Ck2 inverted microscope

Manufactured by Olympus

Sourced in Japan, United Kingdom

The CK2 inverted microscope is a laboratory instrument designed for various microscopy applications. It features a reversed optical configuration, allowing for the observation of specimens from below. The CK2 provides clear, high-quality images and supports a range of objective lenses to accommodate different magnification requirements.

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Lab products found in correlation

Transwell inserts, by Avantor (2 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) L glutamine, by Fujifilm (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Hepes, by Fujifilm (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Axioskop 2 plus microscope, by Zeiss (1 mentions) Orca er digital camera, by Hamamatsu Photonics (1 mentions) Crystal violet, by Nanjing Jiancheng (1 mentions) Methocult h4435 enriched, by STEMCELL (1 mentions) Immpact dab peroxidase substrate, by Vector Laboratories (1 mentions) C 5060, by Olympus (1 mentions) Uc6 ultra microtome, by JEOL (1 mentions) 96 well transwell plate, by Corning (1 mentions) Micromanipulator, by Olympus (1 mentions) Coolpix 4500, by Nikon (1 mentions) Facsaria 3 cell sorting system, by BD (1 mentions) Streptavidin hrp conjugate, by GE Healthcare (1 mentions)

Spelling variants of this product

Inverted microscope (2417 citations) CK2 microscope (20 citations) Olympus CK2 Inverted Trinocular Phase Tissue Culture Microscope (2 citations) CK2 phase‐contrast inverted microscope (2 citations)

35 protocols using ck2 inverted microscope

Evaluating Cytotoxicity of AT9283 and AMG900 in CML Cells

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Materials. AT9283 and AMG900 (Adooq Bioscience) were first dissolved in dimethyl sulfoxide (DMSO) to a concentration of 50 mM (stock solution) and stored at -20˚C. These reagents were diluted in phosphate-buffered saline (PBS) before use in the experiments described below.
Cells and culture conditions. The human CML cell line K562 was obtained from the Health Science Research Resources Bank (Osaka, Japan). K562/IR cells were obtained from our laboratory. These cells were maintained in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA) with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.), 2 mM L-glutamine (Wako Pure Chemical Industries, Ltd.), 25 mM HEPES (Wako Pure Chemical Industries, Ltd.), 100 µg/ml penicillin (Gibco; Thermo Fisher Scientific, Inc.), and 100 U/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37˚C in 5% CO 2 .
Trypan blue exclusion assay. CML cells were plated in 96-well plates at a concentration of 2x10 4 cells/ml. Then, AT9283 (10, 30, 50, and 100 nM) or AMG900 (10, 50, 100, 300, and 500 nM) were added to the well. After 3 days, CML cells were stained with trypan blue and the number of stained cells was counted at a magnification of x100 using an Olympus CK2 inverted microscope (Olympus Optical Co.).

Takeda T., Tsubaki M., Genno S., Nemoto C., Onishi Y., Yamamoto Y., Imano M., Satou T, & Nishida S. (2020). AT9283 exhibits antiproliferative effect on tyrosine kinase inhibitor‑sensitive and ‑resistant chronic myeloid leukemia cells by inhibition of Aurora A and Aurora B. Oncology reports, 44(5).

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Angiogenesis Assay with Theaflavin

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Growth Factor-Reduced Matrigel (50 μl; BD Biosciences, San Jose, CA, USA) was added into each well of a 96-well plate and polymerized for 30 min at 37°C. HUVECs (1.5×10⁴/well) in 100 μl conditioned medium (90 μl F12-K medium+10 μl cell culture supernatant of vehicle- or theaflavin derivative-treated cancer cells) were seeded into each Growth Factor-Reduced Matriel-coated well, incubated at 37°C in 5% CO₂ for 6 h, and then photographed using an Olympus CK2 Inverted Microscope (Olympus Optical Co., Tokyo, Japan). Tube length was quantified using NIH ImageJ software (NIH, Bethesda, MD, USA). Tube length was expressed as a percentage compared to that of the control group.

GAO Y., RANKIN G.O., TU Y, & CHEN Y.C. (2016). Inhibitory Effects of the Four Main Theaflavin Derivatives Found in Black Tea on Ovarian Cancer Cells. Anticancer research, 36(2), 643-651.

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Evaluating TrkA Regulation of Eosinophil Adhesion and Migration

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Role of TrkA in regulating eosinophil adhesion to vascular cell adhesion molecule (VCAM)-1 and eotaxin-1-induced migration was evaluated as described previously (23 (link)). In the adhesion assays, the number of adherent cells per field in randomly selected fields (~20 fields/coverslip at ×400 magnification) was manually determined. Next, the number of adherent cells exhibiting spreading was counted and expressed as a percentage of the total number of adherent cells in the field. In the case of migration, migrated cells in a fixed number of randomly selected non-overlapping fields were counted using an Olympus CK2 inverted microscope (200× magnification) and expressed as percent migration relative to migration of vehicle (DMSO)-treated cells.

Dileepan M., Ge X.N., Bastan I., Greenberg Y.G., Liang Y., Sriramarao P, & Rao S.P. (2019). Regulation of eosinophil recruitment and allergic airway inflammation by tropomyosin receptor kinase A. Journal of immunology (Baltimore, Md. : 1950), 204(3), 682-693.

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Microscopic Visualization of H. contortus Larvae

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H. contortus larvae were pipetted onto 2% agarose pads on a microscope slide. Images were captured using an Axioskop 2 Plus microscope (Zeiss), ORCA-ER digital camera (Hamamatsu) and Openlab (Improvision) software at x10 or x40 magnification. Larval development in 96 well plates was assessed using an Olympus CK2 inverted microscope at x20 magnification.

