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Oxalacetate

Manufactured by Merck Group
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Oxalacetate is a key organic compound used in various laboratory applications. It is an important intermediate in the citric acid cycle, a central metabolic pathway found in most living organisms. Oxalacetate serves as a substrate for multiple enzymatic reactions, making it a valuable tool for researchers studying biochemical processes.

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Lab products found in correlation

Pyridoxal phosphate, by Merck Group (1 mentions) Ribose, by Merck Group (1 mentions) Dihydroxyacetone phosphate, by Merck Group (1 mentions) Pyruvate, by Merck Group (1 mentions) Xylose, by Merck Group (1 mentions) Mannose, by Merck Group (1 mentions) Arabinose, by Merck Group (1 mentions) Galactose, by Merck Group (1 mentions) 2 oxoglutarate, by Merck Group (1 mentions) Erythrose 4 phosphate, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Fructose, by Merck Group (1 mentions) Acetyl coa, by Merck Group (1 mentions) Tris buffer, by Bio-Rad (1 mentions) Xmark microplate spectrophotometer, by Bio-Rad (1 mentions)

2 protocols using oxalacetate

1

Comprehensive Metabolomics Standards Protocol

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All metabolite standards: arabinose, galactose, glucose, mannose, ribose, erythrose-4-phosphate, pyridoxal phosphate, xylose, acetone, fructose, pyruvate, 2-oxobutanoate, 2-oxoglutarate, oxalacetate, dihydroxyacetone phosphate, as well as glacial acetic acid (AcOH) and methanol (MeOH) (Optima grade) were purchased from Sigma Aldrich (St. Louis, MO). 18 MΩ water was obtained using the ultrapure water system (Barnstead, Dubuque, IA).
Lane A.N., Arumugam S., Lorkiewicz P.K., Higashi R.M., Laulhé S., Nantz M.H., Moseley H.N, & Fan T.W. (2015). Chemoselective detection and discrimination of carbonyl-containing compounds in metabolite mixtures by 1H-detected 15N NMR. Magnetic resonance in chemistry : MRC, 53(5), 337-343.
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2

Citrate Synthase Activity Measurement

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Citrate Synthase (CS) activity in red and white gastrocnemius muscle was assayed as previously described [37 ]. Briefly, after the addition of 5 µL of muscle homogenate (0.175 mM KCl & 2 mM EDTA, pH 7.4), 230 µL of reagent cocktail ((3 mM Acetyl CoA (Sigma A-2056), 100 mM Tris buffer (BioRad, Hercules, CA, USA); pH 8.3), 1 mM DTNB (Sigma D-8130) and 15 µL of 10 mM Oxalacetate (Sigma 0-4126). CS activity was measured at 412 nm (xMark™ Microplate Spectrophotometer, BioRad). Enzyme activity was expressed relative to wet weight, change in absorbance/min was calculated to measure CS activity [38 (link)].
O’Keefe L., Vu T., Simcocks A.C., Jenkin K.A., Mathai M.L., Hryciw D.H., Hutchinson D.S, & McAinch A.J. (2022). CB1 Ligand AM251 Induces Weight Loss and Fat Reduction in Addition to Increased Systemic Inflammation in Diet-Induced Obesity. International Journal of Molecular Sciences, 23(19), 11447.
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