Acetylthiocholine iodide, 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) were obtained from Merck (Germany), trichloroacetic acid (TCA), tris base, 1,1,3,3-tetramethoxypropane (MDA), 2-thiobarbituric acid (TBA), 2,4,6-tripyridyl-s-triazine (TPTZ), n-butanol, acetic acid, FeCl3-6H2O, benzethonium chloride (Hyamine® 1622), and phosphate buffer were acquired from Sigma-Aldrich (Germany). Analytical grade forms of CHP, DIA and MAL were obtained from local pesticide manufacturing companies. IMOD and ANG were supplied by Rose-Pharmed (Iran). The enzymelinked immunosorbent assay (ELISA) kits were purchased from Bender MedSystem (Germany).
Hyamine 1622
Manufactured by Merck Group
Sourced in United States
Hyamine 1622 is a quaternary ammonium compound commonly used as a preservative and disinfectant in various laboratory applications. It is a colorless liquid with a mild odor. Hyamine 1622 exhibits antimicrobial properties and is effective against a range of microorganisms, including bacteria, fungi, and some viruses.
Automatically generated - may contain errors
Lab products found in correlation
T4 dna ligase, by Thermo Fisher Scientific (2 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Phosphate buffer, by Merck Group (1 mentions)
1 1 3 3 tetramethoxypropane, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
2 thiobarbituric acid, by Merck Group (1 mentions)
N butanol, by Merck Group (1 mentions)
Tris base, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine, by Merck Group (1 mentions)
Fecl3 6h2o, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Miseq sequencing platform, by Illumina (1 mentions)
Butyrylthiocholine iodide, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions)
Riboex, by GeneAll (1 mentions)
3 protocols using hyamine 1622
1
Analytical Methods for Pesticide Detection
2
Genomic DNA Extraction and Sequencing of Nematodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 10,000 IJs were washed 10 times with a 0.8% NaCl solution. Surface sterilization of nematode was done by using 4 mM Hyamine 1622 solution (Sigma-Aldrich, USA) for 30 minutes and again washed with 0.8% NaCl for 3 times (Lu et al. 2017 (link)). The sterilized nematode were flash-frozen and thawed immediately twice for DNA extraction. High molecular weight genomic DNA was extracted using a phenol-chloroform method (Donn et al. 2008 ). The DNA pellet was further resuspended in 100 μl Tris-EDTA buffer. University of Florida’s campus-wide Interdisciplinary Center for Biotechnology Research (ICBR) NextGen DNA Sequencing Core Facility (Gainesville, FL) performed library preparation and sequencing using MiSeq Illumina sequencing platform with 2X300v3 format.
3
Construction and Functional Validation of BChE Plasmid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The research was approved by the ethical committee of Kerman University of Medical Sciences and the Ethics Approval Code is IR.KMU.REC.1398.294. Restriction enzymes, T4 DNA ligase, GeneJET Plasmid Miniprep® kit (#K0502, #K0503), and Taq DNA Polymerase Master Mix RED were purchased from Thermo Scientific (Germany) and Ampliqon® (Denmark) respectively. Plasmids pCMV3-BChE (Cat No., HG12010-UT) and pBudCE4.1 (Cat No., V532-20) were provided by Sino Biological (China) and Invitrogen (USA). RiboEx™ (Cat No., 301–001) was purchased from GeneAll (Portugal). Parstous cDNA synthesis kit (Cat No., A101161) was provided by Parstous Biotechnology company (Mashhad, Iran). SinaSYBR Blue HS-qPCRMix, 2x (Cat No., MM2171) was provided by Sinaclone (Tehran, Iran). 5.5′-Dithiobis(2-nitrobenzoic acid) DTNB (Cat No., D218200), Hyamine 1622 (Cat No., 121–54-0), and butyrylthiocholine iodide (Cat No., B3253) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Gibco (Grand Island, USA). Fetal bovine serum (FBS) and penicillin streptomycin were purchased from Hangzhou Sijiqing Biological Engineering Materials Co. Ltd (Hangzhou, China). The HEK-293 cell line was provided from Pasteur Institute (Tehran, Iran).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!