Balb/c-nu mice aged 4 weeks were purchased from the Animal Institute of the Chinese Academy of Medical Science. All animal experiments were performed in accordance with the institutional guidelines of the Hunan Normal University. The University Committee on Animal Care and Hunan Normal University approved the experimental protocols (No. 2015-146). Mice were randomly divided into 3 groups (4 mice/group) and maintained under standard conditions, according to standard protocols. Cells were suspended in serum-free DMEM/Matrigel (BD Biosciences) mixture (1:1 volume). Each recipient Balb/c-nu mouse was inoculated subcutaneously with H460-derived LCSLCs (1×103, 1×104, and 1×105 cells) in 1 flank and monolayer H460 cells (1×104, 1×105, and 1×106) in the other, respectively. Tumorigenicity experiments were terminated 1 month after cell inoculation. The harvested tumors were imaged and weighed immediately. After that, specimens from tumor tissue samples were fixed with 10% neutral-buffered formalin, processed in paraffin blocks, and sectioned. The sections were stained with hematoxylin and eosin (H&E) and examined for histopathology.
Dmem matrigel
Manufactured by BD
Sourced in United States
DMEM/Matrigel is a cell culture medium formulation that combines Dulbecco's Modified Eagle's Medium (DMEM) and Matrigel, a basement membrane extract. DMEM provides a nutrient-rich environment to support cell growth and proliferation, while Matrigel offers a three-dimensional extracellular matrix to facilitate cell attachment and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Transwell chamber, by Corning (3 mentions)
Nod scid mice, by BD (2 mentions)
Ab5603, by Merck Group (1 mentions)
Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Ultravision detection system, by Lab Vision (1 mentions)
3 ma m9281, by Merck Group (1 mentions)
C57bl 6, by BD (1 mentions)
Nod cg prkdcscidil2rgtmlwjl szj, by Jackson ImmunoResearch (1 mentions)
Szx16, by Olympus (1 mentions)
Nod cb17 prkdcscid j nod scid mice, by Charles River Laboratories (1 mentions)
Cell counting kit 8 (cck8), by Beyotime (1 mentions)
Matrigel, by BD (1 mentions)
D 35578, by Leica (1 mentions)
Transwell chamber, by Merck Group (1 mentions)
26 protocols using dmem matrigel
1
Tumorigenicity of Lung Cancer Stem-Like Cells
2
Melanoma Spheres: BrdU, Apoptosis, and SOX2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BrdU pulses in melanoma spheres were performed for 8 h (4 μg/ml). Spheres were plated on DMEM/Matrigel (1:40) (BD Biosciences) to allow attachment, fixed with 4% paraformaldehyde and processed as previously described.60 (link) For apoptosis, spheres were immunolabeled with a rabbit anti-cleaved Caspase3 (Cell Signaling Technologies, Danvers, MA, USA), followed by an anti-rabbit fluorescein isothiocyanate-conjugated secondary antibody. For SOX2 immunofluorescence, spheres were immunolabeled with a rabbit anti-SOX2 antibody (AB5603; Millipore, Billerica, MA, USA) and with an anti-rabbit fluorescein isothiocyanate-conjugated secondary antibody. Formalin-fixed paraffin-embedded sections were subjected to antigen retrieval (with citrate buffer pH 6.0), incubated with rabbit anti-SOX2 antibody (AB5603; Millipore) and rabbit anti-cleaved Caspase3 (Cell Signaling Technologies) and visualized using UltraVision Detection System (Lab Vision, Fremont, CA, USA) and diaminobenzidine (Dako, Carpinteria, CA, USA).
3
Xenograft Model of Huh7 Cells in NOD/SCID Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NOD.CB17-Prkdcscid/J (NOD/SCID) male mice were purchased from Charles River Laboratories, Inc. (Wilmington, MA, USA). Mice were housed under specific pathogen-free conditions with a 12 h light/dark cycle and provided ad libitum access to tap water and food. Huh7 cells (approximately 1.0 × 106 cells) were resuspended in 200 μL of a 1:1 DMEM:Matrigel (BD Biosciences) mixture with control IgG (n = 3, 100 mg/106 cells) or anti-DKK-1 neutralizing antibody (n = 3, 100 mg/106 cells) and subcutaneously injected into 6-week-old NOD/SCID mice. Mice were euthanized, and the tumor volume was evaluated on day 28 after xenotransplantation. The experimental protocol was approved by the Kanazawa University Animal Care and Use Committee and conformed to the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences.
