchun University of Chinese Medicine (Changchun, China) and approved by the Ethics Committee of Changchun University of Chinese Medicine. Male nude mice were provided by Beijing HFK Bio-Technology (Beijing, China).
10
Optimizing ICG Uptake in VX2 Tumor Cells
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VX2 tumor cells were seeded (5 × 104 per well) in four-well chamber plates (Laboratory Tek II; Thermo Fisher Scientific, Rochester, NH). As cells were cultured to reach 80% confluence, VX2 tumor cells were treated with ICG at (i) concentrations of 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, and 200 μg/mL for 24 h and (ii) a concentration of 100 μg/mL with various incubation times of 0, 1, 2, 4, 8, 12, 24, and 48 h. The non-treated VX2 cell groups served as control. For fluorescent microscopy, the ICG treated cells (ICG cells) were washed with phosphate buffered saline (PBS) twice to remove the free ICG, fixed with 4% paraformaldehyde, and then dried at room temperature. The cells were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA, USA) and imaged with a fluorescent microscope (IX73, Olympus, Tokyo, Japan). For in vitro optical/X-ray imaging, the ICG cells were washed twice with PBS, trypsinized, centrifuged, and then 5 × 106 cells were diluted with 0.2 mL PBS in a 96-well. Subsequently, the cell-containing wells were imaged with an optical/X-ray imaging system (In vivo Xtreme; Bruker, Billerica, MA, USA) at the emission wavelength of 830 nm and excitation wavelength of 760 nm. The fluorescent signal intensity (SI) of cells in different wells was measured and compared.
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