Passive lysis buffer (plb)
Passive lysis buffer is a solution designed for the gentle extraction and release of cellular contents, including proteins, from biological samples. It facilitates the lysis of cells while preserving the integrity of the target analytes.
Lab products found in correlation
12 protocols using passive lysis buffer (plb)
In Vitro and Eukaryotic Expression of ATP4A and ATP4B
Measuring Notch Signaling Activity
Dual-luciferase Assay for CHCHD2 Promoter
IFNβ Promoter Activation Assay with PR8 NS1 Variants
VEGF-A 3'UTR Regulation by miR-140
Luciferase Assay for miRNA-139-5p Targeting
HIF-2α Transcriptional Activity Assay
Immunoblotting and Immunoprecipitation in LV
Protein Expression Analysis by Western Blotting
Cardiac Protein Expression Analysis
Protein expression was measured using standard immunoblotting methods. Primary antibodies to the cardiac calcium homeostasis proteins sodium calcium exchanger-1 (NCX1, Abcam Inc, Cambridge, MA ab6495); sarcoplasmic calcium ATPase 2a (SERCA2a, Santa Cruz Biotechnology, Santa Cruz, CA, sc-8095); cardiac calsequestrin 2 (CASQ2, Abcam Inc, Cambridge, MA, ab626662); phospholamban (PLB, Thermo Scientific, Nepean, ONT, MA3-922); and phospho-serine 16-specific PLB (pS16-PLB, Millipore, Temecula, CA, 07-052 1) were obtained. Secondary antibodies complexed to horseradish peroxidase and chemiluminescent detection kits were obtained from Pierce Chemical Co., (Rockford, IL). Several exposures from each membrane were collected onto X-ray film. After immunoblotting, membranes were washed, permanently stained with Coomassie Brilliant Blue, destained and scanned. The bands on the X-ray film and stained membrane were scanned and quantitated using Image J software.
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