4
Xenograft Tumor Establishment and Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male BALB/c nude mice at 4 weeks of age were purchased from the Animal Center of Xi'an Jiaotong University and housed in a specific pathogen‐free facility with free access to autoclaved food and water. To establish xenograft tumors, individual mice were randomized and implanted s.c. with 1 × 106 A549‐NC, A549‐LKB1, A549‐LKB1S, HCC827‐NC, HCC827‐LKB1, or HCC827‐LKB1S cells (n = 6) in 100 μL DMEM/Matrigel (No. 356234; BD, Franklin Lakes, NJ, USA) into their back. In addition, another set of mice were randomized and implanted s.c. with 1 × 106 A549‐NC, A549‐LKB1S, HCC827‐NC, or HCC827‐LKB1S cells. When a visible tumor was established, the mice bearing A549‐LKB1S and HCC827‐LKB1S cells were randomized and injected intratumorally with 100 μL PBS as the A549‐LKB1S and HCC827‐LKB1S group or with 5 mmol/L 3‐MA (M9281; Sigma) every other day for 2 weeks. The growth of implanted tumors was monitored every other day for their volumes that were calculated using the formula: V = 0.5 × length × width2.
5
Establishing Murine Subcutaneous Tumor Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B16 EV, B16 Q152H, B16 PCSK9, and B16 D374Y cells (1 × 105/per mice) were counted and suspended in 0.1 ml DMEM/Matrigel (BD) mixture with 1:1 volume and implanted subcutaneously into the left flank of 8-week-old C57BL/6 (The Jackson Laboratory) or Pcsk9−/− (Dr. Seidah) male mice. After implantation of cancer cells, the mice were monitored through observation and palpation. The size of the tumors was measured every two days by caliper. Tumor volume was calculated based on the formula V = L × W2 × 0.52. Animals were euthanized when the tumor volume reached endpoint (tumor size > 1000 mm3 or poor body conditions). The allograft tumors were collected and processed for subsequent analysis.
6
Xenograft Tumor Transplantation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Disaggregated cells and organoids were resuspended in 100-200 μl of a 1:1 v/v mixture of cold DMEM:Matrigel (BD Biosciences, San Jose, CA) and kept on ice until transplantation. For transplants of intact cell fragments, a small incision was made in the skin in the flank area, and then fragments were inserted using forceps. Xenograft-bearing mice were euthanized when the size of the tumors approached 1 ml in volume (adding together the sizes of individual growths when more than one was present). Serially transplanted aliquots represented ~0.1%-0.3% of the xenograft tumor volume.
7
Xenograft Tumor Formation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole HCT116 cells and DsRed+ cells were subcutaneously injected into the right and left backs of SCID Beige mice (n = 4 for 500 cells, n = 4 for 1000 cells) in 200 μl DMEM/Matrigel (BD) (1:1). After 4 weeks, the mice were sacrificed.
8
GYKI 52466 Treatment in Hep3B Xenograft Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal protocols were approved by The Johns Hopkins University Animal Care and Use Committee. Male SCID mice 5 to 7 weeks of age were used. Hep3B cells were resuspended at 2.5 × 107 cells/ml in a 1:1 mix of DMEM:Matrigel (BD Biosciences). A 200-μL suspension containing 5×106 cells was implanted subcutaneously into the right flank. Mice were monitored for body weight and tumor volume (mm3), which was calculated as length (mm) × [width (mm)]2 × 0.52. When tumor volume reached 200 mm3 on day 45, mice were randomly divided into control and treatment groups and received daily intraperitoneal injections of vehicle or 5 mg/kg of GYKI 52466, respectively.
9
Xenograft Tumor Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5×105 cells in 10 μl of 1:1 DMEM/Matrigel (BD Biosciences) were injected in the fat pads of female 8-week-old nude mice (Simonsen Laboratories). Once palpable, tumors were measured twice weekly and the tumor volumes were determined as follows: V = 0.52 × (length) 2 × width. All mouse procedures were approved by University of California, San Francisco, Institutional Animal Care and Use Committee.
10
Tumor Induction in Balb/c-nu Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty pathogen-free male Balb/c-nu mice (age, 5–6 weeks) were purchased from the Animal Institute of the Chinese Academy of Medical Science. The animal studies were performed in accordance with standard protocols approved by the Ethics Committee of Hunan Normal University and the Committee of Experimental Animal Feeding and Management (Changsha, China). The mice were randomly divided into five groups (n=4 per group) and maintained under standard conditions, according to typical protocols. The cells were suspended in a serum free-DMEM/Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) mixture (1:1 volume). The mice were inoculated with different quantities of CD133+ SFCs (5×102, 1×103, 5×103, 1×104 and 5×104 cells) in one flank, and unsorted MHCC97 cells (5×104, 1×105, 2×105, 5×105 and 1×106 cells) in the other. Tumorigenicity experiments were terminated two months after cell inoculation. Tumor size was measured using a caliper and the volume was calculated as follows: V (mm3) =L × W2 × 0.5, where L denotes length and W denotes width. The harvested tumors were photographed and weighed immediately. Specimens from tumor tissue samples were fixed in 10% neutral-buffered formalin, processed in paraffin blocks and sectioned. The sections were stained with hematoxylin and eosin (H&E) and examined under an inverted microscope (IX71, Olympus).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!