Rabbit anti aif antibody

Manufactured by Cell Signaling Technology

Rabbit anti-AIF antibody is a primary antibody raised in rabbit against the Apoptosis-Inducing Factor (AIF) protein. AIF is a mitochondrial flavoprotein that plays a role in caspase-independent apoptosis. This antibody can be used to detect and study the AIF protein in various applications.

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Lab products found in correlation

Mouse anti smac diablo, by BD (2 mentions) Anti phospho jnk antibody, by Cell Signaling Technology (1 mentions) Rabbit anti bax polyclonal antibody, by Cell Signaling Technology (1 mentions) Horseradish peroxidase coupled anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Rabbit anti-AIF Anti-AIF antibody AIF) antibody Anti-AIF

3 protocols using rabbit anti aif antibody

Western Blotting of Apoptosis Markers

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Western blotting was performed, as previously described (Bajt et al., 2000 (link)). The primary antibodies were rabbit anti-JNK and anti-phospho-JNK antibodies (Cell Signaling Technology, Danvers, MA), rabbit anti-Bax polyclonal antibody (Cell Signaling Technology, Danvers, MA), rabbit anti-AIF antibody (Cell Signaling Technology, Danvers, MA), rabbit anti-EndoG (ProSci, Poway, CA) and rabbit anti-nitrotyrosine (Upstate, Lake Placid, NY). A horseradish peroxidase-coupled anti-rabbit IgG (Santa Cruz) was used as secondary antibody.

Du K., McGill M.R., Xie Y., Bajt M.L, & Jaeschke H. (2015). RESVERATROL PREVENTS PROTEIN NITRATION AND RELEASE OF ENDONUCLEASES FROM MITOCHONDRIA DURING ACETAMINOPHEN HEPATOTOXICITY. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 81, 62-70.

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Histological and Western Blot Analysis of Apoptosis

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Formalin-fixed tissue samples embedded in paraffin were cut in 5 μm thickness and stained with hematoxylin and eosin (H&E) for necrosis assessment (Gujral et al., 2002 (link)). Western blotting was performed as described (Bajt et al., 2000 (link)). The primary antibodies were rabbit anti-Bax polyclonal antibody, rabbit anti-AIF antibody (Cell Signaling Technology, Danvers, MA) and mouse anti-Smac/DIABLO (BD Biosciences, San Diego, CA). A horseradish peroxidase-coupled anti-rabbit or anti-mouse IgG (Santa Cruz) was used as secondary antibody.

Du K., McGill M.R., Xie Y, & Jaeschke H. (2015). BENZYL ALCOHOL PROTECTS AGAINST ACETAMINOPHEN HEPATOTOXICITY BY INHIBITING CYTOCHROME P450 ENZYMES BUT CAUSES MITOCHONDRIAL DYSFUNCTION AND CELL DEATH AT HIGHER DOSES. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 86, 253-261.

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Western Blot Analysis of Apoptosis Signaling

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Western blotting was performed as described^{44 (link)} with primary antibodies (1:1000 dilutions): rabbit anti-JNK antibody (Cat. # 9252), rabbit antiphospho-JNK antibody (Cat. # 4668), rabbit anti-Bax polyclonal antibody (Cat. # 2772), rabbit anti-AIF antibody (Cell Signaling Technology, Danvers, MA; Cat # 5318) and mouse anti-Smac/DIABLO (BD Biosciences, San Diego, CA; Cat # 612245). For secondary antibody (1:5000 dilutions), an anti-rabbit or anti-mouse horseradish peroxidase-coupled IgG (Santa Cruz) was used. Protein bands of interest were detected by chemiluminescence (GE Bioscience) on the Licor Odyssey imaging system.

Akakpo J.Y., Ramachandran A., Kandel S.E., Ni H., Kumer S.C., Rumack B.H, & Jaeschke H. (2018). 4-Methylpyrazole Protects against Acetaminophen Hepatotoxicity in Mice and in Primary Human Hepatocytes. Human & experimental toxicology, 37(12), 1310-1322.

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