Duoset human trem 1

Manufactured by R&D Systems

Sourced in United States

The DuoSet-Human TREM-1 is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) developed for the measurement of human TREM-1 in cell culture supernatants, serum, and plasma. TREM-1 is a cell surface receptor that is upregulated during inflammation and infection.

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DuoSets (49 citations) Human TREM-1 DuoSet ELISA kit (2 citations)

5 protocols using duoset human trem 1

Metabolic and Inflammatory Biomarkers

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We performed blood collection by venipuncture after 12 hours of fasting and without alcohol, coffee, or tea intake for 24 hours. The heparin and plasma samples were separated by centrifugation at 2465 g at 5°C for 15 minutes and stored at -80°C. Serum concentrations (mg/dL) of glucose, total cholesterol, high-density lipoprotein (HDL-c), and triglycerides were analyzed by colorimetric or turbidimetric method. The serum concentration (IU/mL) of insulin was analyzed by electrochemiluminescence. HOMA-IR value was calculated from the fasting insulin and glucose dosages. Plasma concentrations of Ox-LDL and serum levels of the inflammatory molecule sTREM-1 were analyzed by enzyme-linked immunosorbent assay (ELISA), using the specific analysis kit (Mercodia, Uppsala, Sweden) and DuoSet-Human TREM-1 (R&D Systems, Minneapolis, USA).

Vázquez C.M., Costa J.O., Bomfim L.G., Pires L.V., da Silva D.G., Fukutani K.F., de Jesus A.R., de Jesus Silva N., de Jesus Santana G., de Moura T.R, & Barbosa K. (2019). Oxidized Low-Density Lipoprotein (Ox-LDL) and Triggering Receptor-Expressed Myeloid Cell (TREM-1) Levels Are Associated with Cardiometabolic Risk in Nonobese, Clinically Healthy, and Young Adults. Oxidative Medicine and Cellular Longevity, 2019, 7306867.

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Measuring Systemic sTREM-1 and MMP-8 Levels

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Systemic levels of sTREM-1 and matrix metalloproteinases (MMP)-8 were measured in plasma using an ELISA kit (DuoSet-Human TREM-1 and DuoSet-Human Total MMP-8, R&D System, Minneapolis, MN, USA) according to the manufacturer’s specifications.

da Silva-Neto P.V., de Carvalho J.C., Pimentel V.E., Pérez M.M., Toro D.M., Fraga-Silva T.F., Fuzo C.A., Oliveira C.N., Rodrigues L.C., Argolo J.G., Carmona-Garcia I., Neto N.T., Souza C.O., Fernandes T.M., Bastos V.A., Degiovani A.M., Constant L.F., Ostini F.M., Feitosa M.R., Parra R.S., Vilar F.C., Gaspar G.G., da Rocha J.J., Feres O., Frantz F.G., Gerlach R.F., Maruyama S.R., Russo E.M., Viana A.L., Fernandes A.P., Santos I.K., Bonato V.L., Boechat A.L., Malheiro A., Sadikot R.T., Dias-Baruffi M., Cardoso C.R., Faccioli L.H, & Sorgi C.A. (2021). sTREM-1 Predicts Disease Severity and Mortality in COVID-19 Patients: Involvement of Peripheral Blood Leukocytes and MMP-8 Activity. Viruses, 13(12), 2521.

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Quantitative TREM-1, MMP-2, and MMP-8 Assay

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sTREM-1, MMP-2, and MMP-8 levels were measured in TAF samples using an ELISA kit (DuoSet-Human TREM-1, DuoSet-Human Total MMP-2, and DuoSet-Human Total MMP-8, R&D System, Minneapolis, MN, USA) according to the manufacturer’s specifications.

da Silva-Neto P.V., do Valle V.B., Fuzo C.A., Fernandes T.M., Toro D.M., Fraga-Silva T.F., Basile P.A., de Carvalho J.C., Pimentel V.E., Pérez M.M., Oliveira C.N., Rodrigues L.C., Bastos V.A., Tella S.O., Martins R.B., Degiovani A.M., Ostini F.M., Feitosa M.R., Parra R.S., Vilar F.C., Gaspar G.G., da Rocha J.J., Feres O., Arruda E., Maruyama S.R., Russo E.M., Viana A.L., Santos I.K., Bonato V.L., Cardoso C.R., Tanus-Santos J.E., Donadi E.A., Faccioli L.H., Dias-Baruffi M., Fernandes A.P., Gerlach R.F, & Sorgi C.A. (2022). Matrix Metalloproteinases on Severe COVID-19 Lung Disease Pathogenesis: Cooperative Actions of MMP-8/MMP-2 Axis on Immune Response through HLA-G Shedding and Oxidative Stress. Biomolecules, 12(5), 604.

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Quantification of Soluble TREM-1 and Inflammatory Mediators

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Soluble (s)TREM-1 was quantified in cell-free Mf supernatants by human TREM-1 DuoSet (R&D Systems). Secreted IL-12, TNFα, IL-1β, CXCL8, IL-6, and IL-10 (Peprotech, Milano), osteopontin (OPN), CCL18, CCL24 and TGFβ1 (R&D Systems, Space Import Export, Milano, Italy) content in Mf CM was also measured by specific ELISA. HMGB1 levels were measured in SF and plasma from OJIA patients and age-matched controls using HMGB1 ELISA kit II (IBL International, Hamburg, Germany); the lower limit of detection was 0.3 ng/mL. Optical density was determined using a Spectrafluor Plus plate reader from TECAN (Milano, Italy). All assays were done in duplicate.

Raggi F., Pelassa S., Pierobon D., Penco F., Gattorno M., Novelli F., Eva A., Varesio L., Giovarelli M, & Bosco M.C. (2017). Regulation of Human Macrophage M1–M2 Polarization Balance by Hypoxia and the Triggering Receptor Expressed on Myeloid Cells-1. Frontiers in Immunology, 8, 1097.

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Human TREM-1 DuoSet ELISA Protocol

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Human TREM-1 DuoSet (R&D Systems Europe, Abingdon, UK) was performed according to manufacturer’s instruction. When indicated buffer solutions where replaced by buffers (coating and blocking buffer = pcb = protein containing buffer) described above at Radsak ELISA.

Hasibeder A., Stein P., Brandwijk R., Schild H, & Radsak M.P. (2015). Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay. Scientific Reports, 5, 15381.

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