Embelin

Manufactured by Abcam

Sourced in United Kingdom

Embelin is a natural compound isolated from the fruit of the Embelia plant. It is commonly used as a research tool in laboratory settings. Embelin has reported biological activities, but its specific core function is not provided here to maintain an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (2 mentions) Mmp 9, by Abcam (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Z ietd fmk, by Abcam (1 mentions) High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Z vad fmk, by Abcam (1 mentions) Dulbecco s modified eagle medium dmem high glucose medium, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) P akt, by Abcam (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions)

2 protocols using embelin

Osteosarcoma Cell Line Cytotoxicity Assay

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The osteosarcoma MG-63 (code no: TCHu 124) and U2OS (code no: TCHu 88) cell lines were purchased from the Cell Research Center of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 medium, Dulbecco's modified Eagle's high glucose medium (DMEM) and trypsin were purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Embelin was obtained from Abcam (Cambridge, UK). Pan-caspase inhibitor z-VAD-fmk and caspase-8 inhibitor z-IETD-fmk were purchased from Abcam (Cambridge, UK). Rabbit antibodies against caspases 3 and 8, death receptor (DR) 5, XIAP and MMP-9 were purchased from Abcam.

Qian H., Chen Y., Huang T., Liu T., Li X., Jiang G., Zhang W., Cheng S, & Li P. (2018). Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis. Oncology Letters, 15(5), 6931-6940.

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Embelin Induces Apoptosis in Osteosarcoma

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The osteosarcoma U-2 OS and MG63 cell lines were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Roswell Park Memorial Institute-1640 (RPMI-1640) medium, Dulbecco's Modified Eagle Medium (DMEM) high glucose medium, and trypsin were bought from Gibco (Gaithersburg, MD, USA). Embelin was purchased from Abcam (Cambridge, UK). 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO, USA). Rabbit antibody of caspases (3, 8, and 9), PI3K, Akt, p-Akt, XIAP, and MMP-9 were bought from Abcam (Cambridge, UK). Cleaved caspase-3 was purchased from Cell Signaling Technology (Boston, USA).

Qian H., Huang T., Chen Y., Li X., Gong W., Jiang G., Zhang W., Cheng S., Li X, & Li P. (2018). X-linked inhibitor of apoptosis protein inhibitor Embelin induces apoptosis via PI3K/Akt pathway and inhibits invasion in osteosarcoma cells. Journal of cancer research and therapeutics, 14(Supplement).

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