Immunohistochemical staining for Ki-67 (Cat # sc-7907, Santa Cruz Biotechnology, Santa Cruz, CA), PCNA (sc-15402; Santa Cruz Biotechnology, Santa Cruz, CA), α-amylase (Cat # 3796, Cell Signaling Technology (Beverly, MA) and p-EGFR (Cat # ab40815; Abcam, Cambridge, UK) was performed on human PC xenograft tissue samples or mouse pancreatic samples, as previously described (27 (link)).
α amylase
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
α-amylase is an enzyme that catalyzes the hydrolysis of α-1,4-glycosidic bonds in starch, glycogen, and related polysaccharides. It plays a key role in the breakdown of complex carbohydrates into smaller, more easily digestible units.
Automatically generated - may contain errors
Lab products found in correlation
Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions)
P egfr, by Abcam (1 mentions)
Bromodeoxyuridine (brdu), by Abcam (1 mentions)
Mouse met, by R&D Systems (1 mentions)
β actin, by Merck Group (1 mentions)
Insulin, by Cell Signaling Technology (1 mentions)
Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions)
Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Insulin antibody, by Cell Signaling Technology (1 mentions)
Ck19 antibody, by Abcam (1 mentions)
3 protocols using α amylase
1
Immunohistochemical Analysis of Cancer Markers
2
Immunohistochemical and Western Blot Analysis
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Antibodies against the following were used for IHC: BrdU (1:1000; abcam), insulin (1:1000; Cell Signaling), Ki67, a cellular marker for proliferation (1:3000; abcam), Ly6G, a marker for neutrophils (1:80; abcam), isolated macrophages of mouse origin (1:100, Santa Cruz, sc-101447), mouse MET (1.8 μg/ml; R&D), human MET (1 μg/ml; R&D). The following dilutions were used for western analysis: α-amylase (1:200000; Cell signaling), β-actin (1:10,000; Sigma) and mouse MET (0.2 μg/ml; R&D).
3
Immunofluorescence Staining of Frozen Tissue Sections
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Serial frozen sections (5–7 μm) were rehydrated in PBS, permeabilized with 0.5% Triton X solution, blocked with 10% BSA, and probed with either αSMA antibody (Novus Biologicals, NB500-631, 1:200), CK19 antibody (Abcam, ab52625, 1:200), insulin antibody (Cell Signaling Technology, 4590S, 1:200), or α-Amylase (Cell Signaling Technology, 3796S, 1:200) overnight at 4°C. Epitope retrieval was performed using 1× sodium citrate buffer, followed by Triton X permeabilization. Subsequently, slides were stained with the secondary antibody Alexa Fluor 594 goat anti-rabbit IgG (Life Technologies, A11037, 1:1,000). Slides were mounted with Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200), and coverslips were sealed.
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