Multicycle for windows version 3

Confluent cells were seeded in an appropriate density followed by addition of sodium selenite 24 h after cell seeding. The irradiation with single-doses of 8 Gy or 0 Gy (control) as duplicates for each experimental approach was performed 24 h after sodium selenite addition and carried out in three independent experiments. 24 h or 48 h after irradiation cells were fixed and permeabilized 10 min in ethanol (70% (v/v), − 20 °C), and stained with propidium iodide (75 µM). Samples were measured on flow cytometer Cytomics FC 500 (Beckman Coulter, Krefeld, Germany). Analysis was performed using Multicycle for Windows, version 3.0 (Phoenix Flow Systems, San Diego, USA).

OUMS-27 cells were grown in each well with various concentrations of CGP or DMSO for 24 h. Harvested cells were fixed in cold 70% (v/v) ethanol. CycleTEST™ PLUS DNA reagent kit (BD Biosciences) was used according to the manufacturer’s protocol. The cell cycle distribution in all sample cells was measured by flow cytometry (EPICS Elite ESP; Beckman Coulter Co., Brea, CA, USA) and the percentage of cells in each phase of the cell cycle was analyzed using a Multi Cycle for Windows Version 3.0 (PHOENIX, San Diego, CA, USA).

Cells were seeded in an appropriate density followed by medium exchange with serum free medium 24 h after cell seeding to induce synchronisation of cell cycle. Further 24 h later, SVA was added in different concentrations (0.05 μM, 0.1 μM, 0.5 μM, 1 μM). The irradiation with single-doses of 2 Gy or 0 Gy (control) for each experimental approach was performed 24 h after SVA addition and carried out at least in three independent experiments. 24 h or 72 h after irradiation cells were fixed and permeabilized 10 min in ethanol (70% (v/v), −20 °C), and stained with propidium iodide (75 μM). Samples were measured on flow cytometer Cytomics FC 500 (Beckman Coulter, Krefeld, Germany). Analysis was performed using Multicycle for Windows, version 3.0 (Phoenix Flow Systems, San Diego, USA).