T cells isolated from tissue suspensions as above were purified using a human T cell enrichment kit (STEMCELL Technologies, Vancouver, BC). A portion of these cells was subjected to CD25 depletion using BD IMag CD25 Magnetic Particles (BD Biosciences), with the CD25-depleted population being collected in the column flow-through. Purified T cells (unfractionated and CD25-depleted) were labeled with 2.5μM CFSE for 15min. at 37 °C, washed with media+FBS to quench excess CFSE, and plated in 96-well round bottom plates (100,000 cells per well) +/− anti-CD2/CD3/CD28-coupled beads (one bead/cell; T cell activation/expansion kit, Miltenyi Biotech) for four days at 37 °C incubator with 5% CO2, after which cells were harvested and analyzed by flow cytometry.
One bead cell t cell activation expansion kit
Manufactured by Miltenyi Biotec
The one bead/cell; T cell activation/expansion kit is a laboratory equipment product designed to activate and expand T cells. It facilitates the stimulation and proliferation of T cells in a controlled in vitro environment.
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Lab products found in correlation
2 protocols using one bead cell t cell activation expansion kit
1
T Cell Isolation and Activation Assay
2
T Cell Isolation and Activation Assay
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