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Fluozin 1

Manufactured by Thermo Fisher Scientific
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FluoZin-1 is a fluorescent indicator for zinc ions (Zn2+). It exhibits an increase in fluorescence intensity upon binding to Zn2+. This product can be used for the detection and measurement of intracellular zinc levels in biological samples.

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Lab products found in correlation

Fluozin 3, by Thermo Fisher Scientific (2 mentions) Fura 2, by Thermo Fisher Scientific (2 mentions) Calcium green 5n, by Thermo Fisher Scientific (2 mentions) Fluo 5n, by Thermo Fisher Scientific (2 mentions) Fluozin 2, by Thermo Fisher Scientific (2 mentions) Indo 1, by Thermo Fisher Scientific (2 mentions) Leadmium green, by Thermo Fisher Scientific (2 mentions) Mag fura 2, by Thermo Fisher Scientific (2 mentions) Newport green dcf, by Thermo Fisher Scientific (2 mentions) Rhod 5n, by Thermo Fisher Scientific (2 mentions)

3 protocols using fluozin 1

1

Cell Culture Media and Fluorescent Probes

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Cell culture media, N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), and fluorescent probes (Calcium Green-5N, Fluo-5N, FluoZin-1, FluoZin-2, FluoZin-3, fura-2, indo-1, Leadmium Green, mag-fura-2, Newport Green DCF, and Rhod-5N) were purchased from Thermo Fisher Scientific. fura-2FF was purchased from TEF Labs. Fetal bovine serum was purchased from Midwest Scientific. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
Malaiyandi L.M., Sharthiya H., Barakat A.N., Edwards J.R, & Dineley K.E. (2019). Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 32(6), 951-964.
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2

Zinc-Dependent FluoZin-1 Fluorescence Assay

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Reactions were performed in a final volume of 100μl containing 50 μM FluoZin™-1 (ThermoFisher, F24180) and 50 μM ZnCl2 in20 mM Tris pH 7.5, 5% DMSO. THL was added at the indicated concentrations in the presence and absence of 2.5 mM β-mercaptoethanol. FluoZin™-1 fluorescence (Ex/Em: 495/515nm) was measured after 5 mins of incubation in black 96-well plates using an EnSpire multilabel plate reader with a monochromator.
Lauinger L., Li J., Shostak A., Cemel I.A., Ha N., Zhang Y., Merkl P.E., Obermeyer S., Stankovic-Valentin N., Schafmeier T., Wever W.J., Bowers A.A., Carter K.P., Palmer A.E., Tschochner H., Melchior F., Deshaies R.J., Brunner M, & Diernfellner A. (2017). Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nature chemical biology, 13(7), 709-714.
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3

Cell Culture Media and Fluorescent Probes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture media, N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), and fluorescent probes (Calcium Green-5N, Fluo-5N, FluoZin-1, FluoZin-2, FluoZin-3, fura-2, indo-1, Leadmium Green, mag-fura-2, Newport Green DCF, and Rhod-5N) were purchased from Thermo Fisher Scientific. fura-2FF was purchased from TEF Labs. Fetal bovine serum was purchased from Midwest Scientific. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
Malaiyandi L.M., Sharthiya H., Barakat A.N., Edwards J.R, & Dineley K.E. (2019). Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 32(6), 951-964.
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