Anti elavl1

RIPA lysis buffer (ThermoFisher, MI, USA) was utilized to extract proteins from left ventricular myocardium tissues or cells according to standard protocol. Protein concentration of each sample was measured by using Pierce™ BCA Protein Assay Kit (ThermoFisher, MI, USA). Equal amounts of protein were loaded into SDS–polyacrylamide gels and separated through electrophoresis. Later the proteins were transferred from the gels to PVDF membranes (Sigma-Aldrich, USA). The membranes were blocked with 3% BSA for half an hour at room temperature and then incubated with primary antibodies overnight at 4 °C. On the next day the membranes were washed with TBST 3 times before incubation with specific secondary antibodies for 1 h at room temperature. Signals were detected by using the standard ECL kit. Primary antibodies used in the study were: Anti-ELAVL1 (1:1000; Abcam, USA); Anti-GPx4 (1:5000; Abcam); Anti-ferritin heavy chain (FTH1, 1:1000; Abcam); Anti-Beclin-1 (1:1000; Santa Cruz, USA); Anti-p62 (1:1000; Abcam); Anti-LC3 (1:3000; Abcam); Anti-FOXC1 (1:1000; Abcam); Anti-β-actin (1: 1000; Santa Cruz).