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Mntbap

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MnTBAP is a manganese-based compound used in various laboratory applications. It functions as a superoxide dismutase (SOD) mimetic, catalyzing the dismutation of superoxide radicals. The product is designed for research purposes only and its specific applications may vary depending on the research objectives.

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Lab products found in correlation

Cycloheximide (chx), by Merck Group (3 mentions) N acetyl cysteine (nac), by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Euk 134, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Nis elements ar 3, by Nikon (1 mentions) Eclipse ti s inverted microscope, by Nikon (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Ds fi1 camera, by Nikon (1 mentions) Petri dishes, by Avantor (1 mentions) Mannitol, by Fujifilm (1 mentions) Na2s2, by Dojindo Laboratories (1 mentions) Na2s3, by Dojindo Laboratories (1 mentions) Na2s4, by Dojindo Laboratories (1 mentions) Anti lc3b antibody, by Merck Group (1 mentions) Temozolomide, by Merck Group (1 mentions) Tomm20, by Abcam (1 mentions) Cox 4, by Abcam (1 mentions) Bnip3, by Abcam (1 mentions)

Spelling variants of this product

Manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) (5 citations)

30 protocols using mntbap

1

Macrophage Differentiation of U937 Cells

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To induce macrophage differentiation of U937 monocytes, the cells were suspended at 500,000 cells/ml in petri dishes (VWR International, Radnor, PA) and stimulated for 48 hours with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, St. Louis, MO). In separate experiments, U937 cells were stimulated concurrently with 5 μg/ml recombinant human high mobility group box1 protein (HMGB1, R&D systems, Minneapolis, MN) and recombinant human tumor necrosis factor alpha (TNFα), interleukin 1 beta (IL-1β), and IL-6 (10 ng/ml each, R& D Systems). Differentiation assays were performed in the presence or absence of 1.0 μg/ml of human rSTC-1 (BioVendor Research and Diagnostic Products, Asheville, NC or Czech Republic), 1 mM of the superoxide donor paraquat (Sigma-Aldrich), and/or 100 μM of the cell-permeable superoxide dismutase mimetic MnTBAP (Sigma-Aldrich). After 48 hours, images of the cells were captured on a Nikon Eclipse Ti-S inverted microscope using a Ds-Fi1 camera (Nikon, Melville, NY) and managed with NiS Elements AR 3.0 software (Nikon). The differentiated macrophages were subsequently harvested, counted, and processed for real-time reverse transcription polymerase chain reaction (RT-PCR) or flow cytometry analysis.
Mohammadipoor A., Lee R.H., Prockop D.J, & Bartosh T.J. (2016). Stanniocalcin-1 Attenuates Ischemic Cardiac Injury and Response of Differentiating Monocytes/Macrophages to Inflammatory Stimuli. Translational research : the journal of laboratory and clinical medicine, 177, 127-142.
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2

Cardiomyocyte Glucose and Oxidative Stress

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Cardiomyocytes were incubated for 24 h to establish adhesion to either PAA gel substrates or glass coverslips, and were subsequently treated with various concentrations of glucose (5, 10, 15 or 25 mM) in the presence or absence of ROS scavengers, such as N-acetyl cysteine (NAC; 0.25 and 1 mM, Sigma) and manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP; 0.1 mM, Sigma) for 24 h. To investigate the effect of removing accumulated ROS, cardiomyocytes were first exposed to a high level of glucose for 24 h, followed by treatment with 5 mM glucose in the presence or absence of NAC. Where indicated, cells were treated with mannitol (WAKO), instead of glucose, to evaluate the effect of high osmolality, which could also be induced by high-glucose treatment.
Morishima M., Horikawa K, & Funaki M. (2018). Cardiomyocytes cultured on mechanically compliant substrates, but not on conventional culture devices, exhibit prominent mitochondrial dysfunction due to reactive oxygen species and insulin resistance under high glucose. PLoS ONE, 13(8), e0201891.
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3

Cutaneous Vascular Tissue Biopsy and Analysis

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In a subset of participants (n=8 HC, n=8 MDD), two 3 mm cutaneous vascular tissue samples were obtained from the ventral forearm under local anesthesia (2% lidocaine without epinephrine) via punch biopsy, immediately snap frozen in liquid nitrogen, and stored at −80°C until analysis34 (link), 40 (link). Protein abundance/expression was determined by Western blot and total reactive oxygen species and reactive nitrogen species (ROS+RNS) production was quantified fluorometrically (Cell Biolabs Inc, San Diego, CA, USA). The relative contribution of superoxide to total ROS+RNS was assessed by pretreatment with the SOD mimetic MnTBAP (Sigma-Aldrich). Details are provided in the Online Data Supplement.
Greaney J.L., Saunders E.F., Santhanam L, & Alexander L.M. (2019). Oxidative Stress Contributes to Microvascular Endothelial Dysfunction in Men and Women with Major Depressive Disorder. Circulation research, 124(4), 564-574.
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4

