Dnase 1 conjugated with alexa fluor 594

Mouse monoclonal anti-β- and anti-γ-actin antibodies directed against epitopes present on the N-terminus of β- or γ-actin were purchased from Sigma-Aldrich (clone AC-15 or clone 2–2.1.14.17, respectively) or from AbD Serotec (clone 4C2 or clone 2A3 respectively). Mouse monoclonal anti-GFP antibodies were obtained from Santa Cruz Biotechnology. Phalloidin conjugated with Alexa Fluor 568 and DNase I conjugated with Alexa Fluor 594 (detecting unpolymerized actin) were purchased from Invitrogen. Rabbit anti-myosin II antibodies as well as mouse monoclonal antibodies against ezrin were purchased from Sigma-Aldrich. Secondary anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 568 were obtained from Invitrogen, while anti-mouse HRP-linked antibodies were from Cell Signaling. Fetal bovine serum (FBS), glutamine, penicillin/streptomycin, trypsin, alpha-MEM media and Lipofectamine 2000 were obtained from Invitrogen. DNase I from bovine pancreas and DNA from calf thymus were purchased from Sigma-Aldrich. Collagen type I was purchased from Corning, while epidermal growth factor (EGF) was obtained from BD Biosciences. Dako fluorescent mounting medium was obtained from Dako. All other reagents were classified as analytical grade reagents.

Mouse, monoclonal anti-β (clone AC-15) and anti-γ (clone 2–2.1.14.17) actin antibodies corresponding to epitopes located in the N-terminal end of β- or γ-actin, respectively, and mouse, monoclonal anti-β-tubulin antibodies were purchased from Sigma-Aldrich. Mouse, monoclonal anti-GFP antibody and sodium butyrate were obtained from Santa Cruz Biotechnology. DNase I conjugated with Alexa Fluor^® 594 used to detect unpolymerized actin and Alexa Fluor^® 568-conjugated phalloidin were obtained from Invitrogen. Secondary anti-mouse antibodies conjugated with DyLight^® 549 were purchased from Jackson ImmunoResearch, and anti-mouse HRP-linked antibodies were from Cell Signaling. Fetal bovine serum (FBS), trypsin, glutamine, penicillin/streptomycin, G-418 (Geneticin), DMEM and alpha-MEM media and Lipofectamine™ 2000 were purchased from Invitrogen. DNA from calf thymus and DNase I from bovine pancreas were from Sigma-Aldrich. Dako cytomatic fluorescent mounting medium was obtained from Dako. Matrigel™ and Epidermal Growth Factor (EGF) were obtained from BD Biosciences. All other chemicals were classified as analytical grade reagents.