Marks N.D., Winter A.D., Gu H.Y., Maitland K., Gillan V., Ambroz M., Martinelli A., Laing R., MacLellan R., Towne J., Roberts B., Hanks E., Devaney E, & Britton C. (2019). Profiling microRNAs through development of the parasitic nematode Haemonchus identifies nematode-specific miRNAs that suppress larval development. Scientific Reports, 9, 17594.

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Wound Healing Assay in Cell Monolayer

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Cells were seeded at 6 × 10⁵ cells/well in a 6-well plate. Once the cell confluence reached 90%, a wound area was carefully made in the cell monolayer with a sterile 200-µL pipette. The separated cells were washed with PBS. Cells migrated to the injured area and were observed under an Olympus CK-2 inverted microscope and photographed (100 × magnification) at 0, 6, 12, and 24 h.

Wang Y., Tan J., Li J., Chen H, & Wang W. (2021). ING5 Inhibits Migration and Invasion of Esophageal Cancer Cells by Downregulating the IL-6/CXCL12 Signaling Pathway. Technology in Cancer Research & Treatment, 20, 15330338211039940.

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Wound Healing Assay Protocol

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Cells were seeded in six‐well plates at a density of 4 × 10⁵ cells/well. Once the cells reached 90% confluence, a wound area was carefully created by scraping the cell monolayer with a sterile 200 μL pipette tip from one end of the well to the other. The detached cells were removed by washing with phosphate buffered saline. The cells that had migrated to the wounded region were observed using an Olympus CK‐2 inverted microscope (Olympus Corporation, Tokyo, Japan) and photographed (100× magnification) at 0, 8, 16, 20, and 24 hours. The experiments were performed in triplicate.

Liu X., Meng J., Zhang X., Liang X., Zhang F., Zhao G, & Zhang T. (2019). ING5 inhibits lung cancer invasion and epithelial–mesenchymal transition by inhibiting the WNT/β‐catenin pathway. Thoracic Cancer, 10(4), 848-855.

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Wound Healing Assay with A549 Cells

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The A549 EGFR^wt/wt and EGFR^−/− cells were seeded in 6-well plates at a density of 7 × 10⁵ cells per well and starved overnight in DMEM containing 1% FBS. A wound was gently made by scraping the cells with a sterile 200 μL pipette tip. The detached cells were removed using PBS. Pictures were taken at 0 h, 12 h, and 24 h using a CK2 inverted microscope (Olympus, Tokyo, Japan). Experiments were performed in triplicate and repeated at least three times.

Liu B., Song S., Setroikromo R., Chen S., Hu W., Chen D., van der Wekken A.J., Melgert B.N., Timens W., van den Berg A., Saber A, & Haisma H.J. (2019). CX Chemokine Receptor 7 Contributes to Survival of KRAS-Mutant Non-Small Cell Lung Cancer upon Loss of Epidermal Growth Factor Receptor. Cancers, 11(4), 455.

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Wound Healing Assay with Sinomenine

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The assay was performed as described previously [61 (link)]. A549 cells were plated in a 12-well plate and grew to confluence. The monolayer culture was then scrape-wounded with a sterile micropipette tip to create a denuded zone (gap) of a constant width. After removing the cellular debris with PBS, cells were exposed to various concentrations of sinomenine for 24 h. A549 cells that migrated to the wounded region were observed by an Olympus CK-2 inverted microscope and photographed (100× magnification). The wound area was measured by the program Image J (http://rsb.info.nih.gov/ij/). The percentage of wound closure was estimated by the following equation: Wound closure % = [1 − (wound area at T_t/wound area at T₀) × 100%], where T_t is the time after wounding and T₀ is the time immediately after wounding.

Shen K.H., Hung J.H., Liao Y.C., Tsai S.T., Wu M.J, & Chen P.S. (2020). Sinomenine Inhibits Migration and Invasion of Human Lung Cancer Cell through Downregulating Expression of miR-21 and MMPs. International Journal of Molecular Sciences, 21(9), 3080.

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Microspore Developmental Stages and Androgenesis

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The microspore developmental stages were checked using an Olympus CK-2 inverted microscope (Olympus, Southend-on-Sea, UK). The donor tillers containing microspores were collected in their early and mid-uninucleated stages. Cold pre-treatment of tillers was applied to induce in vitro androgenesis. The donor tillers were stored in Erlenmeyer flasks containing tap water, covered with PVC bags and incubated for two weeks at 2–4 °C in a cool room under continuous dim light.

Kruppa J., Kanbar O.Z., Tóth-Lencsés K.A., Kiss E., Bóna L., Lantos C, & Pauk J. (2023). Induction of Triticale (×Triticosecale Wittmack) In Vitro Androgenesis in Anther Cultures of F1 Hybrid Combinations, Varieties and Homogeneity Testing of Offspring Generation. Life, 13(10), 1970.

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Cell Colony Formation Assay

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Cells were seeded in triplicate into 6-well plates at a density of 3×10
² cells/well and cultured for approximately 2 weeks. Cells were fixed with 4% paraformaldehyde and then stained with 1% crystal violet (Jiancheng, Nanjing, China). Cell colony formation was detected with a CK-2 inverted microscope (Olympus, Tokyo, Japan), and the colony number was counted with ImageJ software (NIH, Bethesda, USA).

Yang J., Liu X., Sun Y., Zhang X., Zhao Y., Zhang H., Mei Q., Meng J., Zhang F, & Zhang T. (2023). ING5 overexpression upregulates miR-34c-5p/Snail1 to inhibit EMT and invasion of lung cancer cells: ING5 targets miR-34c-5p/Snail1 axis to inhibit lung cancer invasion. Acta Biochimica et Biophysica Sinica, 55(5), 809-817.

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