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2 protocols using importin4

1

Chromatin Assembly Factor-1 Regulation

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ASF1a/b (11 (link)), CAF-1/p150 (Novus Biologicals #NB500–207A1), DAXX (Santa Cruz #sc-7152), HAT1 (Abcam #ab12164), HIRA (Abcam #ab20655), Histone H3 (Abcam #ab7834), H3K9me1 (Millipore #07–450), H3K9me2 (Millipore, #07–212), H4K12ac (Millipore #07–595), Hsc70 (Abcam #ab19136), Hsp90 (Santa Cruz #sc-7947), Importin4 (Abcam #ab28387), MCM2 (BD Transduction Lab #610700), MCM5 (Bethyl A300–195A), NASP (donated by Dr Almouzni), RPL5 (Abcam #ab74744), RPS3a (Abcam, ab171742), SetDB1 (Abcam #ab12317). For Western blot analysis the primary antibodies were detected with a horseradish peroxidase-conjugated secondary antibody, developed with enhanced chemiluminescence (ECL, Pierce) and exposed onto an X-ray film.
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2

Chromatin Remodeling Protein Characterization

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Asf1 (Dr. Almouzni), β-actin (Sigma Aldrich A5316), Flag (Sigma Aldrich F3165), γH2AX (Abcam ab2893), HA (Sigma clone 12CA5), HAT1 (Abcam ab12164), HIRA (Abcam ab20655), Histone H3 (Abcam ab7834), H3ac (Merck Millipore, 06-599), Histone H4 (Abcam ab10158), H4K12ac (Merck Millipore 07-595), Hsc70 (Abcam ab19136), Hsp90 (Santa Cruz sc-7947), Importin4 (Abcam ab283887), JMJD1B (Cell Signaling Technology 2621S), NASP (Dr. Almouzni), Topo I (Santa Cruz sc-32736), SetDB1 (Abcam ab12317).
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