Ab307718

NK (purity, >98%) was acquired from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China) (495–31–8) (250 mg administered). Lipopolysaccharide (LPS, 297–473–0) was acquired from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against α-SMA (bs-10196R) and collagen type I (collagen-I) (bs-0578R) were obtained from Bioss (Woburn, MA, USA). Antibodies against PPARα (sc-398394), SREBP-1 (sc-365513), caspase-1 (sc-56036), IL-23 (sc-271279), ASC (sc-514414), Lipin-1 (sc-376874), Nur77 (sc-365113), NLRP3 (ab4207), P2X7r (sc-514962), and IL-1R1 (sc-393998) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against NLRP3 (ab4207), Lipin-1 (ab181389), P2X7r (ab307718), MPO (ab208670), and GAPDH (ab8245) were obtained from Abcam (Cambridge, MA, USA). Csn-B (a Nur77 agonist, 321661–62–5) was purchased from Beyotime Biotechnology (Shanghai, CN, USA). rNur77 (ab152448) was purchased from Abcam (Cambridge, MA, USA).

After the cells were immobilized in 10% paraformaldehyde; permeabilized with 0.1% Triton X-100; blocked with 10% goat serum; and incubated overnight at 4C with primary antibodies of PPARα (santa cruz, sc-398394), Nur77 (santa cruz, sc-365113), IL-23 (santa cruz, sc-271279), NLRP3 (abcam, ab4207), Lipin-1 (abcam, ab181389), P2X7r (abcam, ab307718), and MPO (abcam, ab208670), the secondary antibody was added for cell incubation the next day. Later, DAPI was added to the cells, which were then observed with a fluorescence microscope.