Sulfide Signaling Pathway Protocol

Cited 1 time
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SSP4 (3′,6′-di(O-thiosalicyl)fluorescein), Na2S2, Na2S3, and Na2S4 were purchased from Dojindo molecular Technologies Inc. (Rockville, MD, USA). MnTBAP, which is also a Mn porphyrin, but lacks appreciable SOD-mimetic activity [26 (link)] was purchased from Sigma–Aldrich (St. Louis, MO, USA), other MnPs were synthesized as previously reported [27 (link),28 (link)]. All other chemicals were purchased either from Sigma-Aldrich or ThermoFisher Scientific (Grand Island, NY, USA). Please note that we use H2S to denote the total sulfide added (sum of H2S + HS−) usually derived from Na2S. Also, while S2− is often thought as part of the H2S + HS− equilibrium, it does not exist under these conditions as the pK > 12 [29 (link)]. Phosphate buffer (PBS; in mM): 137 NaCl, 2.7 KCl, 8 Na2HPO4, 2 NaH2PO4. pH was adjusted with 10 mM HCl or NaOH to pH 7.4.
Olson K.R., Gao Y., Arif F., Patel S., Yuan X., Mannam V., Howard S., Batinic-Haberle I., Fukuto J., Minnion M., Feelisch M, & Straub K.D. (2019). Manganese Porphyrin-Based SOD Mimetics Produce Polysulfides from Hydrogen Sulfide. Antioxidants, 8(12), 639.
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5

Temozolomide Combination Therapy Protocol

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Temozolomide (TMZ), glutathione (GSH), metalloporphyrin Mn(III) tetrakis(4-benzoic acid)porphyrin (MnTBAP), and anti-LC3B antibodies were purchased from Sigma (St. Louis, MO). TMZ was dissolved in DMSO to a storage concentration of 50 mmol/L. Primary antibodies against the following proteins were purchased from Abcam (Cambridge, MA): FOXO3a, BNIP3, ATG5, p62 (SQSTM1), AIF, TOMM20, COXIV, VDAC1, phospho-H2AX at Ser139, HIF-α and H2A. Anti-β-Actin antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Other reagents were purchased from Sigma (St. Louis, MO).
He C., Lu S., Wang X.Z., Wang C.C., Wang L., Liang S.P., Luo T.F., Wang Z.C., Piao M.H., Chi G.F, & Ge P.F. (2021). FOXO3a protects glioma cells against temozolomide-induced DNA double strand breaks via promotion of BNIP3-mediated mitophagy. Acta Pharmacologica Sinica, 42(8), 1324-1337.
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6

Induction of Ferroptosis and Apoptosis

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Erastin was purchased from Selleck (Shanghai, China). The mPTP blockers including sanglifehrin A, cyclosporin A and bongkrekic acid were purchased from Sigma (Shanghai, China). The anti-oxidant MnTBAP was also from Sigma. The caspase-3 specific inhibitor z-DEVD-fmk and the caspase-9 specific inhibitor z-LEHD-fmk were purchased from Calbiochem (Darmstadt, Germany). All antibodies applied in this study were obtained from Santa Cruz Biotech (Shanghai, China).
Huo H., Zhou Z., Qin J., Liu W., Wang B, & Gu Y. (2016). Erastin Disrupts Mitochondrial Permeability Transition Pore (mPTP) and Induces Apoptotic Death of Colorectal Cancer Cells. PLoS ONE, 11(5), e0154605.
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7

Synthesis and Sources of Reagents

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The synthesis of racemic fluorizoline was performed as previously described [2 ]. Actinomycin D was purchased from Enzo Life Sciences (Farmingdale, New York, USA). Q-VD-OPh and Z-IETD-FMK were from R&D systems (Minneapolis, Minnesota, USA). Recombinant mouse TNFα was from PeproTech (Rocky Hill, New Jersey, USA). MnTBAP, etoposide and cycloheximide were from Sigma-Aldrich (Saint Louis, Missouri, USA).
Moncunill-Massaguer C., Saura-Esteller J., Pérez-Perarnau A., Palmeri C.M., Núñez-Vázquez S., Cosialls A.M., González-Gironès D.M., Pomares H., Korwitz A., Preciado S., Albericio F., Lavilla R., Pons G., Langer T., Iglesias-Serret D, & Gil J. (2015). A novel prohibitin-binding compound induces the mitochondrial apoptotic pathway through NOXA and BIM upregulation. Oncotarget, 6(39), 41750-41765.
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8

