Glucose god fs

Manufactured by DiaSys

Sourced in Germany, United States

Glucose GOD FS is a reagent used for the quantitative determination of glucose in human serum, plasma, and other biological samples. It is based on the enzymatic-photometric method and measures the glucose concentration using the glucose oxidase (GOD) principle.

Automatically generated - may contain errors

Lab products found in correlation

Triglycerides fs, by DiaSys (2 mentions) Rat insulin elisa kit, by ALPCO (1 mentions) Cholesterol fs, by DiaSys (1 mentions) Varioskan lux, by Thermo Fisher Scientific (1 mentions) Amyloglucosidase, by Merck Group (1 mentions) Madkmag 71k kit, by Merck Group (1 mentions) Epoch microplate reader, by Agilent Technologies (1 mentions)

9 protocols using glucose god fs

Serum Glucose, Insulin, and HOMA-IR Determination

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was drawn from the antecubital vein, after a fasting period of 12 h. Serum glucose was determined by enzymatic photometric tests (Glucose GOD FS, DiaSys Diagnostic Systems GmbH) in an automated analyzer (Vitalab Selectra E, Vitalab Scientific). Serum insulin levels were determined by an immunoassay method (Adaltis Diagnostics) using an Eclectica analyzer (Adaltis Diagnostics). The cut-off point of 14.38 was considered for abnormal insulin levels since it has been proven to have an adequate performance to diagnose metabolic syndrome for a similar population to our sample [27 (link)].
The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated using the formula [28 (link)]: [fasting insulin (µU/mL)*fasting glucose (mg/dL)]/405. Two validated cut-off points were used to diagnose insulin resistance: HOMA-IR > 3.16 as proposed by Keskin et al. in 2005, which has been widely used in epidemiological studies [29 (link)], and HOMA-IR > 2.97 proposed by Piña-Aguero et al. in 2018 as a valid cut-off for insulin resistance in Mexican adolescents [27 (link)].

Castro-Quezada I., Flores-Guillén E., Núñez-Ortega P.E., Irecta-Nájera C.A., Sánchez-Chino X.M., Mendez-Flores O.G., Olivo-Vidal Z.E., García-Miranda R., Solís-Hernández R, & Ochoa-Díaz-López H. (2019). Dietary Carbohydrates and Insulin Resistance in Adolescents from Marginalized Areas of Chiapas, México. Nutrients, 11(12), 3066.

+ Open protocol

+ Expand

Metabolic Assessment of Offspring

Check if the same lab product or an alternative is used in the 5 most similar protocols

At PND60, blood samples (300 µL) were collected from 6 h fasted offspring. An oral glucose tolerance test (OGTT) was performed in offspring at PND61 and PND62. Following 6 hours of fasting, all rats received a 2 g/kg BW dose of glucose by gavage. Blood samples (100 µL) were collected to determine plasma insulin concentration before (T0) and after 15 (T15) and 30 min (T30) gavage with glucose. Blood glucose was measured at T0, T15, T30, T45, T60, T90, and T120 after glucose intake, using a Performa Accu-Chek® glucometer (Roche Diabetes Care France, Meylan, France).
Insulin (Rat Insulin ELISA kit®, ALPCO, Salem, USA), glucose (Glucose GOD FS®, DiaSys, Holzheim, Germany), triglycerides (Triglycerides FS®, DiaSys, Holzheim, Germany), and cholesterol (Cholesterol FS®, DiaSys, Holzheim, Germany) were measured in plasma, following manufacturers’ instructions. Optical density was read with a microplate reader Varioskan Lux® (ThermoFisher Scientific, Waltham, USA).

Sevrin T., Alexandre-Gouabau M.C., Castellano B., Aguesse A., Ouguerram K., Ngyuen P., Darmaun D, & Boquien C.Y. (2019). Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge. Nutrients, 11(11), 2571.

+ Open protocol

+ Expand

Glycogen Content Quantification in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 24 h with/without 100 nmol/L insulin, total glycogen content was determined using an enzymatic method (amyloglucosidase [Sigma-Aldrich]) and the glucose amount obtained was quantified using a commercial kit (DiaSys “Glucose GOD FS”). All assays were performed in triplicate and were normalized to protein amount (BCA Protein Assay kit, Pierce).

Mizgier M.L., Cataldo L.R., Gutierrez J., Santos J.L., Casas M., Llanos P., Contreras-Ferrat A.E., Moro C., Bouzakri K, & Galgani J.E. (2017). Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion. Journal of Diabetes Research, 2017, 1328573.

+ Open protocol

+ Expand

Multiplex Cytokine Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma IL‐6 and CCL2 levels were measured using a Mouse Adipokine (MADKMAG‐71K) kit, while plasma IFNγ, TNF, IL‐1α, IL‐1β, IL‐2, IL‐7, IL‐10, IL‐15, CCL3, CCL4, CCL5, CXCL1, CXCL9 and CXCL10 from Mouse Cytokine (MCYTMAG‐13K) kit (Millipore, Billerica, MA, USA), according to the manufacturer's instructions. Plasma glucose was measured with Glucose GOD FS (DiaSys, Holzheim, Germany), according to the manufacturer's protocol. The insulin and ALT determination in the plasma, as well as the hepatic triglycerides and hydroxyproline analysis, have been previously described (Rusli et al., 2015, 2016).

