Whole blood (150μl) was immunolabeled for 15 minutes at room temperature using the following antibodies [with catalog numbers; concentration]: 5μl for each of the following APC-Cy7-CD4 [557871; 0.2mg/ml], PerCP-Cy5.5-CD8 [560662; 0.05mg/ml], Alexa Fluor 700-CD14 [557923; 0.2mg/ml], BV605-CD25 [562660; 0.025mg/ml], PE-Cy7-CD196 [560620; 0.2mg/ml], BV421-CD194 [562579; 0.2mg/ml], PE-CF594-CD183 [562451; 0.2mg/ml], PE-CD127 [557938; 0.2mg/ml], V500-CD19 [561121; 0.2mg/ml] and 20μl of the following: FITC-CD3 [555339; 0.05mg/ml], and APC-CD56 [555518; 0.025mg/ml] (all from BD Biosciences) and 35μl buffer solution (BD Canada). After the immunolabelling was completed, red blood cells were lysed (0.06M NH4Cl, 4mM KHCO3, 0.052mM EDTA), and peripheral blood mononuclear cells were washed with PBS, PFA-fixed, and analyzed using a flow cytometer (LSR Fortessa, BD Biosciences). A minimum of 300,000 events were acquired per sample. Data analysis was performed using FlowJo (Tree Star Inc).
Pe cf594 cd183
Manufactured by BD
Sourced in Canada
PE-CF594-CD183 is a fluorochrome-conjugated antibody used in flow cytometry applications to detect the expression of the CD183 protein. It is a tool for researchers to identify and quantify cells expressing the CD183 marker.
Automatically generated - may contain errors
Lab products found in correlation
Cd127 pe, by BD (2 mentions)
Cd3 fitc, by BD (2 mentions)
Cd56 apc, by BD (2 mentions)
Cd4 apc cy7, by BD (2 mentions)
Cd25 bv605, by BD (2 mentions)
Cd14 alexa fluor 700, by BD (2 mentions)
Cd19 v500, by BD (2 mentions)
Percp cy5.5 cd8, by BD (2 mentions)
Pe cy7 cd196, by BD (2 mentions)
Bv421 cd194, by BD (2 mentions)
Lsrfortessa, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
2 protocols using pe cf594 cd183
1
Comprehensive Immunophenotyping of Whole Blood
2
Multiparametric Flow Cytometry Immunophenotyping
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Whole blood (100 μl) was immunolabeled for 15 minutes at room temperature using the following antibodies: FITC-CD3, APC-Cy7-CD4, PerCP-Cy5.5-CD8, PE-CD127, V500-CD19, PE-CF594-CD183, PE-Cy7-CD196, BV605-CD25, BV421-CD194, Alexa Fluor 700-CD14, and APC-CD56 (all from BD Biosciences, Mississauga, Canada). Red blood cells were lysed (0.06 M NH 4 Cl, 4 mM KHCO 3 , 0.052 mM EDTA), and peripheral blood mononuclear cells washed with PBS, PFA-fixed, and analyzed using a flow cytometer (LSRFortessa, BD Biosciences, Ontario, Canada). A minimum of 300,000 events were acquired per sample. Data analysis was performed using FlowJo (Tree Star Inc, Ashland, OR) (gating strategy is shown in Supplemental Figure 1).
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