To characterize the phenotype of memory, effector, and naïve cells, 106 peritoneum cells were stained with the following antibodies: hamster anti-mouse CD3ϵ FITC (isotype hamster IgG1, κ), rat anti-human/mouse CD44 PE (isotype rat IgG2b, κ), rat anti-mouse CD4 Percp-Cy5.5 (isotype rat IgG2a, κ, BD Biosciences), or rat anti-mouse CD62L APC (isotype rat IgG2a, κ).
Hamster anti mouse cd3 fitc
Manufactured by BD
Hamster anti-mouse CD3ϵ FITC is a fluorescently labeled antibody that binds to the CD3ϵ (epsilon) subunit of the T cell receptor complex in mice. It is a tool used for the identification and enumeration of T cells in flow cytometry applications.
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2 protocols using hamster anti mouse cd3 fitc
1
Phenotypic Characterization of Memory and Naive Cells
2
Multiparameter Flow Cytometry of Peritoneal Cells
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For extracellular staining, 106 peritoneum cells were stained with the following antibodies: hamster anti-mouse CD3ϵ FITC (isotype hamster IgG1, κ), rat anti-mouse CD49b PE (isotype rat IgM, κ, BD Biosciences), rat anti-mouse CD19 Percp-Cy5.5 (isotype rat IgG2a, κ, BD Biosciences), and rat anti-mouse CD138 APC (isotype rat IgG2a, κ, BD Biosciences). In parallel, 106 peritoneum cells were used to determine the monocyte phenotype, using rat anti-mouse CD11b PE (isotype rat IgG2b, κ, BD Biosciences) and rat anti-mouse F4/80 APC (isotype rat IgG2a, κ) antibodies.
Data were read in a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ) and analyzed with the software CellQuest (Becton Dickinson).
Data were read in a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ) and analyzed with the software CellQuest (Becton Dickinson).
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