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Stat3 sirna

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STAT3 siRNA is a small interfering RNA (siRNA) designed to target the STAT3 gene. STAT3 siRNA is used to temporarily knock down the expression of the STAT3 gene in cell-based studies.

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Lab products found in correlation

Control sirna, by Santa Cruz Biotechnology (4 mentions) Lipofectamine transfection reagent, by Thermo Fisher Scientific (2 mentions) Stattic, by Santa Cruz Biotechnology (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) Opti mem medium, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) Transfection reagent, by iNtRON Biotechnology (1 mentions) P stat3 antibody, by Santa Cruz Biotechnology (1 mentions) Rnaimax opti mem, by Thermo Fisher Scientific (1 mentions) X tremegene hp transfection reagent, by Roche (1 mentions) Nf κb p65 sirna, by Santa Cruz Biotechnology (1 mentions) Cav 3 sirna, by Santa Cruz Biotechnology (1 mentions) Akt sirna, by Santa Cruz Biotechnology (1 mentions) P akt, by Santa Cruz Biotechnology (1 mentions) Rt pcr primers, by Eurofins (1 mentions) P stat3, by Santa Cruz Biotechnology (1 mentions) Revertaid cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)

21 protocols using stat3 sirna

1

Knockdown of STAT3 and NF-κB in A-549 Cells

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For the study, VCAM-1 siRNA, STAT3 siRNA, NF-κB (p65) siRNA or STAT3 siRNA along with respective scramble control were procured from Santa Cruz biotech, USA. The transfecting the cells with siRNA, the A-549 cells were trypsinized in suspension and 3×105 cells were transferred in 6 well plates. The cells received transfection of STAT3 siRNA and NF-κB siRNA following the manufacturers protocol, the cells were collected after defined time intervals for further study. Western blot analysis was done for confirming the transfection.
Li L., Li D, & Chen Y. (2020). miRNA-26a blocks interleukin-2-mediated migration and proliferation of non-small cell lung cancer cells via vascular cell adhesion molecule-1. Translational Cancer Research, 9(3), 1768-1778.
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2

STAT3 Silencing in Primary HUVEC

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Primary HUVEC were seeded in six-well plates the day before transfection. RNAiMAX (Invitrogen, Carlsbad, CA) was used to transfect 50 nM of STAT3 siRNA (Santa Cruz Biotechnology, Santa Cruz, CA, USA) into the cells in OPTI-MEM Medium (Invitrogen) for 48 h. Control siRNA containing a scrambled sequence (Santa Cruz) was used as a negative control.
Sui Y.B., Wang Y., Liu L., Liu F, & Zhang Y.Q. (2019). Astragaloside IV alleviates heart failure by promoting angiogenesis through the JAK-STAT3 pathway. Pharmaceutical Biology, 57(1), 48-54.
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3

Knockdown of STAT3 in A549 and H460 cells

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The A549 and H460 cells were seeded in 6-well plates at 1 × 106 cells per well, grown to 60% confluence, and transfected with STAT3 siRNA (sc-29493; Santa Cruz Biotechnology, Dallas, TX, USA) using the lipofectamine transfection reagent (Thermo Fisher Scientific, Inc., Waltham, MA, USA). After 24 h, the transfected cells were then cultured with or without UA for another 24 h under the same cell culture conditions.
Kang D.Y., Sp N., Lee J.M, & Jang K.J. (2021). Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer Cells. Biomedicines, 9(3), 297.
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4

STAT3 Knockdown in HCCLM3 Cells

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siRNA transfection was carried out as described earlier [55] (link). To determine whether BT interferes with EMT through modulating STAT3 signaling, HCCLM3 cells were transfected with STAT3 siRNA (Santa Cruz Biotechnology [sc-29493]) and scrambled control with transfection reagent (Intron Biotechnology, Seoul, Korea).
Lee J.H., Mohan C.D., Deivasigamani A., Jung Y.Y., Rangappa S., Basappa S., Chinnathambi A., Alahmadi T.A., Alharbi S.A., Garg M., Lin Z.X., Rangappa K.S., Sethi G., Hui K.M, & Ahn K.S. (2020). Brusatol suppresses STAT3-driven metastasis by downregulating epithelial-mesenchymal transition in hepatocellular carcinoma. Journal of Advanced Research, 26, 83-94.
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5

Stat3 Knockdown Using siRNA

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siRNA or control were transfected using Lipofectamine2000 (Thermo Fisher Scientific) following manufacturer’s guidance. Stat3 siRNA, primers for Stat3 mRNA, Stattic, and p-STAT3 antibody were purchased from Santa Cruz Biotechnology.
Zhu G., Mei L., Vishwasrao H.D., Jacobson O., Wang Z., Liu Y., Yung B.C., Fu X., Jin A., Niu G., Wang Q., Zhang F., Shroff H, & Chen X. (2017). Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy. Nature Communications, 8, 1482.
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6

