8453 diode array spectrophotometer

All chemical for synthesis was purchased from Acros Organics, and chemicals were used as received without further purification. All reagents for cell culture and fluorescent confocal microscopy were purchased from Thermo Fisher. UV-Vis spectral analysis were performed by using a Hewlett Packard-8453 diode array spectrophotometer at 25 °C. Fluorescence spectra were measured by using a HORIBA Fluoromax-4 spectrofluorometer. ¹H NMR spectra were obtained on a Varian 300 MHz spectrometer and ¹³C NMR spectra were obtained on a Varian 500 MHz spectrometer in deuterated dimethyl sulfoxide (DMSO-d6). Fluorescence confocal laser microscopy Imaging was performed by using Nikon A1 system with 60x or 100x oil objective.

Electrochemical measurements were recorded using degassed dry MeCN with Bu₄NPF₆ used as conducting salt, I= 0.1 M and the complex concentration was ~1mM. A common three electrode setup was used with a glassy carbon working electrode, a platinum wire as a counter electrode, and Ag/AgNO₃ as reference electrode. All data were referenced vs the Fc^+/0 redox potential. UV-vis spectra were acquired with a Hewlett-Packard 8453 diode array spectrophotometer in anhydrous MeCN. Acid base titration were measured in MeCN solutions with stoichiometric addition of acid or base. Deprotonation of Ru^II-H₀ was done with DBU (pK_aMeCN= 24.34)²² and P₂-t-Bu-phosphazene (pK_aMeCN=33.5).²⁰ Protonation of Ru^IV-H₂ was done with triflic acid (pK_aMeCN= 2.6)^{23 (link)} 2,6-dichloro-anilinium BF₄ (pK_aMeCN= 5.06).²² and 2,6-dimethoxy-pyridine hydrochloride (pK_aMeCN= 7.64).²¹

All reagents and solvents were of commercially available quality except as noted. Acetone was distilled from Drierite under argon atmosphere. [(MeAN)Cu^I](BAr^F) (BAr^F: B(C₆F₅)₄⁻) was synthesized as previously described.^{24 (link)} Sodium phenolates were obtained using a synthetic method similar to previous results.^{23 (link)} All UV–vis measurements were carried out using a Hewlett-Packard 8453 diode array spectrophotometer with a 10 mm quartz cell. The spectrometer was equipped with HP Chemstation software and a Unisoku cryostat for low temperature experiments. ¹H NMR spectra were recorded on a Bruker 400 instrument. Resonance Raman (rR) samples were excited using a Coherent I90C–K Kr⁺ ion laser at 413.1 or 568.2 nm while the sample was immersed in a liquid-nitrogen-cooled (77 K) EPR finger dewar (Wilmad). Power was ~20 mW at the sample for the 413.1 nm line and ~130 mW at 568.2 nm. Data were recorded while rotating the sample to minimize photodecomposition. The spectra were recorded using a Spex 1877 CP triple monochromator with 600, 1200, or 2400 grooves/mm holographic spectrograph grating and detected by an Andor Newton CCD cooled to −80 °C (413.1 nm) or an Andor IDus CCD cooled to −80 °C (568.2 nm). Spectra were calibrated on the energy axis to toluene at room temperature.