Rusli F., Boekschoten M.V., Borelli V., Sun C., Lute C., Menke A.L., van den Heuvel J., Salvioli S., Franceschi C., Müller M, & Steegenga W.T. (2017). Plasticity of lifelong calorie‐restricted C57BL/6J mice in adapting to a medium‐fat diet intervention at old age. Aging Cell, 17(2), e12696.

+ Open protocol

+ Expand

Rapid Plasma Separation and Glucose Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma separation was done within 60 min following blood collection by centrifugation at 3000 rpm for 15 min. Plasma was separated and collected into sterile microtubes, then used for glucose analysis and stored at −20 °C until further analysis. Glucose measurements on blood samples at day 0 (baseline), induction and termination were done in duplicate with the GOD-PAP method according to the manufacturer’s protocol (Glucose GOD FS, DiaSys, Germany). Internal quality control yielded 1.5% precision.

Istikharah R., Nugrahaningsih D.A., Sadewa A.H, & Wahyuningsih M.S. (2022). Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats. The Malaysian Journal of Medical Sciences : MJMS, 29(3), 43-53.

+ Open protocol

+ Expand

Plasma Biomarkers Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was collected into EDTA-coated tubes. Blood samples were placed on ice and centrifuged at 4°C for 10 min at 10,000 g. Plasma was collected and stored at −80°C. To measure plasma MCP-1 concentration a DuoSet ELISA Development kit against mouse MCP-1 was used (R&D Systems, Minneapolis, MN, USA). For insulin measurements an ultra-sensitive mouse insulin ELISA kit was used (Crystal chemicals, Downers Grove, IL, USA). For the other plasma measurements the following kits were used: NEFA Reagent set, Triglycerides Liquicolor, Cholesterol Liquicolor (all Instruchemie, Delfzijl, the Netherlands) and Glucose GOD FS (DiaSys, Holzheim, Germany). All measurements were performed according to the manufacturers’ protocols.

Evers-van Gogh I.J., Oteng A.B., Alex S., Hamers N., Catoire M., Stienstra R., Kalkhoven E, & Kersten S. (2015). Muscle-specific inflammation induced by MCP-1 overexpression does not affect whole-body insulin sensitivity in mice. Diabetologia, 59, 624-633.

+ Open protocol

+ Expand

Corneal Vitality Assessment via Metabolic Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Corneal vitality was assessed by demonstrating metabolic activity. Therefore, the concentrations of glucose (Glucose GOD FS, DiaSys Diagnostic Systems GmbH, Holzheim, Germany) and lactate (Lactate FS, DiaSys Diagnostic Systems GmbH, Holzheim, Germany) were quantified photometrically (EPOCH microplate reader, BioTek Instruments GmbH, Bad Friedrichshall, Germany) in the eluted medium of the anterior chamber after bypassing the corneal endothelium. Low glucose levels indicate consumption of glucose contained in the cell culture medium; conversely, high lactate levels indicate energy consumption. Thus high glucose levels with low lactate levels indicate low energy consumption or cell death as result of toxic action. The glucose/lactate concentrations were analyzed daily.

Panfil C., Chauchat L., Guerin C., Rebika H., Sahyoun M, & Schrage N. (2023). Impact of Latanoprost Antiglaucoma Eyedrops and Their Excipients on Toxicity and Healing Characteristics in the Ex Vivo Eye Irritation Test System. Ophthalmology and Therapy, 12(5), 2641-2655.

+ Open protocol

+ Expand

Quantifying Nitrogen, Glucose, and Minerals

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nitrogen content of EW and EWFs was determined by the Kjeldahl method. Glucose was quantified using an enzymatic spectrophotometric test (Glucose GOD FS) according to the instructions of the provider (DiaSys GmbH, Germany). Mineral quantification by ICP-OES was carried out using samples in 10% iron-free nitric acid (Sigma-Aldrich; 438073), incubated in sealed, plastic tubes at 80 °C overnight with occasional vortexing. Samples were centrifuged (4 °C, 30 min, 18,111g) and supernatants were diluted twofold. The multi-elemental contents of the nitric acid-dissolved sample-solutions were determined using a Perkin Elmer Optima 3000 ICP-OES with radial view and a cross flow nebulizer (Anne Dudley, Analytical Technical Services, University of Reading).

Cochet M.F., Baron F., Bonnassie S., Jan S., Leconte N., Jardin J., Briard-Bion V., Gautier M., Andrews S.C., Guérin-Dubiard C, & Nau F. (2021). Identification of New Antimicrobial Peptides that Contribute to the Bactericidal Activity of Egg White against Salmonella enterica Serovar Enteritidis at 45 °C. Journal of agricultural and food chemistry, 69(7).

+ Open protocol

+ Expand

Colorimetric Enzymatic Serum Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum of the blood was measured using Triglycerides FS (DiaSys Diagnostic Systems, Waterbury, CT, USA) and Glucose GOD FS (DiaSys Diagnostic Systems) based on the colorimetric enzymatic method with glycerol-3-phosphateoxidase and glucose oxidase. Procedure and calculation were performed according to the kit inserts. Briefly, serum was mixed with each provided reagent, homogenized and incubated at room temperature for 10 min. Then the reaction was read with spectrophotometer at absorbance of 500 nm within 60 min.

Tursinawati Y., Hakim R.F., Rohmani A., Kartikadewi A., & Sandra F. (2020). CAPN10 SNP-19 is Associated with Susceptibility of Type 2 Diabetes Mellitus: A Javanese Case-control Study. The Indonesian Biomedical Journal, 12(2), 109-14.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!