Roswell Park Memorial Institute 1640 (RPMI 1640) and fetal bovine serum (FBS) were purchased from Vitrocell (Sao Paulo, Brazil). Ketamine and xylazine were obtained from Ceva Santé Animale (Paulínea, Brazil). Oyster glycogen, propidium iodide (PI), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), trypan blue, CoCl2 and Ringer–Locke solution were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Anti-Ly6G antibody conjugated with phycoerythrin (PE) and annexin V (AnxV)–fluorescein isothiocyanate (FITC) were purchased from BD Biosciences (San Jose, CA, USA). Percoll was obtained from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). Enzyme-linked immunosorbent assay (ELISA) kits were obtained as follows: TGF-β and IL-10 were obtained from R&D Systems, Inc. (Minneapolis, MN, USA); VEGF-A was obtained from IBL (Mikasa-Shi, Japan) and ELISA, arginase-1 and MMP-9 were obtained from MyBioSource (San Diego, CA, USA). Transwell insert plate was purchased from Costar (Cambridge, MA, USA).
Mmp 9
Manufactured by MyBioSource
Sourced in United States
MMP-9 is a laboratory enzyme product. It functions as a matrix metalloproteinase, which is an enzyme involved in the breakdown of extracellular matrix proteins.
Automatically generated - may contain errors
Lab products found in correlation
Mmp 2, by MyBioSource (2 mentions)
Oyster glycogen, by Merck Group (1 mentions)
Transwell insert plate, by Corning (1 mentions)
Cocl2, by Merck Group (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Il 10, by R&D Systems (1 mentions)
Percoll, by Cytiva (1 mentions)
Ketamine, by Ceva (1 mentions)
Xylazine, by Ceva (1 mentions)
Fetal bovine serum (fbs), by Vitrocell (1 mentions)
Hyaluronic acid, by R&D Systems (1 mentions)
Histamine, by MyBioSource (1 mentions)
Interleukin 6 (il 6), by Proteintech (1 mentions)
Mmp 2, by R&D Systems (1 mentions)
Mmp 1, by MyBioSource (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Mmp 9, by R&D Systems (1 mentions)
Tgf β, by MyBioSource (1 mentions)
Interleukin 6 (il 6), by MyBioSource (1 mentions)
Tnf α, by MyBioSource (1 mentions)
3 protocols using mmp 9
1
Murine Neutrophil Isolation and Characterization
2
Quantification of Biochemical Markers
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We extracted blood samples from the heart of the test mice on the final day of experiments. The plasma levels of MMP-1, MMP-2, MMP-9, histamine, hyaluronidase, hyaluronic acid, IL-6, TNF-α, and AGEs were determined using commercial ELISA kits (MMP-1: MyBioSource, San Diego, CA, USA; MMP-2, MMP-9, hyaluronic acid, and TNF-α: R&D Systems, Minneapolis, MN, USA; histamine: Bertin Pharm., Montigny-le-Bretonneux, France; hyaluronidase: AB Clonal Inc., Tokyo, Japan; IL-6: Proteintech, Rosemont, IL, USA; and AGEs: OxiSelect AGE connection ELISA kit, Cell Biolabs Inc., San Diego, CA, USA), according to the instructions of the respective manufacturers.
3
Extracellular Protein Expression Analysis
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Supernatants obtained from cell cultures treated with IC50 conditions of the respective NPs were treated according to the manufacturer’s protocols for the assessment of the expression of different extracellular proteins and soluble mediators by ELISA. The following mouse kits from MyBiosourse, USA, were utilized: collagen1 (MBS724458), TGF-β (MBS160136), MMP2 (MBS824667), MMP9 (MBS175917), IL-6 (MBS824703), and TNF-α (MBS825075).
Mouse alpha-smooth muscle actin (α-SMA) ELISA kit (MBS267551, MyBioSource, USA) was also used to determine the expression level of the intracellular α-SMA in Fbls and TAFs according to the manufacturer’s protocol.
Mouse alpha-smooth muscle actin (α-SMA) ELISA kit (MBS267551, MyBioSource, USA) was also used to determine the expression level of the intracellular α-SMA in Fbls and TAFs according to the manufacturer’s protocol.
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