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Sep pak tc18 μelution plate

Manufactured by Waters Corporation
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The Sep-Pak® tC18 μElution Plate is a solid-phase extraction (SPE) plate designed for sample preparation in analytical workflows. It utilizes a C18 sorbent material to selectively retain and concentrate analytes from complex matrices prior to instrumental analysis.

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Lab products found in correlation

Vacuum manifold, by Merck Group (1 mentions) 5600 tripletof analyzer qqtof, by AB Sciex (1 mentions)

Close spelling variants of this product

Sep-Pak (142 citations) TC18 Sep-Pak (33 citations)

2 protocols using sep pak tc18 μelution plate

1

iTRAQ-Labelled Peptide 2D-LC-MS/MS

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The 2D LC separation of our iTRAQ-labelled samples was carried out as reported previously [23 ]. Ninety-six μg (via multiple injections) of the labelled peptide mixture was injected using the micro-pickup mode into a Zorbax Bio-SCX II column (Agilent; SantaClara, CA, USA). A total of 78 fractions were eluted (2 min each) and collected on a 96 well v-bottom plate. The eluted fractions were subsequently combined to 11 fractions and then desalted with Sep-Pak® tC18 μElution Plate (WATERS) using a vacuum manifold (Millipore; Billerica, MA, USA) in accordance to the manufacturer's recommendations before second-dimension reversed-phase (RP) chromatography. The MS analysis was performed using a 5600 TripleTOF analyzer (QqTOF; AB SCIEX) in Information Dependent Mode.
Narasimhan K., Lee Y.M., Lim T.K., Port S.A., Han J.H., Chen C.S, & Lin Q. (2015). Genistein exerts anti-leukemic effects on genetically different acute myeloid leukemia cell lines by inhibiting protein synthesis and cell proliferation while inducing apoptosis – molecular insights from an iTRAQ™ quantitative proteomics study. Oncoscience, 2(2), 111-124.
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2

Desalting and Reconstitution of Samples

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Digested samples were desalted using a Sep-Pak tC18 μElution Plate (Waters) following the manufacturer's instructions. After elution in 60% acetonitrile, 0.1% TFA, samples were dried down and reconstituted in 50 μl 0.1% formic acid, 0.05% TFA. Samples were briefly sonicated, vortexed, centrifuged, and transferred to autosampler vials for LC-MS/MS analysis.
Zappacosta F., Wagner C.D., Della Pietra A I.I.I., Gerhart S.V., Keenan K., Korenchuck S., Quinn C.J., Barbash O., McCabe M.T, & Annan R.S. (2021). A Chemical Acetylation-Based Mass Spectrometry Platform for Histone Methylation Profiling. Molecular & Cellular Proteomics : MCP, 20, 100067.
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