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Rabbit anti somatostatin

Manufactured by Santa Cruz Biotechnology
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Sourced in United Kingdom

Rabbit Anti-Somatostatin is a primary antibody that recognizes the somatostatin protein. Somatostatin is a hormone that inhibits the release of other hormones, such as growth hormone, insulin, and glucagon. The Rabbit Anti-Somatostatin antibody can be used to detect and quantify somatostatin in various biological samples.

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Lab products found in correlation

Rabbit anti c fos, by Santa Cruz Biotechnology (1 mentions) Prolong gold antifade, by Thermo Fisher Scientific (1 mentions) Normal bovine serum, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Fluoromount, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Somatostatin (6 citations) Anti-somatostatin (5 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit anti somatostatin

1

Immunohistochemical Staining of Neuroendocrine Markers

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Immunohistochemical experiments were performed at room temperature in 2 ml microcentrifuge tubes on a rocking plate. All experiments used the same blocking buffer that consists of 0.1% Triton in phosphate-buffered saline and 3% bovine serum albumin (BSA). All sections were mounted using Prolong Gold Antifade (Life Technologies) and kept at 4 °C for short-term storage and −20 °C long-term storage. For SST staining, Rabbit Anti-Somatostatin from Santa Cruz (sc-55565) was used. For JUND staining, Rabbit Anti-JUND from abcam (ab134067) was used. For C-FOS staining, Rabbit Anti-C-FOS from Santa Cruz (sc-52) was used.
Ribeiro E.A., Salery M., Scarpa J.R., Calipari E.S., Hamilton P.J., Ku S.M., Kronman H., Purushothaman I., Juarez B., Heshmati M., Doyle M., Lardner C., Burek D., Strat A., Pirpinias S., Mouzon E., Han M.H., Neve R.L., Bagot R.C., Kasarskis A., Koo J.W, & Nestler E.J. (2018). Transcriptional and physiological adaptations in nucleus accumbens somatostatin interneurons that regulate behavioral responses to cocaine. Nature Communications, 9, 3149.
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2

Immunohistochemical Detection of Interneurons

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Free-floating slices were permeabilized and blocked with PBS containing 0.8% Triton X-100 (Sigma-Aldrich, MO, USA), 5% normal bovine serum (Jackson Immuno Research, PA, USA) and 0.05% sodium azide. Slices were incubated over night with primary antibody rabbit-anti-parvalbumin (1:500, #ab11427, Abcam, UK) or rabbit-anti-somatostatin (1:250, #sc13099, Santa Cruz, CA, USA), followed by 2 hr incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich, MO, USA).
Bitzenhofer S.H., Pöpplau J.A, & Hanganu-Opatz I. (2020). Gamma activity accelerates during prefrontal development. eLife, 9, e56795.
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