Sigma laminin

Manufactured by Merck Group

Sigma)/laminin is a laboratory product manufactured by Merck Group. It is a component used in cell culture and cell biology research applications. The core function of Sigma)/laminin is to provide a substrate for cell attachment and growth in in vitro studies.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (1 mentions) Psa ncam microbeads, by Miltenyi Biotec (1 mentions) Sonic hedgehog, by R&D Systems (1 mentions) Poly d lysine, by Merck Group (1 mentions) Lab tek chamber, by Thermo Fisher Scientific (1 mentions) 48 well plate, by Greiner (1 mentions)

Spelling variants of this product

Laminin (1233 citations) Laminin (Sigma L9393, (2 citations) APoly-D-lysine-laminin (Sigma)-coated 12 well-plate (2 citations)

3 protocols using sigma laminin

Differentiation of Fragile-X iPSCs to Neurons

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Fibroblasts from a clinically healthy male control (GM08330) or a diagnosed Fragile‐X Syndrome male patient (GM05848) were purchased from Coriell Institute for Medical Research and used to derive induced pluripotent stem cell (iPSC) clones and subsequent stable, homogeneous neural progenitor cells (NPCs) as described 88. NPCs were expanded in 70% DMEM (Invitrogen), 30% Ham's F‐12 (Mediatech), supplemented with B‐27 (Invitrogen), 20 ng/ml EGF (Sigma), and 20 ng/ml bFGF (R&D Systems) on poly‐ornithine (Sigma)/laminin (Sigma)‐coated culture plates. Neural differentiation was induced by growth factor removal in the same media for 15 days before harvest. Cells were harvested by scraping and pelleting followed by total RNA (including miRNAs) isolation using a miRNeasy Mini Kit (Qiagen). Biological triplicates were collected from each undifferentiated NPC and differentiated neuron cultures from control 8330‐8 and two clones from the FXS patient: 848‐1 and 848‐3 88.

Ramaiah M., Tan K., Plank T.M., Song H., Dumdie J.N., Jones S., Shum E.Y., Sheridan S.D., Peterson K.J., Gromoll J., Haggarty S.J., Cook‐Andersen H, & Wilkinson M.F. (2018). A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1. EMBO Reports, 20(2), e46566.

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Motor Neuron Differentiation of Rat iPSCs

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Both rat iPSCs, i.e., o-riPSCs and l-riPSCs, were expanded on feeder cell layers in basic-DMEM. For the expansion of rat iPSCs, 1000 U/mL LIF was added in basic-DMEM. For neurogenesis, riPSCs were incubated for 2 days without LIF and compacted to form embryoid bodies (EBs) in hanging drops. After that, EBs were exposed to 5×10^-5 M all-trans retinoic acid (RA, Sigma) in basic DMEM for 5 days and were placed in a magnetic cell separation system (MACS; Miltenyi Biotech; Auburn, CA; www.miltenyibiotec.com) by using PSA-NCAM micro beads (130-092-966 Miltenyi Biotech). The sample preparation, magnetic labeling, and magnetic separation with LS columns were conducted according to the manufacturer's instructions. Sorted neuron progenitors were attached to dishes coated with poly-d-lysine hydrobromide (P7280, Sigma)-laminin (L2020, Sigma), and then they were differentiated into motor neurons by addition of 25 ng/mL sonic hedgehog (recombinant mouse sonic hedgehog N-terminal 461-SH; R&D Systems; Minneapolis, MN; www.RnDSystems.com) and 2×10^-5 M RA in basic-DMEM. The culture medium was changed every day.

Takenaka-Ninagawa N., Kawabata Y., Watanabe S., Nagata K, & Torihashi S. (2014). Generation of Rat-Induced Pluripotent Stem Cells from a New Model of Metabolic Syndrome. PLoS ONE, 9(8), e104462.

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Neural Stem Cell Transduction with AAV

Cited 1 time

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For RNA-seq, NSCs were seeded in 48-well plates (Greiner Bio-One) and incubated overnight. AAV library #1 or library #3 (same libraries as in Weinmann et al.;⁵³ (link) multiplicity of infection [MOI]: 10,000) was added to the media and remained for the duration of 7 days. For IHC, Labtek chambers (Thermo Fisher Scientific) were coated with Poly D-Lysine (PDL; Sigma)/laminin (Sigma), and NSCs were seeded at a density of 2 × 10⁴ cells per square centimeter overnight. AAVs were added (MOI: 10,000) and remained in the media for 1, 3, 5, or 7 days.

Kremer L.P., Cerrizuela S., Dehler S., Stiehl T., Weinmann J., Abendroth H., Kleber S., Laure A., El Andari J., Anders S., Marciniak-Czochra A., Grimm D, & Martin-Villalba A. (2021). High throughput screening of novel AAV capsids identifies variants for transduction of adult NSCs within the subventricular zone. Molecular Therapy. Methods & Clinical Development, 23, 33-50.

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