Aqua ld stain

Manufactured by Thermo Fisher Scientific

Aqua LD stain is a laboratory reagent used for DNA and RNA detection and quantification. It is a fluorescent dye that binds to nucleic acids, allowing for their visualization and measurement. The core function of Aqua LD stain is to provide a reliable and sensitive method for the detection and quantification of DNA and RNA in various analytical applications.

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Lab products found in correlation

Ova specific dextramer, by Immudex (2 mentions) Lsrfortessa, by BD (2 mentions) Anti cd16 32, by Thermo Fisher Scientific (1 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Cd11b, by BD (1 mentions) Mhcii, by BD (1 mentions) Facscalibur, by BD (1 mentions)

2 protocols using aqua ld stain

Murine blood cell phenotyping by flow cytometry

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Peripheral blood cells were collected from the facial vein into microfuge tubes containing 10 μL of 0.5M EDTA. Erythrocyte were lysed using RBC lysis buffer (eBioscience, San Diego, CA). Cells were washed in FACS buffer (PBS, 0.1% heat-inactivated fetal calf serum, 0.01% sodium azide), stained for extracellular markers, and fixed in 4% paraformaldehyde. OVA-specific dextramer was purchased from Immudex (Copenhagen, Denmark). Aqua LD stain (Life Technologies, Grand Island, NY) was employed for live/dead differentiation. Antibodies were obtained from eBioscience, Biolegend (San Diego, CA), or BD Biosciences (San Jose, CA): TCRβ (Clone H57-597), CD8α (Clone 53–6.7), CD44 (Clone IM7), CD11b (Clone M1/70), and pro-IL-1b (Clone NJTEN3), and CD86 (Clone GL1). FCR blocking was performed using anti-CD16/32 (Clone 93, Ebioscience). Data was captured using an LSR-Fortessa (BD Biosciences) and analyzed using FlowJo software (Treestar, Ashland, OR).

Hu J.C., Greene C.J., King-Lyons N.D, & Connell T.D. (2015). The Divergent CD8+ T Cell Adjuvant Properties of LT-IIb and LT-IIc, Two Type II Heat-Labile Enterotoxins, Are Conferred by Their Ganglioside-Binding B Subunits. PLoS ONE, 10(11), e0142942.

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Multiparametric Flow Cytometry Analysis

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Cells were suspended in FACS buffer (PBS, 0.1% heat-inactivated fetal calf serum, 0.01% sodium azide), stained for extracellular markers, and fixed in 4% paraformaldehyde. Ab were obtained from eBioscience (San Diego, CA), Biolegend (San Diego, CA), or BD Biosciences (San Jose, CA): TCRβ (Clone H57-597), CD8α (Clone 53-6.7), CD11c (Clone N418), MHC-II (Clone M5/T14.15.2), CD80 (Clone 16-10A1), CD86 (Clone GL1), CD40 (Clone 1C10). OVA-specific dextramer was purchased from Immudex (Copenhagen, Denmark). Aqua LD stain (Life Technologies, Grand Island, NY) was employed for live dead differentiation. Data was captured using FACSCalibur (BD Biosciences), LSR-II (BD Biosciences), or LSR-Fortessa (BD Biosciences) and analyzed using FlowJo (Treestar, Ashland, OR).

Hu J.C., Mathias-Santos C., Greene C.J., King-Lyons N.D., Rodrigues J.F., Hajishengallis G., Ferreira L.C, & Connell T.D. (2014). Intradermal Administration of the Type II Heat-Labile Enterotoxins LT-IIb and LT-IIc of Enterotoxigenic Escherichia coli Enhances Humoral and CD8+ T Cell Immunity to a Co-Administered Antigen. PLoS ONE, 9(12), e113978.

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