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Strep tag 2

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Strep tag II is a short peptide tag used for the identification and purification of recombinant proteins. It can be fused to the N- or C-terminus of a target protein and recognized by Strep-Tactin, a modified streptavidin derivative. This system allows for efficient and gentle affinity-based purification of the tagged protein.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) Propidium iodide, by BD (2 mentions) Streptavidin pe, by BD (2 mentions)

Spelling variants of this product

Strep-tag II mAb (4 citations) Strep-tag (3 citations) Anti-Strep tag II (3 citations) HRP-conjugated Rabbit anti-strep tag II antibody (2 citations)

2 protocols using strep tag 2

1

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Conjugated antibodies specific for CD4, CD8, CD25, CD137, CD45, CD62L,
CD27, CD28 (BD Biosciences), Strep tag II (Genscript), EGFR (ImClone Systems)
and Vβ22 (Beckman Coulter) were used for staining analysis by flow
cytometry. Biotinylated antibodies were stained with Streptavidin-PE (BD
Biosciences, San Jose, CA). T cells transduced with the NY-BR-1 TCR were
analyzed by flow cytometry after staining with antibodies specific for CD8,
Strep-tag II, Vβ22, and MHC-tetramers loaded with NY-BR-1 peptide (FHCRC
Immune Monitoring Lab). Staining with propidium iodide (PI, BD Biosciences) was
used for live/dead cell discrimination as directed by the manufacturer. Flow
analyses were done on a FACS Canto II (Becton Dickinson, Franklin Lakes, NJ).
Data were analyzed using FlowJo software (Treestar).
Liu L., Sommermeyer D., Cabanov A., Kosasih P., Hill T, & Riddell S.R. (2016). Inclusion of Strep-Tag II in design of antigen receptors for T cell immunotherapy. Nature biotechnology, 34(4), 430-434.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Conjugated antibodies specific for CD4, CD8, CD25, CD137, CD45, CD62L,
CD27, CD28 (BD Biosciences), Strep tag II (Genscript), EGFR (ImClone Systems)
and Vβ22 (Beckman Coulter) were used for staining analysis by flow
cytometry. Biotinylated antibodies were stained with Streptavidin-PE (BD
Biosciences, San Jose, CA). T cells transduced with the NY-BR-1 TCR were
analyzed by flow cytometry after staining with antibodies specific for CD8,
Strep-tag II, Vβ22, and MHC-tetramers loaded with NY-BR-1 peptide (FHCRC
Immune Monitoring Lab). Staining with propidium iodide (PI, BD Biosciences) was
used for live/dead cell discrimination as directed by the manufacturer. Flow
analyses were done on a FACS Canto II (Becton Dickinson, Franklin Lakes, NJ).
Data were analyzed using FlowJo software (Treestar).
Liu L., Sommermeyer D., Cabanov A., Kosasih P., Hill T, & Riddell S.R. (2016). Inclusion of Strep-Tag II in design of antigen receptors for T cell immunotherapy. Nature biotechnology, 34(4), 430-434.
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