High resolution scmos camera

Retinal sections were imaged with a Zeiss LSM710 confocal microscope. Images of retinal sections were taken in the ventral or dorsal area of the eye. Outer nuclear layer (ONL) thickness was measured 250 μm from the optic nerve using the Fiji software. Rod bipolar cell, horizontal cell, and cone bipolar cell dendrite areas close to the optic nerve were quantified as pixels in the outer plexiform layer using Fiji. Flatmounts were imaged as Z-Stack with a custom-made VisiScope CSU-X1 confocal system equipped with a high-resolution sCMOS camera (Visitron Systems) or with a Keyence BZ-X800 microscope using the sectioning tool. Relative vessel area was quantified using the AngioTool software.¹⁷ (link) Acellular capillaries were manually counted in brightfield images with equal size using Fiji. Retinal pigment epithelium cell morphology parameters were measured in images with equal size using CellProfiler 4.0.7. Nuclei per RPE cell were manually counted. All data were plotted using GraphPad Prism 9.3. Data are expressed as mean ± standard error of mean using an unpaired t test. P ≤ 0.05 was considered statistically significant (∗P ≤ 0.05; ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.001). The N values refer to the number of individual animals.