Streptozocin stz

Scutellarin (purity 98%) was purchased from Honghe Qianshan Bioengineering Co., Ltd. (Honghe, China). Streptozocin (STZ) and rosiglitazone were obtained from Multi Sciences (Lianke) Biotech, Co., Ltd. (Hangzhou, China). Antibodies to detect β-actin, procaspase-3, procaspase-8, procaspase-9, cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, Bax, Cyt-C, Bcl-2, Beclin-1, and LC3-II were ordered from Santa Cruz Biotechnology. The procaspase-12 polyclonal antibody was ordered from Abcam Company. Adult male Sprague-Dawley (SD) rats (180 to 200 g) were obtained from the Experimental Animal Center of Kunming Medical University, China. Two weeks after the adaptive feeding of the rats, they were randomly divided into control group, model group, SCU low-dose (100 mg/kg/d), high-dose (200 mg/kg/d) treatment group, and rosiglitazone (5 mg/kg/d) positive control group (each group of ten). The model group was given a high-sugar and high-fat diet for 8 weeks and injected with STZ by 35 mg/kg intraperitoneally, while the control group was given a normal diet. The SCU groups were administered orally for 8 weeks [12 (link)].

The MGE IIe (purity 98%) was purchased from Honghe Qianshan Bioengineering Co., Ltd. (Honghe, China). Streptozocin (STZ) and rosiglitazone were obtained from Multi Sciences Biotech Co. Ltd. (Hangzhou, China). Antibodies against ß-actin, caspase-3, -8, -9, -12, Bax, Cyt-C, and Bcl-2 were obtained from Cell Signaling Technology (Danvers, MA, United States). ELISA kits for the detection of IL-1, IL-6, TNF-α, CK-MB, HDL, and LDL were purchased from Thermo Fisher Scientific (Waltham, MA, United States). The mRNA extraction kit, reverse transcription kit, PCR mixture, and Masson staining kit were purchased from Tiangen Biochemical Technology Co., Ltd. (Beijing, China). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies (Kumamoto, Japan).