Carboxyl-terminated
beads (50 nm diameter), N-hydroxysuccinimide (NHS),
1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide (EDC), and the plastic
pH indicator strip were purchased from Sigma-Aldrich (Dorset, UK).
Self-adhesive magnet sheets were purchased from Polarity Magnets Company
(Wickford, Essex, UK). The P. aeruginosa peptide substrate NH2-Ahx-Gly-Gly-Gly-Ahx-Cys was synthesized by
Pepmic Co. Ltd (Suzhou, China). BHI broth and agar were purchased
from SDA, Oxoid Ltd (Basingstoke, UK). Sterile filters (0.22 μm)
were obtained from Millipore (Watford, UK). The wash/storage buffer
(10 mM Tris base, 150 mM sodium chloride, 0.1% (w/v) bovine serum
albumin, 1 mM ethylenediaminetetraacetic acid, 0.1% sodium azide,
pH 7.5) and the coupling buffer (10 mM potassium phosphate, 0.15 M
sodium chloride, pH 5.5) were prepared from chemicals of analytical
grade.
beads (50 nm diameter), N-hydroxysuccinimide (NHS),
1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide (EDC), and the plastic
pH indicator strip were purchased from Sigma-Aldrich (Dorset, UK).
Self-adhesive magnet sheets were purchased from Polarity Magnets Company
(Wickford, Essex, UK). The P. aeruginosa peptide substrate NH2-Ahx-Gly-Gly-Gly-Ahx-Cys was synthesized by
Pepmic Co. Ltd (Suzhou, China). BHI broth and agar were purchased
from SDA, Oxoid Ltd (Basingstoke, UK). Sterile filters (0.22 μm)
were obtained from Millipore (Watford, UK). The wash/storage buffer
(10 mM Tris base, 150 mM sodium chloride, 0.1% (w/v) bovine serum
albumin, 1 mM ethylenediaminetetraacetic acid, 0.1% sodium azide,
pH 7.5) and the coupling buffer (10 mM potassium phosphate, 0.15 M
sodium chloride, pH 5.5) were prepared from chemicals of analytical
grade.