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Embelin

Manufactured by Abcam
Sourced in United Kingdom

Embelin is a natural compound isolated from the fruit of the Embelia plant. It is commonly used as a research tool in laboratory settings. Embelin has reported biological activities, but its specific core function is not provided here to maintain an unbiased and factual approach.

Automatically generated - may contain errors

2 protocols using embelin

1

Osteosarcoma Cell Line Cytotoxicity Assay

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The osteosarcoma MG-63 (code no: TCHu 124) and U2OS (code no: TCHu 88) cell lines were purchased from the Cell Research Center of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 medium, Dulbecco's modified Eagle's high glucose medium (DMEM) and trypsin were purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Embelin was obtained from Abcam (Cambridge, UK). Pan-caspase inhibitor z-VAD-fmk and caspase-8 inhibitor z-IETD-fmk were purchased from Abcam (Cambridge, UK). Rabbit antibodies against caspases 3 and 8, death receptor (DR) 5, XIAP and MMP-9 were purchased from Abcam.
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2

Embelin Induces Apoptosis in Osteosarcoma

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The osteosarcoma U-2 OS and MG63 cell lines were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Roswell Park Memorial Institute-1640 (RPMI-1640) medium, Dulbecco's Modified Eagle Medium (DMEM) high glucose medium, and trypsin were bought from Gibco (Gaithersburg, MD, USA). Embelin was purchased from Abcam (Cambridge, UK). 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO, USA). Rabbit antibody of caspases (3, 8, and 9), PI3K, Akt, p-Akt, XIAP, and MMP-9 were bought from Abcam (Cambridge, UK). Cleaved caspase-3 was purchased from Cell Signaling Technology (Boston, USA).
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