Vegfr 2 fitc

Manufactured by BD

Sourced in United States

VEGFR-2-FITC is a fluorescently-labeled antibody that binds to the vascular endothelial growth factor receptor 2 (VEGFR-2). VEGFR-2 is a tyrosine kinase receptor that plays a key role in the regulation of angiogenesis, the process of new blood vessel formation.

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3 protocols using vegfr 2 fitc

Decidual Cell Characterization by FACS

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The decidual unsorted cells (dUCs; including dCD34⁺ and dCD34^‐ cells) were collected and labelled with the following antibodies for dual staining: CD34‐FITC (BD, Franklin Lakes, NJ, USA)/ c‐kit‐PE (BD) for 30 minutes at 4°C. The dCD34⁺ cells were stained with CD34‐FITC (BD), c‐kit‐PE (BD), CD90‐FITC (BioLegend, San Diego, CA, USA), CD105‐APC (BioLegend), CD31‐FITC (BD), VEGFR‐2‐FITC (BD), VE‐cadherin‐FITC (BD), HLA‐ABC‐FITC (Abcam) and HLA‐DR‐FITC (Abcam). Then, the cells were washed three times with cold PBS before being centrifuged at 1000 rpm for 5 minutes. Immunoreactivity of the cell surface antibody markers was assayed by fluorescence‐activated cell sorting (FACS; BD).

Bai L., Sun L., Chen W., Liu K., Zhang C., Wang F., Zhang G., Huang Y., Li J., Gao Y., Sun X., Liu W., Du G., Li R., Huang M, & Tian H. (2020). Evidence for the existence of CD34+ angiogenic stem cells in human first‐trimester decidua and their therapeutic for ischaemic heart disease. Journal of Cellular and Molecular Medicine, 24(20), 11837-11848.

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Isolating and Characterizing Lymphocytes

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Blood samples were collected in 0.1 M EDTA-2Na to prevent the blood clotting from the tail vein. After erythrocyte lysis, the viable lymphocyte population in peripheral whole blood was incubated with VEGFR-2-FITC (BD Pharmingen), Sca-1-PerCP-Cy5.5 (eBiosciences), and CXCR4-APC (eBiosciences) and then fixed in 1% paraformaldehyde. Flow cytometry analysis was performed with a FACS Calibur or FACS Canto II (BD Biosciences) using gates to exclude dead cells, debris, and platelets. Isotype control (eBioscience) antibodies were used to exclude false-positive cells.

Chen H., Wang S., Zhang J., Ren X., Zhang R., Shi W., Lv Y., Zhou Y., Yan X., Chen L., He L., Zhang B., Nan X., Yue W., Li Y, & Pei X. (2014). A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment. Scientific Reports, 4, 6222.

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Quantifying Endothelial Progenitor Cells

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Primary mononuclear cells and cultured EPCs were analyzed by flow cytometry as previously described [3 (link), 4 ]. Viable cell populations were analyzed for CD11b-PE, CD34-APC, VEGFR2-FITC (BD Biosciences), and AC133-PE-Cy7 (Miltenyi, Auburn, CA). VEGFR2+/CD11b−/CD34+/AC133+ cells were defined as EPCs. Isotype-identical antibodies (BD Biosciences) served as controls in each experiment. Samples were run on a LSRI analyzer (BD Biosciences) with a minimum of 200,000 events for mononuclear cells and 50,000 events for cultured EPCs. Data were analyzed using FLOWJO software (Tree Star, Ashland, OR).

Chang E., Paterno J., Duscher D., Maan Z.N., Chen J.S., Januszyk M., Rodrigues M., Rennert R.C., Bishop S., Whitmore A.J., Whittam A.J., Longaker M.T, & Gurtner G.C. (2015). Exercise Induces SDF-1 Mediated Release of Endothelial Progenitor Cells with Increased Vasculogenic Function. Plastic and reconstructive surgery, 135(2), 340e-350e.

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