Synthesized Compounds for Cellular Research

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Fluorizoline and compound 2a were synthesized as described in [1 (link)]. Thapsigargin, ISRIB, antimycin A, rocaglamide A, MnTBAP, and EGTA/AM (EGTA) were from Sigma-Aldrich (Saint Louis, MO, USA) ATP was from Roche (Basel, Switzerland). BAPTA/AM was from Abcam (Cambridge, UK). IN-8 and SB203580 were from Calbiochem (Merck-Millipore, Darmstadt, Germany).
Saura-Esteller J., Sánchez-Vera I., Núñez-Vázquez S., Cosialls A.M., Gama-Pérez P., Bhosale G., Mendive-Tapia L., Lavilla R., Pons G., Garcia-Roves P.M., Duchen M.R., Iglesias-Serret D, & Gil J. (2021). Activation of the Integrated Stress Response and ER Stress Protect from Fluorizoline-Induced Apoptosis in HEK293T and U2OS Cell Lines. International Journal of Molecular Sciences, 22(11), 6117.
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9

Mitochondrial Fission and Oxidative Stress

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Antibodies to FUNDC1 (Aviva System Biology, diluted 1:500), Drp1 (clone D6C7, Cell Signaling cat. #8570 diluted 1:1000), Ser616-phosphorylated Drp1 (Cell Signaling cat. #3455 diluted 1:1000), Drp1 (Cell Signaling cat. #5391 diluted 1:1000), Tyr925-phosphorylated FAK (Cell Signaling cat. #3284 diluted 1:1000), FAK (Cell Signaling cat. n. #3285), FLAG (clone M-2, Sigma cat. #F1804 diluted 1:5000) and β-actin (clone AC-15, Sigma cat. #A5441 diluted 1:100000) were used for Western blotting. Antibodies to F-Actin (Invitrogen cat. #A12379, Phalloidin, diluted 1:300), Tom20 (FL-145 Santa Cruz, cat. # sc-11415 diluted 1:250), and anti-mitochondria antibody (clone 113–1 Millipore cat. # MAB1273 diluted 1:1000) were used for immunofluorescence. For immunofluorescence experiments, Phalloidin Alexa488, MitoTracker Red CMH2XROS, MitoSOX Red (Molecular Probes), mitochondrial superoxide dismutase mimetic MnTBAP and cycloheximide (CHX) was from Sigma.
Li J., Agarwal E., Bertolini I., Seo J.H., Caino M.C., Ghosh J.C., Kossenkov A.V., Liu Q., Tang H.Y., Goldman A.R., Languino L.R., Speicher D.W, & Altieri D.C. (2020). The mitophagy effector FUNDC1 controls mitochondrial reprogramming and cellular plasticity in cancer cells. Science signaling, 13(642), eaaz8240.
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10

Quantifying Cellular Oxidative Stress

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MSM-mEos2 cells were induced with ATc (500 ng/ml) and incubated for 12 h under standard growth conditions. N-Acetyl cysteine (NAC) (10 μM), an oxidative agent that reduces the level of in situ reactive oxygen species (ROS), was added to the induced MSM-mEos2 cultures and incubated at 37°C for 90 min to prepare the negative control. Along with the negative control, another set of induced cultures were treated with a ROS-inducing compound, 100 μM MnTBAP (catalog no. 55266-18-7; Sigma-Aldrich) and incubated for 15 min to prepare the positive control. CellRox orange (catalog no. C10443; Thermo Fisher), was added to the induced samples and control and incubated for 30 min at room temperature. FACS analysis was performed to measure the CellRox orange intensity. ROS-positive and -negative gates were determined with control samples, and ROS levels in dim and lit cell population were determined with reference to the controls.
Srinivas V., Arrieta-Ortiz M.L., Kaur A., Peterson E.J, & Baliga N.S. (2020). PerSort Facilitates Characterization and Elimination of Persister Subpopulation in Mycobacteria. mSystems, 5(6), e01127-20.
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