Transfection and Stimulation of Macrophages

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Macrophages were transfected using a slightly modified protocol [19] (link). Briefly, 80 µM STAT3 siRNA (Santa Cruz) or Silencer Select Negative Control (Invitrogen) in RNAiMax+OptiMEM (Invitrogen) was added to 1×106 macrophages in antibiotic-free RPMI1640 medium. Cells were incubated overnight at 37°C, rinsed the next day to remove dead cells and treated with IL-4+IL-13±IL-6 for 48 h. Cells were collected for analysis via western blot or arginase assay, or stimulated with LPS to assess nitric oxide production.
Fernando M.R., Reyes J.L., Iannuzzi J., Leung G, & McKay D.M. (2014). The Pro-Inflammatory Cytokine, Interleukin-6, Enhances the Polarization of Alternatively Activated Macrophages. PLoS ONE, 9(4), e94188.
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7

Transient Knockdown of PIAS1 and STAT3 in DCs

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Selected CD11c+ DC (1.5×106) were
transiently transfected in 6-well plates using X-tremeGENE HP transfection
reagent following the manufacturer’s instructions (Roche, Indianapolis,
IN). PIAS1 or STAT-3 siRNA was combined with transfection reagent at 4:1 ratio
(μl reagent:μg of plasmid). The transfection solution was added to
the DC culture and cells were incubated at 37° C. For transient
knockdown, transfections of siRNA were performed twice (40 and 24 hours) before
additional treatment with IFN-γ or LPS. PIAS1 siRNA, STAT3 siRNA, and
control siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz
Biotechnology, Santa Cruz, CA).
Hanke N.T., LaCasse C.J., Larmonier C.B., Alizadeh D., Trad M., Janikashvili N., Bonnotte B., Katsanis E, & Larmonier N. (2014). PIAS1 and STAT-3 impair the tumoricidal potential of IFN-γ-stimulated mouse dendritic cells generated with IL-15. European journal of immunology, 44(8), 2489-2499.
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8

Cardioprotective Effects of Pharmacological Interventions

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Embryonic rat cardiomyocyte-derived H9C2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS in a humidified atmosphere (5% CO2) at 37°C. Commercial Cav-3 siRNA, Akt siRNA, and STAT3 siRNA (Santa Cruz Biotechnology) were used for inhibiting Cav-3, Akt, and STAT3 protein expression, respectively, according to the manufacturer's instructions. After transfection with control, Cav-3 siRNA, Akt siRNA, or STAT3 siRNA, cells were treated with N-acetylcysteine (NAC, 1 mmol/L [29 (link)]) or combination with remifentanil (2.5 μM [12 (link)]) in HG condition for 36 h; then, the cells were exposed to H/R stimulation.
Lei S., Su W., Xia Z.Y., Wang Y., Zhou L., Qiao S., Zhao B., Xia Z, & Irwin M.G. (2019). Hyperglycemia-Induced Oxidative Stress Abrogates Remifentanil Preconditioning-Mediated Cardioprotection in Diabetic Rats by Impairing Caveolin-3-Modulated PI3K/Akt and JAK2/STAT3 Signaling. Oxidative Medicine and Cellular Longevity, 2019, 9836302.
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9

Stat3 Knockdown in Mouse Primary CFs

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Mouse primary CFs were seeded on plates and cultured to 80% confluence, then transfected with Stat3 siRNA (#sc-29494) or control siRNA (#sc-37007, both Santa Cruz Biotechnology, CA) by using Lipofectamine RNAi MAX transfection reagent (Thermo Fisher Scientific, MA) for 24 hr.
Bao Q., Zhang B., Suo Y., Liu C., Yang Q., Zhang K., Yuan M., Yuan M., Zhang Y, & Li G. (2020). Intermittent hypoxia mediated by TSP1 dependent on STAT3 induces cardiac fibroblast activation and cardiac fibrosis. eLife, 9, e49923.
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10

Angiogenic and Transcriptional Regulation

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DMEM-high glucose medium and Fetal bovine serum (FBS) were obtained from Invitrogen (NY, USA). Purified anti-mouse antibodies (VEGF, HIF1α, Sp1, Sp3, p300, CBP, pAKT, pERK, STAT3, pSTAT3) and STAT3 siRNA were procured from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-mouse/rabbit fluorescence conjugated secondary antibodies (FITC and PE conjugate) were purchased from Sigma Aldrich (St. Louis, US). RT-PCR primers were designed and procured from Eurofins, Bangalore, India. Trizol reagent for RNA isolation and Revert Aid™ cDNA synthesis kit were procured from Invitrogen (Carlsbad, CA, USA) and Fermentas (Waltham, MA, USA), respectively.
Saha A., Nandi P., Dasgupta S., Bhuniya A., Ganguly N., Ghosh T., Guha I., Banerjee S., Baral R, & Bose A. (2020). Neem Leaf Glycoprotein Restrains VEGF Production by Direct Modulation of HIF1α-Linked Upstream and Downstream Cascades. Frontiers in Oncology, 10, 260.
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